Abstract
Toxoplasmosis is a disease caused by the protozoan parasite Toxoplasma gondii. This parasite is transmitted by ingestion of cysts found in the tissues of infected animals or by ingestion of food or water contaminated with oocysts released in the feces of infected cats. T. gondii is able to infect all warm-blooded animals, including humans. Vaccination against T. gondii is the most efficient and safe method to prevent this infectious disease. Since the portal of entry of T. gondii is the mucosa, an efficient stimulation of the mucosa and an adequate systemic response constitute a priority which could be accomplished by the administration of an oral or nasal vaccine. In this context, plant-based expression systems represent an interesting production platform for oral vaccine development due to their reduced manufacturing costs and high scalability. However, the heterologous expression of parasitic antigens in plants has been poorly explored. Despite the lack of interest aroused by the proteins derived from parasites to be expressed in plants, the results shown in the present chapter are auspicious. The antigens of T. gondii in plants orally administered in low doses and without the use of adjuvants have been shown to be able to induce a protective immune response. In fact, the degree of protection observed is similar to that reported by other authors who assayed these proteins as recombinant proteins or DNA vaccines in combination with adjuvants. Both GRA4 and SAG1 expressed in plants are good candidates for the development of a multi-antigenic vaccine against Toxoplasma. In summary, the expression of T. gondii antigens in plants is a realistic strategy to develop an anti-T. gondii vaccine. The joint expression of SAG1 and GRA4 in plants may provide an excellent opportunity to explore the application of an oral vaccine against toxoplasmosis based on edible plant tissues.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Angus CW, Klivington-Evans D, Dubey JP, Kovacs JA (2000) Immunization with a DNA plasmid encoding the SAG1 (P30) protein of Toxoplasma gondii is immunogenic and protective in rodents. J Infect Dis 181(1):317â324
Bonenfant C, Dimier-Poisson I, Velge-Roussel F, Buzoni-Gatel D, Del Giudice G, Rappuoli R et al (2001) Intranasal immunization with SAG1 and nontoxic mutant heat-labile enterotoxins protects mice against Toxoplasma gondii. Infect Immun 69(3):1605â1612
Bourguin I, ChardĂšs T, Bout D (1993) Oral immunization with Toxoplasma gondii antigens in association with cholera toxin induces enhanced protective and cell-mediated immunity in C57BL/6 mice. Infect Immun 61(5):2082â2088
Bout DT, Mevelec MN, Velge-Roussel F, Dimier-Poisson I, Lebrun M (2002) Prospects for a human Toxoplasma vaccine. Curr Drug Targets Immune Endocr Metabol Disord 2(3):227â234
Brynska A, Tomaszewicz-Libudzic E, Wolanczyk T (2001) Obsessive-compulsive disorder and acquired toxoplasmosis in two children. Eur Child Adolesc Psychiatry 10(3):200â204
Burg JL, Perelman D, Kasper LH, Ware PL, Boothroyd JC (1988) Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol 141(10):3584â3591
Buxton D, Innes EA (1995) A commercial vaccine for ovine toxoplasmosis. Parasitology 110(Suppl):S11â16
Buxton D, Thomson K, Maley S, Wright S, Bos HJ (1991) Vaccination of sheep with a live incomplete strain (S48) of Toxoplasma gondii and their immunity to challenge when pregnant. Vet Rec 129(5):89â93
Caetano BC, Bruña-Romero O, Fux B, Mendes EA, Penido ML, Gazzinelli RT (2006) Vaccination with replication-deficient recombinant adenoviruses encoding the main surface antigens of Toxoplasma gondii induces immune response and protection against infection in mice. Hum Gene Ther 17(4):415â426
