Summary
The analysis of restriction fragment length polymorphisms (RFLP’s) in both nuclear and mitochondrial DNA is proving useful in establishing phylogenetic relationships in the section Nidulantes of Aspergillus. Largely because of the presence of a highly diagnostic perfect stage, this group has a firm and well established taxonomy, and the well developed genetics (making use of sexual, parasexual and protoplast fusion methods) has enabled the concept of the biological species to be applied. An examination of RFLP’s in the nuclear and mitochondrial DNA of these species has allowed a comparison to be made between the extent and nature of such variation in the DNA sequences with the group structure imposed by the various systematic studies and thus permits an interpretation of the RFLP’s in terms of that systematic classification. The highly diagnostic nature of restriction fragment variation in the mitochondrial genomes and of certain probes for detecting nuclear RFLP’s will be described.
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Croft, J.H., Bhattacherjee, V., Chapman, K.E. (1990). RFLP Analysis of Nuclear and Mitochondrial DNA and its Use in Aspergillus Systematics. In: Samson, R.A., Pitt, J.I. (eds) Modern Concepts in Penicillium and Aspergillus Classification. NATO ASI Series, vol 185. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3579-3_28
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