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Non-apoptotic Sperm Selection

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Non-Invasive Sperm Selection for In Vitro Fertilization

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Abstract

Apoptosis plays an important role in the pathogenesis of male infertility. Failure of fertilization in assisted reproduction (ART) despite the use of morphologically normal and motile spermatozoa may be due to an ongoing process of apoptosis. Therefore, the use of apoptotic spermatozoa may be one of the reasons behind the suboptimal success rates in ART. Sperm selection methods currently used routinely in conjunction with ART procedures lack the ability to specifically target apoptotic spermatozoa. To address this deficiency, a protocol that includes the selection of non-apoptotic spermatozoa using magnetic activated cell sorting (MACS) has been proposed.

MACS in combination with density gradient centrifugation proved to yield spermatozoa with higher quality in terms of motility, morphology, viability, apoptosis markers, DNA integrity, oocyte penetration potential, and sperm chromatin decondensation. Several reports documented the beneficial effects of applying MACS as a sperm selection method on ART outcomes. Specifically, pregnancy rates were significantly higher following intrauterine inseminations and intracytoplasmic sperm injection using spermatozoa selected by MACS. Healthy live births were also reported following the application of MACS as a sperm selection method. However, before the technology could be routinely adopted in clinical practice, its safety should be carefully examined.

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Authors and Affiliations

  1. Andrology Laboratory & Reproductive Tissue Bank, The Toronto Institute for Reproductive Medicine – ReproMed, 56 Aberfoyle Crescent, Toronto, ON, Canada, M8X-2W4

    Tamer Said MD, PhD, HCLD/CC

  2. Translational Haematology/Oncology, Taussig Cancer Institute, Cleveland Clinic, Euclid Avenue, Desk R-40, Rm:R04-35, Cleveland, OH, 44195, USA

    Reda Z. Mahfouz MD, PhD

  3. Embryology Laboratory, The Toronto Institute for Reproductive Medicine – ReproMed, 56 Aberfoyle Cres, Toronto, ON, Canada, M8X 2W4

    Iryna Kuznyetsova PhD, HCLD

  4. The Toronto Institute for Reproductive Medicine – ReproMed, 56 Aberfoyle Cres, Toronto, ON, Canada, M8X 2W4

    Alfonso P. Del Valle MD, FRCS(C)

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  1. Tamer Said MD, PhD, HCLD/CC
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  2. Reda Z. Mahfouz MD, PhD
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  3. Iryna Kuznyetsova PhD, HCLD
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  4. Alfonso P. Del Valle MD, FRCS(C)
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Correspondence to Tamer Said MD, PhD, HCLD/CC .

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Editors and Affiliations

  1. Cleveland Clinic, Andrology Center & Center for Reproductive Medicine, Cleveland, Ohio, USA

    Ashok Agarwal

  2. Fertility - Centro de Fertilização Assistida, São Paulo, São Paulo, Brazil

    Edson Borges Jr.

  3. Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, São Paulo, São Paulo, Brazil

    Amanda S. Setti

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Said, T., Mahfouz, R.Z., Kuznyetsova, I., Del Valle, A.P. (2015). Non-apoptotic Sperm Selection. In: Agarwal, A., Borges Jr., E., Setti, A. (eds) Non-Invasive Sperm Selection for In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1411-1_7

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