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Intracytoplasmic Morphologically Selected Sperm Injection (IMSI): Indications and Clinical Results

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Non-Invasive Sperm Selection for In Vitro Fertilization

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Abstract

High magnification opens different approaches of sperm head morphology examination.

To understand the correlation between sperm normalcy, fertilization, and early embryo development we established, a detailed sperm scoring classification according to strict morphology criteria; permitting to discard the worst spermatozoon, in real time, prior intracytoplasmic sperm injection.

Our consideration of maternal age correlated to sperm score revealed a difference between oocytes from women younger than 30 and oocytes from elderly women.

We showed that high-magnified spermatozoa, in cases of Robertsonian translocation carriers cannot be used to select sperm cells with a balanced chromosomal content.

However, there is a significant correlation between sperm-head morphology assessed by high magnification and sperm chromatin-decondensation rate of specific kind of spermatozoa.

This suggests that this spermatozoon should not be selected for intracytoplasmic sperm injection and must be discarded.

The high magnified spermatozoon observation seems to impact the birth outcome leading to lower risks of major malformation mainly affecting urogenital system and more often boys.

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Acknowledgement

J.R. Kovac is an NIH K12 scholar supported by a Male Reproductive Health Research (MRHR) Career Development Physician-Scientist Award (HD073917-01) from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) Program awarded to Dolores J. Lamb (DJL). DJL is supported by NIH grants P01HD36289 from the Eunice Kennedy Shriver NICHD and 1R01DK078121 from the National Institute of Kidney and Digestive Diseases.

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Authors and Affiliations

  1. Art Unit, Drouot Laboratory, Drouot Street 21, Paris, 75009, France

    Nino Guy Cassuto MD & André Hazout MD

Authors
  1. Nino Guy Cassuto MD
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  2. André Hazout MD
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Correspondence to Nino Guy Cassuto MD .

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Editors and Affiliations

  1. Cleveland Clinic, Andrology Center & Center for Reproductive Medicine, Cleveland, Ohio, USA

    Ashok Agarwal

  2. Fertility - Centro de Fertilização Assistida, São Paulo, São Paulo, Brazil

    Edson Borges Jr.

  3. Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, São Paulo, São Paulo, Brazil

    Amanda S. Setti

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Cassuto, N.G., Hazout, A. (2015). Intracytoplasmic Morphologically Selected Sperm Injection (IMSI): Indications and Clinical Results. In: Agarwal, A., Borges Jr., E., Setti, A. (eds) Non-Invasive Sperm Selection for In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1411-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1411-1_16

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