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Siderophore Efficacy of Fluorescent Pseudomonades Affecting Labeled Iron (59Fe) Uptake by Wheat (Triticum aestivum L.) Genotypes Differing in Fe Efficiency

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Use of Microbes for the Alleviation of Soil Stresses

Abstract

Plant growth promoting rhizobacteria (PGPR) may enhance iron (Fe) availability by producing some organic products including siderophores. Accordingly, the potential of fluorescent pseudomonades (selected from among 201 isolated strains) to produce auxin (IAA) and siderophore (Sid) and their effects on 59Fe uptake by two different wheat genotypes (Tabasi and Yavarous) were determined. High siderophore producing super-strains of Pseudomonas putida, P. fluorescens, and P. aeruginosa were isolated from wheat rhizosphere and their potential for siderophore production was evaluated by the chrome azurol-S assay (CAS blue agar). P. putida, the most efficient strain, produced a siderophore complex with 76 % efficiency compared with the standard siderophore, siderophore desferrioxamine B (DFOB). Bacterial siderophores significantly and differently affected the uptake of labeled 59Fe by wheat genotypes (Tabasi the more efficient cultivar) indicating that there may be differences in the chemical structure of the siderophores. Siderophore effectiveness on Fe availability was in the following order: Sid-DFOB> Sid-putida> Sid-fluorescens> Sid-areuginosa.

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Authors and Affiliations

  1. Department of Soil Science, Faculty of Agriculture, Urmia University, Urmia, Iran

    MirHassan Rasouli-Sadaghiani

  2. Department of Soil Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Mohammad J. Malakouti

  3. Department of Soil Microbiology, Soil and Water Research Institute (SWRI), Karaj, Iran

    Kazem Khavazi

  4. Department of Book and Article, AbtinBerkeh Limited Co, Malek Ave., Nazer Alley, #37, Isfahan, Iran

    Mohammad Miransari

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  1. MirHassan Rasouli-Sadaghiani
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  2. Mohammad J. Malakouti
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  3. Kazem Khavazi
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  4. Mohammad Miransari
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Correspondence to MirHassan Rasouli-Sadaghiani or Mohammad Miransari .

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  1. Department of Book&Article, AbtinBerkeh Limited Co., Isfahan, Iran

    Mohammad Miransari

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Rasouli-Sadaghiani, M., Malakouti, M.J., Khavazi, K., Miransari, M. (2014). Siderophore Efficacy of Fluorescent Pseudomonades Affecting Labeled Iron (59Fe) Uptake by Wheat (Triticum aestivum L.) Genotypes Differing in Fe Efficiency. In: Miransari, M. (eds) Use of Microbes for the Alleviation of Soil Stresses. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0721-2_7

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