Summary
Several black Aspergillus isolates have been analyzed by biochemical and genetic tools to investigate whether such techniques are useful for rapid, objective identification. Western blots were used to screen for pectin lyase production and Southern blots to look for homology in these isolates with respect to their pectin lyase gene(s). All A. niger isolates can easily be identified by these methods. They produce a similar pectin lyase, although differences in molecular weigth and reactivity with a monoclonal antibody exist. Also all A. niger isolates contain at least one conserved gene: the pelD gene. There are other hybridizing bands visible, probably resulting from heterologous hybridization with another pectin lyase gene. Classification on the basis of presence of these bands is different from classification on the basis of morphological features. The other species of sect. Nigri differ in one or more of these aspects with the A. niger aggregate, except A. foetidus.
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van Someren, M.A.K., Kester, H.C.M., Samson, R.A., Visser, J. (1990). Variation in Pectinolytic Enzymes of the Black Aspergilli: A Biochemical and Genetic Approach. In: Samson, R.A., Pitt, J.I. (eds) Modern Concepts in Penicillium and Aspergillus Classification. NATO ASI Series, vol 185. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3579-3_29
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