Abstract
Several laboratories have successfully recovered mature plants from maize protoplasts [9, 11, 12, 15, 16]. In all cases, embryogenic suspension cultures were the source of competent protoplasts (those capable of division and plant regeneration). The limiting step has been to produce such suspensions repeatedly. Friable, embryogenic callus cultures were used as initial tissue for suspension cultures. The only commonality among the methods used to derive suspensions in these laboratories was that the donor suspensions seemed to require a minimum period (weeks to months) of growth in liquid culture before yielding competent protoplasts.
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© 1992 Springer Science+Business Media Dordrecht
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Rhodes, C.A., Gray, D.W. (1992). Transformation and regeneration of maize protoplasts. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3778-0_4
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DOI: https://doi.org/10.1007/978-1-4899-3778-0_4
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