Carruthers VB, Giddings OK, Sibley LD (1999) Secretion of micronemal proteins is associated with toxoplasma invasion of host cells. Cell Microbiol 1(3):225â35
Capron A, Dessaint JP (1988) Vaccination against parasitic diseases: some alternative concepts for the definition of protective antigens. Ann Inst Pasteur Immunol 139(1):109â117
Cesbron-Delauw MF (1994) Dense-granule organelles of Toxoplasma gondii: their role in the host-parasite relationship. Parasitol Today 10(8):293â296
Cesbron-Delauw MF, Capron A (1993) Excreted/secreted antigens of Toxoplasma gondii-their origin and role in the host-parasite interaction. Res Immunol 144(1):41â44
Cenci-Goga BT, Rossitto PV, Sechi P, McCrindle CM, Cullor JS (2011) Toxoplasma in animals, food, and humans: an old parasite of new concern. Foodborne Pathog Dis 8(7):751â762
Chen XG, Gong Y, Hua-Li, Lun ZR, Fung MC (2001) High-level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli. Protein Expr Purif 23(1):33â37
Chen G, Chen H, Guo H, Zheng H (2002) Protective effect of DNA-mediated immunization with a combination of SAG1 and IL-2 gene adjuvant against infection of Toxoplasma gondii in mice. Chin Med J (Engl) 115(10):1448â1452
Chen R, Lu SH, Tong QB, Lou D, Shi DY, Jia BB et al (2009) Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii. J Zhejiang Univ Sci B 10(7):512â521
Clemente M, Corigliano MG (2012) Overview of plant-made vaccine antigens against malaria. J Biomed Biotechnol 2012:206918
Clemente M, Curilovic R, Sassone A, Zelada A, Angel SO, Mentaberry AN (2005) Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity. Mol Biotechnol 30(1):41â50
Cong H, Gu QM, Jiang Y, He SY, Zhou HY, Yang TT et al (2005) Oral immunization with a live recombinant attenuated Salmonella typhimurium protects mice against Toxoplasma gondii. Parasite Immunol 27(1â2):29â35
Cong H, Zhang M, Xin Q, Wang Z, Li Y, Zhao Q et al (2013) Compound DNA vaccine encoding SAG1/SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii. Parasit Vectors 6:63
Cook AJ, Gilbert RE, Buffolano W, Zufferey J, Petersen E, Jenum PA et al (2000) Sources of toxoplasma infection in pregnant women: European multicentre case-control study. European research network on congenital toxoplasmosis. BMJ 321(7254):142â147
Couper KN, Nielsen HV, Petersen E, Roberts F, Roberts CW, Alexander J (2003) DNA vaccination with the immunodominant tachyzoite surface antigen (SAG-1) protects against adult acquired Toxoplasma gondii infection but does not prevent maternofoetal transmission. Vaccine 21(21â22):2813â2820
Couvreur G, Sadak A, Fortier B, Dubremetz JF (1988) Surface antigens of Toxoplasma gondii. Parasitology 97(Pt 1):1â10
Del L YĂĄcono M, Farran I, Becher ML, Sander V, SĂĄnchez VR, MartĂn V et al (2012) A chloroplast-derived Toxoplasma gondii GRA4 antigen used as an oral vaccine protects against toxoplasmosis in mice. Plant Biotechnol J 10(9):1136â1144
Desai PN, Shrivastava N, Padh H (2010) Production of heterologous proteins in plants: strategies for optimal expression. Biotechnol Adv 28(4):427â435
Desolme B, MĂ©vĂ©lec MN, Buzoni-Gatel D, Bout D (2000) Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene. Vaccine 18(23):2512â2521
Dubey JP (2000) Sources of Toxoplasma gondii infection in pregnancy. Until rates of congenital toxoplasmosis fall, control measures are essential. BMJ 321(7254):127â128
Dubey JP (2008) The history of Toxoplasma gondii-the first 100 years. J Eukaryot Microbiol 55(6):467â475
