Abstract
Carbohydrates are a major class of structural components in bacterial cell envelopes. Sugar profiles can differentiate and identify isolated bacteria. Carbohydrates can also serve as chemical markers for direct detection of bacteria in complex matrices such as mammalian body fluids and tissues. This chapter reviews the composition of some bacterial polysaccharides and their characteristic sugars. Preparation and analysis of derivatives of these sugar monomers by gas chromatography (GC) and mass spectrometry (MS) is described. A modified alditol acetate procedure developed in our laboratories is presented along with some examples of its use.
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Fox, A., Gilbart, J., Morgan, S.L. (1990). Profiling and Detection of Bacterial Carbohydrates. In: Fox, A., Morgan, S.L., Larsson, L., Odham, G. (eds) Analytical Microbiology Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3564-9_5
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DOI: https://doi.org/10.1007/978-1-4899-3564-9_5
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