Abstract
Tissue microarray (TMA) technology is a tool that allows for the rapid evaluation of a large number of tissue samples on a single section of a microscope slide [1]. Cores of paraffin embedded tissue are removed from the original donor block and re-embedded into a recipient block in an ordered pattern of hundreds of cores. The cores can be from a variety of normal and abnormal types of tissue or stages of tumor and can be used to survey marker expression. This is more expedient than staining and scoring full sections from each donor block; it conserves the reagents used in the assay (e.g., immunohistochemistry or fluorescent in situ hybridization) [1–9], and extends the use of the original donor blocks. Many techniques for preparing the TMAs are in use with variable performance characteristics depending upon the tissue type, the coring procedure, and instrumentation (Fig. 4.1). The advantage of TMA is that it allows for examination of markers with clinical features, associations to be made between different markers and of markers with response to therapy or prognosis, and assessment of markers for the purpose of guiding treatment.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsLiterature Cited
Kononen J, Bubendorf L, Kallioniemi A et al (1998) Tissue microarrays for high throughput molecular profiling of tumor specimens. Nat Med 4:844–847
Bubendorf L, Kolmer M, Kononen J et al (1999) Hormone therapy failure in human prostate cancer: analysis by complementary DNA and tissue microarrays. J Natl Cancer Inst 91:1758–1764
Moch H, Schraml P, Bubendorf L et al (1999) High-throughput tissue microarray analysis to evaluate genes uncovered by cDNA micrarray screening in renal cell carcinoma. Am J Pathol 154:981–986
Mucci NR, Akdas G, Manely S, Rubin MA (1999) Neuroendocrine expression in metastatic prostate cancer: evaluation of high-throughput tissue microarrays to detect heterogeneous protein expression. Hum Pathol 31:406–414
Perrone EE, Theoharis C, Mucci NR et al (2000) Tissue microarray assessment of prostate cancer tumor proliferation in African-American and white men. J Natl Cancer Inst 92:937–939
Schraml P, Kononen J, Bubendorf L et al (1999) Tissue microarrays for gene amplification surveys in many different tumor types. Clin Cancer Res 5:1966–1975
Lee CM, Lee RJ, Hammond E et al (2004) Expression of HER2neu (c-erbB-2) and epidermal growth factor receptor in cervical cancer: prognostic correlation with clinical characteristics, and comparison of manual and automated imaging analysis. Gynecol Oncol 93:209–214
Jensen T, Hammond E (2001) The tissue microarray—a technical guide for histologists. J Histotechnol 24(4):283–287
Jensen T (2003) Tissue microarray: advanced techniques. J Histotechnol 26(2):101–104
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this chapter
Cite this chapter
Russo, J., Sheriff, F., Pogash, T.J., Nguyen, T., Santucci-Pereira, J., Russo, I.H. (2014). Methodological Approach to Tissue Microarray for Studying the Normal and Cancerous Human Breast. In: Techniques and Methodological Approaches in Breast Cancer Research. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0718-2_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0718-2_4
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-0717-5
Online ISBN: 978-1-4939-0718-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)