Dziadek B, Gatkowska J, Brzostek A, Dziadek J, Dzitko K, Grzybowski M et al (2011) Evaluation of three recombinant multi-antigenic vaccines composed of surface and secretory antigens of Toxoplasma gondii in murine models of experimental toxoplasmosis. Vaccine 29(4):821â830
Dziadek B, Gatkowska J, Grzybowski M, Dziadek J, Dzitko K, Dlugonska H (2012) Toxoplasma gondii: the vaccine potential of three trivalent antigen-cocktails composed of recombinant ROP2, ROP4, GRA4 and SAG1 proteins against chronic toxoplasmosis in BALB/c mice. Exp Parasitol 131(1):133â138
Fachado A, RodrĂguez A, Angel SO, Pinto DC, Vila I, Acosta A, Amendoeira RR, Lanses-Vieira J (2003) Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice. Vaccine 21:1327â1335
Fang R, Feng H, Hu M, Khan MK, Wang L, Zhou Y et al (2012) Evaluation of immune responses induced by SAG1 and MIC3 vaccine cocktails against Toxoplasma gondii. Vet Parasitol 187(1â2):140â146
Ferguson DJ, Jacobs D, Saman E, Dubremetz JF, Wright SE (1999) In vivo expression and distribution of dense granule protein 7 (GRA7) in the exoenteric (tachyzoite, bradyzoite) and enteric (coccidian) forms of Toxoplasma gondii. Parasitology 119(Pt 3):259â265
Ferraro G, Becher ML, Angel SO, Zelada A, Mentaberry AN, Clemente M (2008) Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves. Exp Parasitol 120(1):118â122
Fischer HG, Stachelhaus S, Sahm M, Meyer HE, Reichmann G (1998) GRA7, an excretory 29 kDa Toxoplasma gondii dense granule antigen released by infected host cells. Mol Biochem Parasitol 91(2):251â262
Garcia JL, Navarro IT, Biazzono L, Freire RL, da Silva GuimarĂŁes Junior J, Cryssafidis AL et al (2007) Protective activity against oocyst shedding in cats vaccinated with crude rhoptry proteins of the Toxoplasma gondii by the intranasal route. Vet Parasitol 145(3â4):197â206
Guerina NG, Hsu HW, Meissner HC, Maguire JH, Lynfield R, Stechenberg B et al (1994) Neonatal serologic screening and early treatment for congenital Toxoplasma gondii infection. The new england regional Toxoplasma working group. N Engl J Med 330(26):1858â1863
Haumont M, Delhaye L, Garcia L, Jurado M, Mazzu P, Daminet V et al (2000) Protective immunity against congenital toxoplasmosis with recombinant SAG1 protein in a guinea pig model. Infect Immun 68(9):4948â4953
Hefferon KL (2010) The mucosal immune response to plant-derived vaccines. Pharm Res 27(10):2040â2042
Hefferon KL (2012) Recent patents in plant biotechnology: impact on global health. Recent Pat Biotechnol 6(2):97â105
Henriquez FL, Woods S, Cong H, McLeod R, Roberts CW (2010) Immunogenetics of Toxoplasma gondii informs vaccine design. Trends Parasitol 26(11):550â555
Hill D, Dubey JP (2002) Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect 8(10):634â640
HiszczyĆska-Sawicka E, Li H, Xu JB, Oledzka G, Kur J, Bickerstaffe R, Stankiewicz M (2010) Comparison of immune response in sheep immunized with DNA vaccine encoding Toxoplasma gondii GRA7 antigen in different adjuvant formulations. Exp Parasitol 124(4):365â372
HiszczyĆska-Sawicka E, OlÄdzka G, Holec-GÄ sior L, Li H, Xu JB, Sedcole R et al (2011) Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii. Vet Parasitol 177(3â4):281â289
Holland GN (2003) Ocular toxoplasmosis: a global reassessment. Part I: epidemiology and course of disease. Am J Ophthalmol 136(6):973â988
Hoseinian Khosroshahi K, Ghaffarifar F, DâSouza S, Sharifi Z, Dalimi A (2011) Evaluation of the immune response induced by DNA vaccine cocktail expressing complete SAG1 and ROP2 genes against toxoplasmosis. Vaccine 29(4):778â783
Huynh MH, Rabenau KE, Harper JM, Beatty WL, Sibley LD, Carruthers VB (2003) Rapid invasion of host cells by Toxoplasma requires secretion of the MIC2-M2AP adhesive protein complex. EMBO J 22(9):2082â2090
