Summary
It is during ripening that the flavour and texture characteristic of the cheese variety develop. Three major pathways constitute the biochemistry of cheese ripening: (1) metabolism of residual lactose and of lactate and citrate, (2) lipolysis and fatty acid metabolism and (3) proteolysis and amino acid catabolism. Cheese ripening is mediated by metabolically active cells from the starter, non-starter and adjunct starter microbiotas and enzymes that contribute to ripening come from the coagulant (principally chymosin but sometimes other proteinases, and in cheeses made using rennet paste, also pregastric esterase), milk (plasmin, somatic cell enzymes and lipoprotein lipase), starter and non-starter lactic acid bacteria (cell envelope-associated proteinases and a wide range of intracellular peptidases, esterases and amino acid catabolic enzymes) and adjunct starters (proteinases, peptidases and lipases). The primary products of cheese ripening (peptides, amino acids and fatty acids) are metabolized further to volatile flavour compounds through metabolism of fatty acids and amino acid catabolism.
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References
Addeo F, Chianese L, Salzano A et al (1992) Characterization of the 12 % trichloroacetic acid insoluble oligopeptides of Parmigiano-Reggiano cheese. J Dairy Res 59:401–411
Addeo F, Chianese L, Sacchi R et al (1994) Characterization of the oligopeptides of Parmigiano-Reggiano cheese soluble in 120 g trichloroacetic acid/l. J Dairy Res 61:365–374
Alewijn M, Smit BA, Sliwinski EL et al (2007) The formation of lactones in Gouda cheese. Int Dairy J 17:59–66
Bastian ED, Brown RJ (1996) Plasmin in milk and dairy products: an update. Int Dairy J 6:435–457
Cantor MD, van den Tempel T, Hansen TK et al (2004) Blue cheese. In: Fox PF, McSweeney PLH, Cogan TM et al (eds) Cheese: Chemistry, Physics and Microbiology, Vol 2: Major Cheese Groups, 3rd edn. Elsevier Applied Science, Amsterdam, pp 175–198
Charton E, Davies C, McCrae AR (1992) Use of specific polyclonal antibodies to detect heterogeneous lipases from Geotrichum candidum. Biochim Biophys Acta 1127:191–198
Cogan TM, Hill C (1993) Cheese starter cultures. In: Fox PF (ed) Cheese: Chemistry, Physics and Microbiology, 2nd edn. Chapman & Hall, London, pp 193–255
Collins YF, McSweeney PLH, Wilkinson MG (2003) Lipolysis and free fatty acid catabolism in cheese: a review of current knowledge. Int Dairy J 13:841–866
Curtin ÁC, McSweeney PLH (2004) Catabolism of amino acids in cheese during ripening. In: Fox PF, McSweeney PLH, Cogan TM et al (eds) Cheese: Chemistry, Physics and Microbiology, Vol 1: General Aspects, 3rd edn. Elsevier Applied Science, Amsterdam, pp 436–454
Fox PF (1989) Proteolysis during cheese manufacture and ripening. J Dairy Sci 72:1379–1400
Fox PF, Kelly AL (2006a) Indigenous enzymes in milk: overview and historical aspects – Part 1. Int Dairy J 16:500–516
Fox PF, Kelly AL (2006b) Indigenous enzymes in milk: overview and historical aspects – Part 2. Int Dairy J 16:517–532
Fox PF, McSweeney PLH (1996) Proteolysis in cheese during ripening. Food Rev Int 12:457–509
Fox PF, McSweeney PLH (1998) Dairy Chemistry and Biochemistry. Chapman & Hall, London
Fox PF, Stepaniak L (1993) Enzymes in cheese technology. Int Dairy J 3:609
Fox PF, Wallace JM (1997) Formation of flavour compounds in cheese. Adv Appl Microbiol 45:17–85
Fox PF, Lucey JA, Cogan TM (1990) Glycolysis and related reactions during cheese manufacture and ripening. CRC Crit Rev Food Sci Nutr 29:237–253
Fox PF, Law J, McSweeney PLH et al (1993) Biochemistry of cheese ripening. In: Fox PF (ed) Cheese: Chemistry, Physics and Microbiology, vol 1, 2nd edn. Chapman & Hall, London, pp 389–438
Fox PF, Singh TK, McSweeney PLH (1994) Proteolysis in cheese during ripening. In: Andrews AT, Varley J (eds) Biochemistry of Milk Products. Royal Society of Chemistry, Cambridge, pp 1–31
Fox PF, McSweeney PLH, Singh TK (1995) Methods for assessing proteolysis in cheese during ripening. In: Malin EL, Tunick MH (eds) Chemistry of Structure-Function Relationships in Cheese. Plenum Press, New York, pp 161–194
Fox PF, O’Connor TP, McSweeney PLH et al (1996) Cheese: physical, biochemical and nutritional aspects. Adv Food Nutr Res 39:163–328
