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Non-Invasive Sperm Selection for In Vitro Fertilization pp 149–155Cite as

MSOME and Sperm Chromosomal Constitution

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Abstract

In the past decade a new approach involving real-time, high-magnification (up to 6,600×) observation of unstained spermatozoa, named "motile sperm organelle morphology examination" (MSOME) has been introduced. MSOME is able to identify mainly sperm head vacuoles, considered as nuclear defects that may be associated with DNA and chromosomal abnormalities. Because all the available tests for functional and genetic sperm assessment are extremely cytotoxic, sperm DNA integrity and chromosomal constitution cannot be assessed in the sperm cell used for intracytoplasmic morphologically selected sperm injection (ICSI); therefore, several studies have investigated the relationship between sperm morphology by MSOME and sperm chromosomal status. This chapter reviews the current literature on the MSOME and sperm chromosomal constitution.

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References

  1. Smit M, Romijn JC, Wildhagen MF, Weber RF, Dohle GR. Sperm chromatin structure is associated with the quality of spermatogenesis in infertile patients. Fertil Steril. 2010;94(5):1748–52.

    CAS  PubMed  Google Scholar 

  2. Trost LW, Nehra A. Guideline-based management of male infertility: why do we need it? Ind J Urol. 2011;27(1):49–57.

    Google Scholar 

  3. Merchant R, Gandhi G, Allahbadia GN. In vitro fertilization/intracytoplasmic sperm injection for male infertility. Ind J Urol. 2011;27(1):121–32.

    Google Scholar 

  4. Bonde JP, Ernst E, Jensen TK, Hjollund NH, Kolstad H, Henriksen TB, et al. Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners. Lancet. 1998;352(9135):1172–7.

    CAS  PubMed  Google Scholar 

  5. Van Waart J, Kruger TF, Lombard CJ, Ombelet W. Predictive value of normal sperm morphology in intrauterine insemination (IUI): a structured literature review. Hum Reprod Update. 2001;7(5):495–500.

    PubMed  Google Scholar 

  6. Kruger TF, Menkveld R, Stander FS, Lombard CJ, Van der Merwe JP, van Zyl JA, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 1986;46(6):1118–23.

    CAS  PubMed  Google Scholar 

  7. Lundin K, Soderlund B, Hamberger L. The relationship between sperm morphology and rates of fertilization, pregnancy and spontaneous abortion in an in-vitro fertilization/intracytoplasmic sperm injection programme. Hum Reprod. 1997;12(12):2676–81.

    CAS  PubMed  Google Scholar 

  8. Barroso G, Valdespin C, Vega E, Kershenovich R, Avila R, Avendano C, et al. Developmental sperm contributions: fertilization and beyond. Fertil Steril. 2009;92(3):835–48.

    CAS  PubMed  Google Scholar 

  9. Host E, Ernst E, Lindenberg S, Smidt-Jensen S. Morphology of spermatozoa used in IVF and ICSI from oligozoospermic men. Reprod Biomed Online. 2001;3(3):212–5.

    PubMed  Google Scholar 

  10. Lopes S, Sun JG, Jurisicova A, Meriano J, Casper RF. Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril. 1998;69(3):528–32.

    CAS  PubMed  Google Scholar 

  11. Munne S, Magli C, Bahce M, Fung J, Legator M, Morrison L, et al. Preimplantation diagnosis of the aneuploidies most commonly found in spontaneous abortions and live births: XY, 13, 14, 15, 16, 18, 21, 22. Prenat Diagn. 1998;18(13):1459–66.

    CAS  PubMed  Google Scholar 

  12. Sakkas D, Urner F, Bianchi PG, Bizzaro D, Wagner I, Jaquenoud N, et al. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod. 1996;11(4):837–43.

    CAS  PubMed  Google Scholar 

  13. Bartoov B, Berkovitz A, Eltes F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001;345(14):1067–8.

    CAS  PubMed  Google Scholar 

  14. Verma RS, Babu A. Human chromosomes: manual of basic techniques. New York, NY: Pergamon Press; 1989.

    Google Scholar 

  15. Barlow P, Vosa CG. The Y chromosome in human spermatozoa. Nature. 1970;226(5249):961–2.

    CAS  PubMed  Google Scholar 

  16. Pawlowitzki IH, Pearson PL. Chromosomal aneuploidy in human spermatozoa. Humangenetik. 1972;16(1):119–22.

    CAS  PubMed  Google Scholar 

  17. Rudak E, Jacobs PA, Yanagimachi R. Direct analysis of the chromosome constitution of human spermatozoa. Nature. 1978;274(5674):911–3.

    CAS  PubMed  Google Scholar 

  18. Ko E, Rademaker A, Martin R. Microwave decondensation and codenaturation: a new methodology to maximize FISH data from donors with very low concentrations of sperm. Cytogenet Cell Genet. 2001;95(3–4):143–5.

    CAS  PubMed  Google Scholar 

  19. Templado C, Vidal F, Estop A. Aneuploidy in human spermatozoa. Cytogenet Genome Res. 2011;133(2–4):91–9.

    CAS  PubMed  Google Scholar 

  20. Bourrouillou G, Dastugue N, Colombies P. Chromosome studies in 952 infertile males with a sperm count below 10 million/ml. Hum Genet. 1985;71(4):366–7.

    CAS  PubMed  Google Scholar 

  21. Van Assche E, Bonduelle M, Tournaye H, Joris H, Verheyen G, Devroey P, et al. Cytogenetics of infertile men. Hum Reprod. 1996;11 Suppl 4:1–24. discussion 25–26.

    PubMed  Google Scholar 

  22. Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21(1):33–44.