Igarashi M, Kano F, Tamekuni K, Kawasaki PM, Navarro IT, Vidotto O, Vidotto MC, Machado RZ, Garcia JL (2008) Toxoplasma gondii: cloning, sequencing, expression, and antigenic characterization of ROP2, GRA5 and GRA7. Genet Mol Res 7(2):305â313
Innes EA, Vermeulen AN (2006) Vaccination as a control strategy against the coccidial parasites Eimeria, Toxoplasma and Neospora. Parasitology 133(Suppl):S145â168
Innes EA, Bartley PM, Maley S, Katzer F, Buxton D (2009) Veterinary vaccines against Toxoplasma gondii. Mem Inst Oswaldo Cruz 104(2):246â251
Innes EA (2010) A brief history and overview of Toxoplasma gondii. Zoonoses Public Health 57(1):1â7
Jacob SS, Cherian S, Sumithra TG, Raina OK, Sankar M (2013) Edible vaccines against veterinary parasitic diseases-current status and future prospects. Vaccine 31(15):1879â1885
Jacobs D, Dubremetz JF, Loyens A, Bosman F, Saman E (1998) Identification and heterologous expression of a new dense granule protein (GRA7) from Toxoplasma gondii. Mol Biochem Parasitol 91(2):237â249
Jacobs D, Vercammen M, Saman E (1999) Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain. Clin Diagn Lab Immunol 6(1):24â9
Jones JL, Dubey JP (2012) Foodborne toxoplasmosis. Clin Infect Dis 55(6):845â851
Jongert E, de Craeye S, Dewit J, Huygen K (2007) GRA7 provides protective immunity in cocktail DNA vaccines against Toxoplasma gondii. Parasite Immunol 29(9):445â453
Jongert E, Melkebeek V, De Craeye S, Dewit J, Verhelst D, Cox E (2008) An enhanced GRA1-GRA7 cocktail DNA vaccine primes anti-toxoplasma immune responses in pigs. Vaccine 26(8):1025â1031
Karg SR, Kallio PT (2009) The production of biopharmaceuticals in plant systems. Biotechnol Adv 27(6):879â894
Kasper LH, Crabb JH, Pfefferkorn ER (1983) Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody. J Immunol 130(5):2407â2412
Kasper LH, Bradley MS, Pfefferkorn ER (1984) Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol 132(1):443â449
Khan IA, Eckel ME, Pfefferkorn ER, Kasper LH (1988) Production of gamma interferon by cultured human lymphocytes stimulated with a purified membrane protein (P30) from Toxoplasma gondii. J Infect Dis 157(5):979â984
Kijlstra A, Jongert E (2008) Control of the risk of human toxoplasmosis transmitted by meat. Int J Parasitol 38(12):1359â1370
Kortbeek LM, Hofhuis A, Nijhuis CD, Havelaar AH (2009) Congenital toxoplasmosis and DALYsin the Netherlands. Mem Inst Oswaldo Cruz 104(2):370â373
Kur J, Holec-Gasior L, HiszczyĆska-Sawicka E (2009) Current status of toxoplasmosis vaccinedevelopment. Expert Rev Vaccines 8(6):791â808
Labruyere E, Lingnau M, Mercier C, Sibley LD (1999) Differential membrane targeting of the secretory proteins GRA4 and GRA6 within the parasitophorous vacuole formed by Toxoplasma gondii. Mol Biochem Parasitol 102(2):311â324
LaguĂa-Becher M, MartĂn V, Kraemer M, Corigliano M, Yacono ML, Goldman A et al (2010) Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice. BMC Biotechnol 10:52
Lakshmi PS, Verma D, Yang X, Lloyd B, Daniell H (2013) Low cost tuberculosis vaccine antigens in capsules: expression in chloroplasts, bio-encapsulation, stability and functional evaluation in vitro. PLoS ONE 8(1):e54708
Lebrun M, Michelin A, El Hajj H, Poncet J, Bradley PJ, Vial H et al (2005) The rhoptry neck protein RON4 re-localizes at the moving junction during Toxoplasma gondii invasion. Cell Microbiol 7(12):1823â1833
Lekutis C, Ferguson DJ, Grigg ME, Camps M, Boothroyd JC (2001) Surface antigens of Toxoplasma gondii: variations on a theme. Int J Parasitol 31(12):1285â1292
Levine ND (1977) Tazonomy of Toxoplasma. J Protozool 24(1):36â41
