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Mouse Genetic Background and Human Hematopoietic Stem Cells Biology; Tips for Humanization

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Humanized Mice for HIV Research

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Abstract

In past, many efforts have been made to develop immunodeficient mice that are permissive for human cell or tissue engraftment, and to model human hemato-lymphoid system (HHLS) for understanding human physiology and diseases. However, mouse environment remains suboptimal to robust maintenance of human hematopoietic stem cells (HSCs), and full development and function of human hematopoiesis and immunity. Thus, this limitation impedes long-term study of human immune function, especially in context of human immunodeficient virus (HIV) infection. Here, we summarize the recent findings on genetic and molecular determinants for efficient HHLS development in mice, and discuss how to improve humanization in order to sustain human hematopoiesis and enhance human immune function.

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Authors and Affiliations

  1. Division of Hematology, University Hospital Zurich, CH-8091, Zurich, Switzerland

    Larisa V. Kovtonyuk & Hitoshi Takizawa

  2. International Research Center for Medical Sciences, Kumamoto University, 860-0811, Kumamoto, Japan

    Hitoshi Takizawa

Authors
  1. Larisa V. Kovtonyuk
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  2. Hitoshi Takizawa
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Correspondence to Hitoshi Takizawa .

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Editors and Affiliations

  1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska, USA

    Larisa Y. Poluektova

  2. Center for AIDS Research, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

    J. Victor Garcia

  3. Research Center for AIDS Laboratory of Viral Pathogenesis, Kyoto University Institute for Virus Research, Sakyo-ku, Japan

    Yoshio Koyanagi

  4. Division of Hematology, University Hospital Zürich, Zürich, Switzerland

    Markus G. Manz

  5. Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA

    Andrew M. Tager

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Kovtonyuk, L., Takizawa, H. (2014). Mouse Genetic Background and Human Hematopoietic Stem Cells Biology; Tips for Humanization. In: Poluektova, L., Garcia, J., Koyanagi, Y., Manz, M., Tager, A. (eds) Humanized Mice for HIV Research. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1655-9_4

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