Abstract
The present study was aimed to characterize 3-dimensional (3D) morphology of the cortical microvasculature (e.g., penetrating artery and emerging vein), using two-photon microscopy and automated analysis for their cross-sectional diameters and branching positions in the mouse cortex. We observed that both artery and vein had variable cross-sectional diameters across cortical depths. The mean diameter was similar for both artery (17 ± 5 μm) and vein (15 ± 5 μm), and there were no detectable differences over depths of 50–400 μm. On the other hand, the number of branches was slightly increased up to 400-μm depth for both the artery and vein. The mean number of branches per 0.1 mm vessel length was 1.7 ± 1.2 and 3.8 ± 1.6 for the artery and vein, respectively. This method allows for quantification of the large volume data of microvascular images captured with two-photon microscopy. This will contribute to the morphometric analysis of the cortical microvasculature in functioning brains.
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References
Zilles K, Wree A (1995) Cortex: areal and laminar structure. In: Paxinos G (ed) The rat nervous system, 2nd edn. Acadmic Press, San Diego, pp 649–685
Masamoto K, Tanishita K (2009) Oxygen transport in brain tissue. J Biomech Eng 131:7, 074002
Yoshihara K, Takuwa H, Kanno I, Okawa S, Yamada Y, Masamoto K (2013) 3D analysis of intracortical microvasculature during chronic hypoxia in mouse brains. Adv Exp Med Biol 765:357–363
Tomita Y, Kubis N, Calando Y, Tran Dinh A, Méric P, Seylaz J, Pinard E (2005) Long-term in vivo investigation of mouse cerebral microcirculation by fluorescence confocal microscopy in the area of focal ischemia. J Cereb Blood Flow Metab 25(7):858–867
Masamoto K, Tomita Y, Toriumi H, Aoki I, Unekawa M, Takuwa H, Itoh Y, Suzuki N, Kanno I (2012) Repeated longitudinal in vivo imaging of neuro-gliovascular unit at the peripheral boundary of ischemia in mouse cerebral cortex. Neuroscience 212:190–200
Sekiguchi Y, Masamoto K, Takuwa H, Kawaguchi H, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K (2013) Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse. Adv Exp Med Biol 789:419–425
Woolsey TA, Rovainen CM, Cox SB, Henegar MH, Liang GE, Liu D, Moskalenko YE, Sui J, Wei L (1996) Neuronal units linked to microvascular modules in cerebral cortex: response elements for imaging the brain. Cereb Cortex 6:647–660
Acknowledgments
This study was partially supported through funding from JSPS KAKENHI (#25750400) (to K.M.), JSPS KEKENHI (#24659578) (to I.K.), and a grant from the Ministry of Health, Labor and Welfare (MHLW), Japan (to I.K.).
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Sugashi, T. et al. (2014). Automated Image Analysis for Diameters and Branching Points of Cerebral Penetrating Arteries and Veins Captured with Two-Photon Microscopy. In: Swartz, H.M., Harrison, D.K., Bruley, D.F. (eds) Oxygen Transport to Tissue XXXVI. Advances in Experimental Medicine and Biology, vol 812. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0620-8_28
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DOI: https://doi.org/10.1007/978-1-4939-0620-8_28
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