Li WS, Chen QX, Ye JX, Xie ZX, Chen J, Zhang LF (2011) Comparative evaluation of immunization with recombinant protein and plasmid DNA vaccines of fusion antigen ROP2 and SAG1 from Toxoplasma gondii in mice: cellular and humoral immune responses. Parasitol Res 109(3):637â644
Liljeqvist S, StĂ„hl S (1999) Production of recombinant subunit vaccines: protein immunogens, live delivery systems and nucleic acid vaccines. J Biotechnol 73(1):1â33
Liu KY, Zhang DB, Wei QK, Li J, Li GP, Yu JZ (2006) Biological role of surface Toxoplasma gondii antigen in development of vaccine. World J Gastroenterol 12(15):2363â2368
Liu Q, Gao S, Jiang L, Shang L, Men J, Wang Z et al (2008) A recombinant pseudorabies virus expressing TgSAG1 protects against challenge with the virulent Toxoplasma gondii RH strain and pseudorabies in BALB/c mice. Microbes Infect 10(12â13):1355â1362
Liu Q, Shang L, Jin H, Wei F, Zhu XQ, Gao H (2010) The protective effect of a Toxoplasma gondii SAG1 plasmid DNA vaccine in mice is enhanced with IL-18. Res Vet Sci 89(1):93â97
Lössl AG, Waheed MT (2011) Chloroplast-derived vaccines against human diseases: achievements, challenges and scopes. Plant Biotechnol J 9(5):527â539
Luft BJ, Remington JS (1992) Toxoplasmic encephalitis in AIDS. Clin Infect Dis 15(2):211â222
Martin V, Supanitsky A, Echeverria PC, Litwin S, Tanos T, De Roodt AR et al (2004) Recombinant GRA4 or ROP2 protein combined with alum or the gra4 gene provides partial protection in chronic murine models of toxoplasmosis. Clin Diagn Lab Immunol 11(4):704â710
Mendes ĂA, Fonseca FG, CasĂ©rio BM, Colina JP, Gazzinelli RT, Caetano BC (2013) Recombinant vaccines against T. gondii: comparison between homologous and heterologous vaccination protocols using two viral vectors expressing SAG1.PLoS One 8(5):e63201
Meng M, He S, Zhao G, Bai Y, Zhou H, Cong H, Lu G, Zhao Q, Zhu XQ. (2012) Evaluation of protective immune responses induced by DNA vaccines encoding Toxoplasma gondii: surface antigen 1 (SAG1) and 14-3-3 protein in BALB/c mice. Parasit Vectors 5:273
Menzies FM, Henriquez FL, Roberts CW (2008) Immunological control of congenital toxoplasmosis in the murine model. Immunol Lett 15(2):83â89
Mevelec MN, ChardĂšs T, Mercereau-Puijalon O, Bourguin I, Achbarou A, Dubremetz JF et al (1992) Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies. Mol Biochem Parasitol 56(2):227â238
MĂ©vĂ©lec MN, Mercereau-Puijalon O, Buzoni-Gatel D, Bourguin I, ChardĂšs T, Dubremetz JF et al (1998) Mapping of B epitopes in GRA4, a dense granule antigen of Toxoplasma gondii and protection studies using recombinant proteins administered by the oral route. Parasite Immunol 20(4):183â195
MĂ©vĂ©lec MN, Bout D, Desolme B, Marchand H, MagnĂ© R, Bruneel O et al (2005) Evaluation of protective effect of DNA vaccination with genes encoding antigens GRA4 and SAG1 associated with GM-CSF plasmid, against acute, chronical and congenital toxoplasmosis in mice. Vaccine 23(36):4489â4499
Mercier C, Dubremetz JF, Rauscher B, Lecordier L, Sibley LD, Cesbron-Delauw MF (2002) Biogenesis of nanotubular network in Toxoplasma parasitophorous vacuole induced by parasite proteins. Mol Biol Cell 13(7):2397â2409
Miman O, Kusbeci OY, Aktepe OC, Cetinkaya Z (2010) The probable relation between Toxoplasma gondii and Parkinsonâs disease. Neurosci Lett 475(3):129â131
Min J, Qu D, Li C, Song X, Zhao Q, Li XA et al (2012) Enhancement of protective immune responses induced by Toxoplasma gondii dense granule antigen 7 (GRA7) against toxoplasmosis in mice using a prime-boost vaccination strategy. Vaccine 30(38):5631â5636