Fox PF, McSweeney PLH, Lynch CM (1998) Significance of non-starter lactic acid bacteria in Cheddar cheese. Aust J Dairy Technol 53:83–89
Gobbetti M, Fox PF, Stepaniak L (1996) Esterolytic and lipolytic activities of mesophilic and thermophilic lactobacilli. Italian J Food Sci 8:127–136
Grappin R, Rank TC, Olson NF (1985) Primary proteolysis of cheese proteins during ripening. J Dairy Sci 68:531–540
Gripon J-C (1987) Mould-ripened cheeses. In: Fox PF (ed) Cheese: Chemistry, Physics and Microbiology, vol 1. Elsevier Science Publishers, London, pp 121–149
Gripon J-C (1993) Mould-ripened cheeses. In: Fox PF (ed) Cheese: Chemistry, Physics and Microbiology, 2nd edn. Chapman & Hall, London, pp 111–136
Grufferty MB, Fox PF (1988) Milk alkaline proteinase: a review. J Dairy Res 55:609–630
Hemme D, Bouillanne C, Métro F et al (1982) Microbial catabolism of amino acids during cheese ripening. Sci Aliment 2:113–123
Holland R, Liu SQ, Crow VL et al (2005) Esterases of lactic acid bacteria and cheese flavour: milk fat hydrolysis, alcoholysis and esterification. Int Dairy J 15:711–718
Huffman LM, Kristoffersen T (1984) Role of lactose in Cheddar cheese manufacturing and ripening. N Z J Dairy Sci Technol 19:151–162
IDF (1991) Chemical Methods for Evaluation of Proteolysis in Cheese Maturation, Bulletin No. 261. International Dairy Federation, Brussels
Karahadian C, Lindsay RC (1987) Integrated roles of lactate, ammonia, and calcium in texture development of mold surface-ripening cheese. J Dairy Sci 70:909–918
Kelly AL, O’Flaherty F, Fox PF (2006) Indigenous proteolytic enzymes in milk: a brief overview of the present state of knowledge. Int Dairy J 16:563–572
Khalid NM, El-Soda M, Marth EH (1990) Esterase of Lactobacillus helveticus and Lactobacillus delbrueckii ssp. bulgaricus. J Dairy Sci 73:2711–2719
Kunji ERS, Mierau I, Hagting A et al (1996) The proteolytic system of lactic acid bacteria. Antonie Van Leeuwenhoek 70:187–221
Law BA (1987) Proteolysis in relation to normal and accelerated cheese ripening. In: Fox PF (ed) Cheese: Chemistry, Physics and Microbiology, vol 1. Elsevier Science Publishers, London, pp 365–392
Law J, Haandrikman A (1997) Proteolytic enzymes of lactic acid bacteria. Int Dairy J 7:1–11
Lenoir J (1984) The Surface Flora and its Role in the Ripening of Cheese, Bulletin No. 171. International Dairy Federation, Brussels, pp 3–20
Lowney SA (1997) Characterization of proteolysis in the Italian Smear-ripened cheese, Taleggio. MSc Thesis, National University of Ireland, Cork
McSweeney PLH (2004) Biochemistry of cheese ripening: introduction and overview. In: Fox PF, McSweeney PLH, Cogan TM et al (eds) Cheese: Chemistry, Physics and Microbiology, Vol 1: General Aspects, 3rd edn. Elsevier Applied Science, Amsterdam, pp 347–360
McSweeney PLH, Fox PF (1993) Cheese: methods of chemical analysis. In: Fox PF (ed) Cheese: Chemistry, Physics and Microbiology, vol 1. Elsevier Science Publishers, London, pp 341–388
McSweeney PLH, Fox PF (1997) Chemical methods for the characterization of proteolysis in cheese during ripening. Lait 77:41–76
McSweeney PLH, Fox PF (2004) Metabolism of residual lactose and of lactate and citrate. In: Fox PF, McSweeney PLH, Cogan TM et al (eds) Cheese: Chemistry, Physics and Microbiology, Vol 1: General Aspects, 3rd edn. Elsevier Applied Science, Amsterdam, pp 361–371
McSweeney PLH, Fox PF, Lucey JA et al (1993a) Contribution of the indigenous microflora to the maturation of Cheddar cheese. Int Dairy J 3:613–634
McSweeney PLH, Olson NF, Fox PF et al (1993b) Proteolytic specificity of chymosin on bovine αs1-casein. J Dairy Res 60:401–412
McSweeney PLH, Olson NF, Fox PF et al (1994) Proteolysis of bovine αs2-casein by chymosin. Z Lebensm-Unters Forsch 119:429–432
McSweeney PLH, Fox PF, Olson NF (1995) Proteolysis of bovine casein by cathepsin D: preliminary observations and comparison with chymosin. Int Dairy J 5:321–336
Monnet V, Chapot-Chartier MP, Gripon J-C (1993) Les peptidases des lactocoques. Lait 73:97–108
Mooney JS, Fox PF, Healy A et al (1998) Identification of the principal water-insoluble peptides in Cheddar cheese. Int Dairy J 8:813–818
