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Collection, Processing, and Banking of Umbilical Cord Blood

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Perinatal Stem Cells

Abstract

The blood in the umbilical cord and placenta after the birth of a child is comparable to bone marrow for use in hematopoietic stem cell transplantation and offered a number of advantages. In the past 20 years, more than 30,000 cord blood transplants have been performed worldwide. Stem cell transplantation for hematological malignancies and genetic disorders however, is an uncommon occurrence. Research performed by several independent laboratories has demonstrated that cord blood also contains a mixture of pluripotent stem cells capable of giving rise to cells derived from the endodermal, mesodermal, and ectodermal lineages. Thus, CB is a readily available stem cell source for use in tissue engineering and regenerative medicine applications, which are hypothesized to be more frequent events than the need for hematopoietic stem cell transplant. This chapter will review the methodologies for collection, processing, and banking these cells for these future clinical uses.

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Acknowledgements

I would like to acknowledge the invaluable technical assistance obtained from all of the Cord Blood Bank personnel that have made this review possible. I would also like to acknowledge the numerous physicians, midwifes, and nurses that have participated in the collection of the cord blood and tissue units. In addition, the author gratefully acknowledges the assistance of Katherine S. Brown and Heather Brown at Cord Blood Registry in the preparation of this manuscript.

Conflict of Interest 

The author is a consultant to CBR Systems, Inc. and Chief Science Officer for Adicyte, Inc.

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Authors and Affiliations

  1. Department of Immunobiology, University of Arizona, 1501 North Campbell Avenue, Room 6122 AHSC, 245221, Tucson, AZ, 85724, USA

    David T. Harris Ph.D.

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  1. David T. Harris Ph.D.
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Correspondence to David T. Harris Ph.D. .

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Editors and Affiliations

  1. Wake Forest Baptist Medical Center, Institute for Regenerative Medicine, Winston-Salem, North Carolina, USA

    Anthony Atala

  2. Wake Forest Baptist Medical Center, Institute for Regenerative Medicine, Winston-Salem, North Carolina, USA

    Sean V. Murphy

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Harris, D.T. (2014). Collection, Processing, and Banking of Umbilical Cord Blood. In: Atala, A., Murphy, S. (eds) Perinatal Stem Cells. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1118-9_24

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