Minkoff H, Remington JS, Holman S, Ramirez R, Goodwin S, Landesman S (1997) Vertical transmission of toxoplasma by human immunodeficiency virus-infected women. Am J Obstet Gynecol 176(3):555â559
Mitchell CD, Erlich SS, Mastrucci MT, Hutto SC, Parks WP, Scott GB (1990) Congenital toxoplasmosis occurring in infants perinatally infected with human immunodeficiency virus 1. Pediatr Infect Dis J 9(7):512â518
Montoya JG, Liesenfeld O (2004) Toxoplasmosis. Lancet 363(9425):1965â1976
Montoya JG, Lowe KE, Clayberger C, Moody D, Do D, Remington JS et al (1996) Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect Immun 64(1):176â181
Mui EJ, Schiehser GA, Milhous WK, Hsu H, Roberts CW, Kirisits M et al (2008) Novel triazine JPC-2067-B inhibits Toxoplasma gondii in vitro and in vivo. PLoS Negl Trop Dis 2(3):e190
Neudeck A, Stachelhaus S, Nischik N, Striepen B, Reichmann G, Fischer HG (2002) Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7. Microbes Infect 4(6):581â590
Nielsen HV, LauemĂžller SL, Christiansen L, Buus S, Fomsgaard A, Petersen E (1999) Complete protection against lethal Toxoplasma gondii infection in mice immunized with a plasmid encoding the SAG1 gene. Infect Immun 67(12):6358â6363
Nguyen TD, Bigaignon G, Markine-Goriaynoff D, Heremans H, Nguyen TN, Warnier G et al (2003) Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and gamma-interferon. J Med Microbiol 52(Pt 10):869â876
Pappas G, Roussos N, Falagas ME (2009) Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis. Int J Parasitol 39(12):1385â1394
Paul M, Ma JK (2010) Plant-made immunogens and effective delivery strategies. Expert Rev Vaccines 9(8):821â833
Paul M, Ma JK (2011) Plant-made pharmaceuticals: leading products and production platforms. Biotechnol Appl Biochem 58(1):58â67
Pedersen MG, Stevens H, Pedersen CB, NĂžrgaard-Pedersen B, Mortensen PB (2011) Toxoplasma infection and later development of schizophrenia in mothers. Am J Psychiatry 168(8):814â821
Pelosi A, Shepherd R, Walmsley AM (2012) Delivery of plant-made vaccines and therapeutics. Biotechnol Adv 30(2):440â448
Petersen E, Nielsen HV, Christiansen L, Spenter J (1998) Immunization with E. coli produced recombinant T. gondii SAG1 with alum as adjuvant protect mice against lethal infection with Toxoplasma gondii. Vaccine 16(13):1283â1289
Petersen E, Vesco G, Villari S, Buffolano W (2010) What do we know about risk factors for infection in humans with Toxoplasma gondii and how can we prevent infections? Zoonoses Public Health 57(1):8â17
Pfrepper KI, Enders G, Gohl M, Krczal D, Hlobil H, Wassenberg D et al (2005) Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin Diagn Lab Immunol 12(8):977â982
Qu D, Wang S, Cai W, Du A (2008) Protective effect of a DNA vaccine delivered in attenuated Salmonella typhimurium against Toxoplasma gondii infection in mice. Vaccine 26(35):4541â4548
Quan JH, Chu JQ, Ismail HA, Zhou W, Jo EK, Cha GH, Lee YH (2012)Induction of protective immune responses by a multiantigenic DNA vaccine encoding GRA7 and ROP1 of Toxoplasma gondii. Clin Vaccine Immunol 19(5):666â674
Robert-Gangneux F, DardĂ© ML (2012) Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev 25(2):264â296
Saavedra R, Becerril MA, Dubeaux C, Lippens R, De Vos MJ, HĂ©rion P et al (1996) Epitopes recognized by human T lymphocytes in the ROP2 protein antigen of Toxoplasma gondii. Infect Immun 64(9):3858â3862
SĂĄnchez VR, Pitkowski MN, FernĂĄndez Cuppari AV, RodrĂguez FM, Fenoy IM, Frank FM et al (2011) Combination of CpG-oligodeoxynucleotides with recombinant ROP2 or GRA4 proteins induces protective immunity against Toxoplasma gondii infection. Exp Parasitol 128(4):448â453
