Summary
Ribosomal DNA (rDNA) in species of Talaromyces and related genera were examined in an initial attempt to understand their phylogenetic relationships. The variability in the nuclear rDNA repeat unit was studied by the restriction fragments of total DNA that hybridized to the rDNA repeat unit of Neurospora crassa (pMF2). Each fragment was treated as a taxonomic character with two states, present or absent. Pairwise comparisons of all taxa were used to produce a matrix of similarity coefficients which were subjected to UPGMA cluster analysis.
A comparison of 29 species with 21 having multiple isolates showed 14 with a majority of the isolates as closest neighbours. Talaromyces species with Paecilomyces anamorphic states cluster with Byssochlatnys and Thermoascus species having Paecilomyces anamorphic states and not with Talaromyces species having Penicillium anamorphs. The results also indicate that the strictly anamorphic Penicillium species are not mixed in with the holomorphic species, but with one exception are clustered in a group that is well separated from most of the Talaromyces species.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Anderson, J. B., Petsche, D. M. and Smith, M. L. 1986. Restriction polymorphisms in biological species of Armillaria mellea. Mycologia 79: 69–76.
Förster, H., Kinscherf, T. G., Leong, S. A. and Maxwell, D. P. 1988. Estimation of relatedness between Phytophthora species by analysis of mitochondrial DNA. Mycologia 80: 466–478.
Free, S. J., Rice, P. W. and Metzenberg, R. L. 1979. Arrangement of the genes coding for ribosomal ribonucleic acids in Neurospora crassa. Journal of Bacteriology 137: 1219–1226.
Gyllensten, U. B. and Erlich, H. A. 1988. Generation of single stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA DQ alpha locus. Proceedings of the National Acadamy of Science, USA 85: 7652–7656.
Hoopes, B. C. and McClure, W. R. 1981. Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Research 9: 5493–5505.
Jorgenson, R. A. and Cluster, P. D. 1988. Modes and tempos in the evolution of nuclear ribosomal DNA: new characters for evolutionary studies and new markers for genetic and population studies. Annals of the Missouri Botanical Garden 75: 1238–1247.
Lee, S. B., Milgroom, M. G. and Taylor, J. W. 1988. A rapid, high yield mini-prep method for isolation of total genomic DNA from fungi. Fungal Genetics Newsletter 35: 23–24.
Malloch, D. 1981. The Plectomycete centrum. In “Ascomycete Systematics: The Luttrellian Concept”. D. R. Reynolds, ed. Pp. 73–91. New York: Springer Verlag.
Malloch, D. 1985. The Trichocomaceae: relationships with other Ascomycetes. In Advances in Penicillium and Aspergillus Systematics. R. A. Samson and J. I. Pitt, eds. pp. 365–382. New York and London: Plenum Press.
Maniatis, T., Fritsch, E. F. and Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory; Cold Spring Harbor, New York.
Mullis, K. B. and Faloona, F. A. 1987. Specific synthesis of DNA in vitro via a polymerase catalysed chain reaction. Methods in Enzymology 155: 335–350.
Natvig, D. O., Jackson, D. A. and Taylor, J. W. 1987. A random fragment hybridization analysis of evolution in the genus Neurospora: the status of four-spored strains. Evolution 41: 1003–1021.
Pitt, J.I. 1979a. The Genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Academic Press; London.
Pitt, J. I. 1979b. Geosmithia gen. nov. for Penicillium lavendulum and related species. Canadian Journal of Botany 57: 2021–2030.
Pitt, J.I. 1988. A laboratory guide to common Penicillium species. 2nd ed. North Ryde, N.S.W.: CSIRO Division of Food Processing.
Reed, K. R. and Mann, D. A. 1985. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Research 13: 7207–7221.
Rholf, F. J. 1988. NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 1.4. Setauket, New York: Applied Biostatistics.
Rigby, P. W. J., Dieckmann, M., Rhodes, C. and Berg, P. 1977. Labeling deoxyribonuclec acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113: 237–251.
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A. and Arnheim, N. 1985. Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, N.Y. 230: 1350–1354.
Samson, R. A. 1974. Paecilomyces and some allied Hyphomycetes. Studies in Mycology, Baarn 6: 1–119.
Sokal, R. R. and Sneath, P.H.A. 1963. Principles of Numerical Taxonomy. Freeman; San Francisco.
Stolk, A. C. and Samson, R. A. 1971. Studies on Talaromyces and related genera. I. Hamigera gen. nov. and Byssochlamys. Persoonia 6: 341–357.
Stolk, A. C. and Samson, R. A. 1972. The Genus Talaromyces. Studies on Talaromyces and Related Genera II. Studies in Mycology, Baarn 2: 1–67.
Subramanian, C. V. 1979. Phialidic Hyphomycetes and their teleomorphs-an analysis. In “The Whole Fungus”. Vol. 1. W. B. Kendrick, ed. Pp. 125–151. Ottawa: National Museums of Canada.
Taylor, J. W. 1986. Fungal evolutionary biology and mitochondrial DNA. Experimental Mycology 10: 259–269.
Wrischnik, L. A., Higuchi, R. G., Stoneking, M., Erlich, H. A., Arnheim, N. and Wilson, A. C. 1987. Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA. Nucleic Acids Research 15: 529–542.
Zolan, M. E. and Pukkila, P. J. 1986. Inheritance of DNA methylation in Coprinus cinereus. Molecular and Cellular Biology 6: 195–200.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Taylor, J.W., Pitt, J.I., Hocking, A.D. (1990). Ribosomal DNA Restriction Studies of Talaromyces Species with Paecilomyces and Penicillium Anamorphs. In: Samson, R.A., Pitt, J.I. (eds) Modern Concepts in Penicillium and Aspergillus Classification. NATO ASI Series, vol 185. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3579-3_32
Download citation
DOI: https://doi.org/10.1007/978-1-4899-3579-3_32
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-3581-6
Online ISBN: 978-1-4899-3579-3
eBook Packages: Springer Book Archive