    CAS  PubMed  Google Scholar 

  23. Zini A, Libman J. Sperm DNA damage: clinical significance in the era of assisted reproduction. CMAJ. 2006;175(5):495–500.

    PubMed Central  PubMed  Google Scholar 

  24. Shi Q, Martin RH. Aneuploidy in human spermatozoa: FISH analysis in men with constitutional chromosomal abnormalities, and in infertile men. Reproduction. 2001;121(5):655–66.

    CAS  PubMed  Google Scholar 

  25. Shi Q, Martin RH. Aneuploidy in human sperm: a review of the frequency and distribution of aneuploidy, effects of donor age and lifestyle factors. Cytogenet Cell Genet. 2000;90(3–4):219–26.

    CAS  PubMed  Google Scholar 

  26. Loutradi KE, Tarlatzis BC, Goulis DG, Zepiridis L, Pagou T, Chatziioannou E, et al. The effects of sperm quality on embryo development after intracytoplasmic sperm injection. J Assist Reprod Genet. 2006;23(2):69–74.

    PubMed Central  PubMed  Google Scholar 

  27. Egozcue S, Blanco J, Vendrell JM, Garcia F, Veiga A, Aran B, et al. Human male infertility: chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion. Hum Reprod Update. 2000;6(1):93–105.

    CAS  PubMed  Google Scholar 

  28. Garolla A, Fortini D, Menegazzo M, De Toni L, Nicoletti V, Moretti A, et al. High-power microscopy for selecting spermatozoa for ICSI by physiological status. Reprod Biomed Online. 2008;17(5):610–6.

    PubMed  Google Scholar 

  29. de Almeida Ferreira Braga DP, Setti AS, Figueira RC, Nichi M, Martinhago CD, Iaconelli Jr A, et al. Sperm organelle morphologic abnormalities: contributing factors and effects on intracytoplasmic sperm injection cycles outcomes. Urology. 2011;78(4):786–91.

    PubMed  Google Scholar 

  30. Perdrix A, Travers A, Chelli MH, Escalier D, Do Rego JL, Milazzo JP, et al. Assessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles. Hum Reprod. 2011;26(1):47–58.

    CAS  PubMed  Google Scholar 

  31. Watanabe S, Tanaka A, Fujii S, Mizunuma H, Fukui A, Fukuhara R, et al. An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay. Hum Reprod. 2011;26(5):978–86.

    CAS  PubMed  Google Scholar 

  32. Boitrelle F, Ferfouri F, Petit JM, Segretain D, Tourain C, Bergere M, et al. Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod. 2011;26(7):1650–8.

    CAS  PubMed  Google Scholar 

  33. Guthauser B, Vialard F, Dakouane M, Izard V, Albert M, Selva J. Chromosomal analysis of spermatozoa with normal-sized heads in two infertile patients with macrocephalic sperm head syndrome. Fertil Steril. 2006;85(3):750.e755–7.

    Google Scholar 

  34. Chelli MH, Albert M, Ray PF, Guthauser B, Izard V, Hammoud I, et al. Can intracytoplasmic morphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm head syndrome? Fertil Steril. 2010;93(4):1347.e1341–1345.

    Google Scholar 

  35. Cassuto NG, Le Foll N, Chantot-Bastaraud S, Balet R, Bouret D, Rouen A, et al. Sperm fluorescence in situ hybridization study in nine men carrying a Robertsonian or a reciprocal translocation: relationship between segregation modes and high-magnification sperm morphology examination. Fertil Steril. 2011;96(4):826–32.

    PubMed  Google Scholar 

  36. Haaf T, Ward DC. Higher order nuclear structure in mammalian sperm revealed by in situ hybridization and extended chromatin fibers. Exp Cell Res. 1995;219(2):604–11.

    CAS  PubMed  Google Scholar 

  37. Zalensky AO, Allen MJ, Kobayashi A, Zalenskaya IA, Balhorn R, Bradbury EM. Well-defined genome architecture in the human sperm nucleus. Chromosoma. 1995;103(9):577–90.

    CAS  PubMed  Google Scholar 

  38. Zalensky A, Zalenskaya I. Organization of chromosomes in spermatozoa: an additional layer of epigenetic information? Biochem Soc Trans. 2007;35(Pt 3):609–11.

    CAS  PubMed  Google Scholar 

  39. Perdrix A, Travers A, Clatot F, Sibert L, Mitchell V, Jumeau F, et al. Modification of chromosomal architecture in human spermatozoa with large vacuoles. Andrology. 2013;1(1):57–66.

    CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, Av. Brigadeiro Luis Antonio, 4545, Sao Paulo, SP, 01401-002, Brazil

    Amanda S. Setti BSc

  2. Fertility - Centro de Fertilização Assistida, Av. Brigadeiro Luis Antonio, 4545, Sao Paulo, SP, 01401-002, Brazil

    Edson Borges Jr. MD, PhD

Authors
  1. Amanda S. Setti BSc
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  2. Edson Borges Jr. MD, PhD
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Corresponding author

Correspondence to Edson Borges Jr. MD, PhD .

Editor information

Editors and Affiliations

  1. Cleveland Clinic, Andrology Center & Center for Reproductive Medicine, Cleveland, Ohio, USA

    Ashok Agarwal

  2. Fertility - Centro de Fertilização Assistida, São Paulo, São Paulo, Brazil

    Edson Borges Jr.

  3. Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, São Paulo, São Paulo, Brazil

    Amanda S. Setti

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Setti, A.S., Borges, E. (2015). MSOME and Sperm Chromosomal Constitution. In: Agarwal, A., Borges Jr., E., Setti, A. (eds) Non-Invasive Sperm Selection for In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1411-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-1411-1_15

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1410-4

  • Online ISBN: 978-1-4939-1411-1

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