Mottar JF (1989) Effect on the quality of dairy products. In: McKellar RC (ed) Enzymes of Psychrotrophs of Raw Foods. CRC Press, Boca Raton, FL, pp 227–243
Nelson JH, Jensen RG, Pitas RE (1977) Pregastric esterase and other oral lipases – a review. J Dairy Sci 60:327–362
O’Mahony JA, Fox PF, Kelly AL (2013) Indigenous enzymes of milk. In: McSweeney PLH, Fox PF (eds) Advanced Dairy Chemistry, Vol 1A. Proteins: Basic Aspects, 4th edn. Springer, New York, pp 337–385
Olivecrona T, Bengtsson-Olivecrona G (1991) Indigenous enzymes in milk: lipase. In: Fox PF (ed) Food Enzymology, vol 1. Elsevier Science Publishers, London, pp 62–78
Olivecrona T, Vilaro S, Bengtsson-Olivecrona G (1992) Indigenous enzymes in milk. II. Lipases in milk. In: Fox PF (ed) Advanced Dairy Chemistry, vol 1. Elsevier Science Publishers, London, pp 292–310
Oterholm A, Ordal ZJ, Witter LD (1970) Purificaiton and properties of glycerol ester hydrolase (lipase) from Propionibacterium shermanii. Appl Microbiol 20:16–22
Parente E, Cogan TM (2004) Starter cultures: general aspects. In: Fox PF, McSweeney PLH, Cogan TM et al (eds) Cheese: Chemistry, Physics and Microbiology, Vol 1: General Aspects, 3rd edn. Elsevier Applied Science, Amsterdam, pp 123–147
Rank TC, Grappin R, Olson NF (1985) Secondary proteolysis of cheese during ripening: a review. J Dairy Sci 68:801–805
Rattray FP, Fox PF (1997) Purification and characterization of an intracellular esterase from Brevibacterium linens ATCC 9174. Int Dairy J 7:273–278
Rattray FP, Fox PF (1998) Recently identified enzymes of Brevibacterium linens ATCC 9174 – a review. J Food Biochem 22:353–373
Rea DM (1997) Comparison of Cheddar cheese made with Chymosin, Rhizomucor miehei Proteinase or Chryphonectria parasitica Proteinases. MSc Thesis, National University of Ireland, Cork
Shakeel-ur-Rehman, Waldron, DS, Fox, PF (2004). Effect of modifying lactose concentration in cheese curd on proteolysis and quality of Cheddar cheese. Int Dairy J 14:591–597
Sidebottom CM, Charton E, Dunn PPJ et al (1991) Geotrichum candidum produces several lipases with markedly different substrate specificities. Eur J Biochem 202:485–491
Sousa MJ, Ardo Y, McSweeney PLH (2001) Advances in the study of proteolysis in cheese during ripening. Int Dairy J 11:327–345
Tan PST, Poolman B, Konings WN (1993) Proteolytic enzymes of Lactococcus lactis. J Dairy Res 60:269–286
Thomas TD (1987) Acetate production from lactate and citrate by non-starter bacteria in Cheddar cheese. N Z J Dairy Sci Technol 22:25–38
Thomas TD, Crow VL (1983) Mechanism of D(−)-lactic acid formation in Cheddar cheese. N Z J Dairy Sci Technol 18:131–141
Thomas TD, Pritchard GC (1987) Proteolytic enzymes in dairy starter cultures. FEMS Microbiol Rev 46:245–268
Tzanetakis N, Litopoulou-Tzanetaki E (1989) Biochemical activities of Pediococcus pentosaceus isolates of dairy origin. J Dairy Sci 72:859–863
Upadhyay VK, McSweeney PLH, Magboul AAA et al (2004) Proteolysis in cheese during ripening. In: Fox PF, McSweeney PLH, Cogan TM et al (eds) Cheese: Chemistry, Physics and Microbiology, Vol 1: General Aspects, 3rd edn. Elsevier Applied Science, Amsterdam, pp 392–433
Visser S (1993) Proteolytic enzymes and their relation to cheese ripening and flavor: an overview. J Dairy Sci 76:329–350
Waldron DS (1997) Effect of lactose concentration on the quality of Cheddar cheese. MSc Thesis, National University of Ireland, Cork
Woo AH, Lindsay RC (1984) Concentration of major free fatty acids and flavor development in Italian cheese varieties. J Dairy Sci 67:960–968
Woo AH, Kollodge S, Lindsay RC (1984) Quantitation of major free fatty acids in several cheese varieties. J Dairy Sci 67:874–878
Yvon M, Rijnen L (2001) Cheese flavour by amino acid catabolism. Int Dairy J 11:185–201
Zarmpoutis IV (1995) Proteolysis in Bllue-veined cheese varieties. MSc Thesis, National University of Ireland, Cork
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Fox, P.F., Guinee, T.P., Cogan, T.M., McSweeney, P.L.H. (2017). Biochemistry of Cheese Ripening. In: Fundamentals of Cheese Science. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-7681-9_12
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