Shang L, Liu Q, Liu W, Men J, Gao S, Jiang L et al (2009) Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against Toxoplasma gondii challenge. Vaccine 27(21):2741â2745
Stanford MR, Tan HK, Gilbert RE (2006) Toxoplasmic retinochoroiditis presenting in childhood: clinical findings in a UK survey. Br J Ophthalmol 90(12):1464â1467
Streatfield SJ (2007) Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnol J 5(1):2â15
Streatfield SJ, Howard JA (2003) Plant-based vaccines. Int J Parasitol 33(5â6):479â493
Subauste CS, Koniaris AH, Remington JS (1991) Murine CD8+ cytotoxic T lymphocytes lyse Toxoplasma gondii-infected cells. J Immunol 147(11):3955â3959
Tenter AM, Heckeroth AR, Weiss LM (2000) Toxoplasma gondii: from animals to humans. Int J Parasitol 30(12â13):1217â1258
Thomas F, Lafferty KD, Brodeur J, Elguero E, Gauthier-Clerc M, MissĂ© D (2012) Incidence of adult brain cancers is higher in countries where the protozoan parasite Toxoplasma gondii is common. Biol Lett 8(1):101â103
Velge-Roussel F, ChardĂšs T, MĂ©vĂ©lec P, Brillard M, Hoebeke J, Bout D (1994) Epitopic analysis of the Toxoplasma gondii major surface antigen SAG1. Mol Biochem Parasitol 66(1):31â38
Velge-Roussel F, Marcelo P, Lepage AC, Buzoni-Gatel D, Bout DT (2000) Intranasal immunization with Toxoplasma gondii SAG1 induces protective cells into both NALT and GALT compartments. Infect Immun 68(2):969â972
Vercammen M, Scorza T, Huygen K, De Braekeleer J, Diet R, Jacobs D et al (2000) DNA vaccination with genes encoding Toxoplasma gondii antigens RA1, GRA7, and ROP2 induces partially protective immunity against lethal challenge in mice. Infect Immun 68(1):38â45
Villena I, Ancelle T, Delmas C, Garcia P, Brezin AP, Thulliez P et al (2010) Congenital toxoplasmosis in France in 2007: first results from a national surveillance system. Euro Surveill 15(25)
Vittecoq M, Elguero E, Lafferty KD, Roche B, Brodeur J, Gauthier-Clerc M Thomas F et al (2012) Brain cancer mortality rates increase with Toxoplasma gondii seroprevalence in France. Infect Genet Evol 12(2):496â498
Wang H, He S, Yao Y, Cong H, Zhao H, Li T, Zhu XQ (2009) Toxoplasma gondii: protective effect of an intranasal SAG1 and MIC4 DNA vaccine in mice. Exp Parasitol 122(3):226â232
Weiss LM, Kim K (2000) The development and biology of bradyzoites of Toxoplasma gondii. Front Biosci 5:D391â405
Yolken RH, Dickerson FB, Fuller Torrey E (2009) Toxoplasma and schizophrenia. Parasite Immunol 31(11):706â715
Yusibov V, Streatfield SJ, Kushnir N (2011) Clinical development of plant-produced recombinant pharmaceuticals: vaccines, antibodies and beyond. Hum Vaccin 7(3):313â321
Zhang G, Huong VT, Battur B, Zhou J, Zhang H, Liao M, Kawase O, Lee EG, Dautu G, Igarashi M, Nishikawa Y, Xuan X (2007) A heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, induced protective immunity against Toxoplasma gondii infection in mice. Parasitology 134(Pt 10):1339â1346
Zhou H, Gu Q, Zhao Q, Zhang J, Cong H, Li Y et al (2007) Toxoplasma gondii: expression and characterization of a recombinant protein containing SAG1 and GRA2 in Pichia pastoris. Parasitol Res 100(4):829â835
Zucca M, Savoia D (2011) Current developments in the therapy of protozoan infections. Open Med Chem J 5:4â10
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this chapter
Cite this chapter
Clemente, M. (2014). Plant-Based Vaccines Against Toxoplasmosis. In: Rosales-Mendoza, S. (eds) Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0850-9_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0850-9_11
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-0849-3
Online ISBN: 978-1-4939-0850-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)