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Antibody Microarrays for Cell-Based Assays: The Use of Micro-Arrayed Antibodies for Exploring Cell Surface Diversity or Whole Cell Functionality

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Cell-Based Microarrays

Part of the book series: SpringerBriefs in Cell Biology ((SBCB))

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Abstract

Since the early 1980s, microarrays have gained increasing interest due to their tremendous field of applications. A large repertoire of biomolecules has been micro-arrayed for the specific and parallelized detection of targets contained in a single biological sample. Most individual binding events target soluble compounds although pioneering microarray developments have been undertaken with whole cell binding on micro-arrayed glass slides. In this review, we wish to focus on micro-arrayed antibodies and their use for cell-based analysis. We will illustrate the wide range of applications that can be explored by site-specifically immobilizing eukaryotic or prokaryotic cells on a surface.

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Abbreviations

AML:

Acute myeloid leukemia

CCM:

Culture-capture-measure

CD:

Clusters of differentiation

CFU:

Colony forming units

CTC:

Circulating tumor cells

DNA:

Deoxynucleic acid

ELISA:

Enzyme-linked immunosorbent assay

FACS:

Fluorescence-assisted cell sorting

GNP:

Gold nanoparticles

HAART:

Highly active antiretroviral therapy

HIV:

Human immunodeficiency virus

ICS:

Intra-cellular cytokine staining

IFNγ:

Interferon gamma

IL:

Interleukin

IS:

Immunological synapses

MHC:

Major histocompatibility complex

mRNA:

Messenger ribonucleic acid

rRNA:

Ribosomal ribonucleic acid

LOD:

Limit of detection

PBMC:

Peripheral blood mononuclear cell

PCR:

Polymerase chain reaction

PDMS:

Polydimethylsiloxane

RLS:

Resonant light scattering

SECM:

Scanning electrochemical microscopy

SLE:

Systemic lupus erythematosus

SNP:

Single nucleotide polymorphisms

SPR:

Surface plasmon resonance

STEC:

Shiga toxin-producing E. coli

TCR:

T cell receptor

TNFα:

Tumor necrosis factor

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Authors and Affiliations

  1. SPrAM, UMR 5819 CEA/CNRS/UJF-Grenoble 1, Institute for Nanosciences and Cryogeny, CEA-Grenoble, 38054, Grenoble, France

    Yoann Roupioz Ph.D.

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Correspondence to Yoann Roupioz Ph.D. .

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© 2014 Ella Palmer

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Roupioz, Y. (2014). Antibody Microarrays for Cell-Based Assays: The Use of Micro-Arrayed Antibodies for Exploring Cell Surface Diversity or Whole Cell Functionality. In: Cell-Based Microarrays. SpringerBriefs in Cell Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0594-2_3

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