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Sperm Selection Based on Surface Electrical Charge

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Non-Invasive Sperm Selection for In Vitro Fertilization

Abstract

Sperm selection is an important part of the ICSI and is based solely on sperm morphology and motility. The latter parameters may not be sufficient to select sperm with intact chromatin. Therefore, sperm selected based on sperm functional characteristics may result in selection of the most appropriate sperm for the ICSI procedure. In this chapter, we introduce two advanced sperm selection procedures based on electric charge on the sperm plasma membrane, Zeta method and electrophoretic method, and also discuss the importance of these methods for ART.

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Abbreviations

DGC:

Density gradient centrifugation

ROS:

Reactive oxygen species

GPI:

Glycosylphosphatidylinositol

TUNEL:

Terminal deoxynucleotidyl transferase dUTP nick end labeling

AOT:

Acridine orange test

PS:

Phosphatidylserine

EPS:

External phosphatidylserine

HA:

Hyaluronic acid

MACS:

Magnetic-activated cell sorting

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Author information

Authors and Affiliations

  1. Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

    Mohammad Hossein Nasr-Esfahani PhD & Tavalaee Marziyeh

  2. Isfahan Fertility and Infertility Center, Isfahan, Iran

    Mohammad Hossein Nasr-Esfahani PhD

Authors
  1. Mohammad Hossein Nasr-Esfahani PhD
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  2. Tavalaee Marziyeh
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Corresponding author

Correspondence to Mohammad Hossein Nasr-Esfahani PhD .

Editor information

Editors and Affiliations

  1. Cleveland Clinic, Andrology Center & Center for Reproductive Medicine, Cleveland, Ohio, USA

    Ashok Agarwal

  2. Fertility - Centro de Fertilização Assistida, São Paulo, São Paulo, Brazil

    Edson Borges Jr.

  3. Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, São Paulo, São Paulo, Brazil

    Amanda S. Setti

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Nasr-Esfahani, M.H., Marziyeh, T. (2015). Sperm Selection Based on Surface Electrical Charge. In: Agarwal, A., Borges Jr., E., Setti, A. (eds) Non-Invasive Sperm Selection for In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1411-1_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1411-1_4

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1410-4

  • Online ISBN: 978-1-4939-1411-1

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