Abstract
In vitro fertilization with single gametes has been performed using isolated egg cells from embryo sacs and sperm cells from mature pollen grains [12, 13]. Pairs of gametes have been fused individually by an electrical pulse after dielectrophoretical alignment under microscopic observation (Fig. 1, Fig. 2). In addition, fusion products have been created by fusion of single sperm cells with single synergids or central cells.
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© 1992 Springer Science+Business Media Dordrecht
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Kranz, E. (1992). In vitro fertilization of maize mediated by electrofusion of single gametes. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3776-6_3
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DOI: https://doi.org/10.1007/978-1-4899-3776-6_3
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