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Diagnosis and Molecular Biology of Tuberculosis

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AIDS and Respiratory Medicine

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Abstract

Preceding chapters of this book have made it clear that the pattern of tuberculosis has changed, both clinically and epidemiologically. These changes have created new challenges for the diagnosis of tuberculosis and these will be the focus of the present chapter.

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References

  • Aber, V.R., Allen, B.W., Mitchison, D.A. et al. (1980) Quality control in tuberculosis bacteriology. 1. Laboratory studies on isolated positive cultures and the efficiency of direct smear examination. Tubercle, 61, 123–33.

    Article  PubMed  CAS  Google Scholar 

  • Alland, D., Kalkut, G.E., Moss, A.R. et al. (1994) Transmission of tuberculosis in New York City — An analysis by DNA fingerprinting and conventional epidemiologic methods. N. Engl. J. Med., 330, 1710–16.

    Article  PubMed  CAS  Google Scholar 

  • Andersen, A.B., Thybo, S., Godfrey-Faussett, P. and Stoker, N.G. (1993) Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum. Eur. J. Clin. Microbiol. Infect. Dis., 12, 922–7.

    Article  PubMed  CAS  Google Scholar 

  • Araj, G.F., Fahmawi, B.H., Chugh, T.D. and Abusalim, M. (1993) Improved detection of mycobacterial antigens in clinical specimens by combined enzyme-linked immunosorbent assays. Diagn. Microbiol. Infect. Dis., 17, 119–27.

    Article  PubMed  CAS  Google Scholar 

  • Arruda, A., Bomfim, G., Knights, R. et al. (1993) Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cells. Science, 261, 1454–7.

    Article  PubMed  CAS  Google Scholar 

  • Banerjee, A., Dubnau, E., Quemard, A. et al. (1994) inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science,263, 227–30.

    Google Scholar 

  • Bem, C., Patil, P.S., Elliott, A.M. et al. (1993) The value of wide-needle aspiration in the diagnosis of tuberculous lymphadenitis in Africa. AIDS, 7, 1221–5.

    Article  PubMed  CAS  Google Scholar 

  • Brindle, R.J., Nunn, P.P., Githui, W. et al. (1993) Quantitative bacillary response to treatment in HIV-associated pulmonary tuberculosis. Am. Rev. Respir. Dis., 147, 958–61.

    Article  PubMed  CAS  Google Scholar 

  • Brisson-Noel, A., Gicquel, B., Lecossier, D. et al. (1989) Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples. Lancet, 11, 1069–71.

    Article  Google Scholar 

  • Chiang, C.S., Chiang, C.D., Lin, J.W. et al. (1994) Neopterin, soluble interleukin-2 receptor and adenosine deaminase levels in pleural effusions. Respiration, 61, 150–4.

    Article  PubMed  CAS  Google Scholar 

  • Chintu, C., Bhat, G., Luo, C. et al. (1993) Seroprevalence of HIV type 1 infection in Zambian children with TB. Pediatr. Infect. Dis., 12, 499–504.

    Article  CAS  Google Scholar 

  • Choy, E.H.S., Chiecobianchi, F., Panayi, G.S. and Kingsley, G.H. (1994) Synovial fluid lymphocyte proliferation to tuberculin protein product derivative — a novel way of diagnosing tuberculous arthritis. Clin. Exp. Rheumatol., 12, 187–90.

    PubMed  CAS  Google Scholar 

  • Clarridge, J.E., Shawar, R.M., Shinnick, T.M. and Plikaytis, B.B. (1993) Large-scale use of polymerase chain reaction for detection of Mycobacterium-tuberculosis in a routine mycobacteriology laboratory. J. Clin. Microbiol., 31, 2049–56.

    PubMed  Google Scholar 

  • Condos, R., McClune, A., Rom, W.N. and Schluger, N.W. (1996) Peripheral-blood-based PCR assay to identify patients with active pulmonary tuberculosis. Lancet, 347, 1082–5.

    Article  PubMed  CAS  Google Scholar 

  • Coronado, V.G., Becksague, C.M., Hutton, M.D. et al. (1993) Transmission of multidrug-resistant Mycobacterium-tuberculosis among persons with human immunodeficiency virus infection in an urban hospital–epidemiologic and restriction fragment length polymorphism analysis. J. Infect. Dis., 168, 1052–5.

    Article  PubMed  CAS  Google Scholar 

  • Cundall, D.B. (1986) The diagnosis of pulmonary tuberculosis in malnourished Kenyan children. Ann. Trop. Paediatr., 6, 249–55.

    PubMed  CAS  Google Scholar 

  • Daley, C.L., Small, P.M., Schecter, G.F. et al. (1992) An outbreak of tuberculosis with accelerated progression among persons infected with human immunodeficiency virus. N. Engl. J. Med., 326, 231–5.

    Article  PubMed  CAS  Google Scholar 

  • Das, S., Chan, S.L., Allen, B.W. et al. (1993) Application of DNA fingerprinting with IS986 to sequential mycobacterial isolates obtained from pulmonary tuberculosis patients in Hong Kong before, during and after short-course chemotherapy. Tuber. Lung Dis., 74, 47–51.

    Article  PubMed  CAS  Google Scholar 

  • de Gracia, J., Curull, V., Vidal, R. and Morell, F. (1988) Diagnostic value of bronchoalveolar lavage in suspected pulmonary tuberculosis. Chest, 93, 329–32.

    Article  PubMed  Google Scholar 

  • Diperri, G., Danzi, M., DeChecchi, G. et al. (1989) Nosocomial epidemic of active tuberculosis among HIV-infected patients. Lancet, 11, 1502–4.

    Google Scholar 

  • Drucker, E., Alcabes, P., Bosworth, W. and Sckell, B. (1994) Childhood tuberculosis in the Bronx, New York. Lancet, 343, 1482–5.

    Article  PubMed  CAS  Google Scholar 

  • Duncan, L.E., Elliott, A., Hayes, R.J. et al. (1995) Tuberculin sensitivity and HIV-1 status of patients attending a sexually transmitted diseases clinic in Lusaka, Zambia: a cross-sectional study. Trans. Roy. Soc. Trop. Med. Hyg., 89, 37–40.

    Article  PubMed  CAS  Google Scholar 

  • Eisenach, K.D., Sifford, M.D., Cave, M.D. et al. (1991) Detection of Mycobacterium-tuberculosis in sputum samples using a polymerase chain reaction. Am. Rev. Respir. Dis., 144, 1160–3.

    Article  PubMed  CAS  Google Scholar 

  • Elliott, A.M., Luo, N., Tembo, G. et al. (1990) Impact of HIV on tuberculosis in Zambia: a cross sectional study. Br. Med. J., 301, 412–15.

    Article  CAS  Google Scholar 

  • Fishman, J.A., Roth, R.S., Zanzot, E. et al. (1994) Use of induced sputum specimens for microbiologic diagnosis of infections due to organisms other than Pneumocystis carinii. J. Clin. Microbiol., 32, 131–4.

    CAS  Google Scholar 

  • Genewein, A., Telenti, A., Bernasconi, C. et al. (1993) Molecular approach to identifying route of transmission of tuberculosis in the community. Lancet, 342, 841–4.

    Article  PubMed  CAS  Google Scholar 

  • Gilks, C.F., Brindle, R.J., Otieno, L.S. et al. (1990) Extrapulmonary and disseminated tuberculosis in HIV-1 seropositive patients presenting to the acute medical services in Nairobi. AIDS, 4, 981–5.

    Article  PubMed  CAS  Google Scholar 

  • Githui, W.A., Nunn, P.P., Juma, E. et al. (1992) Cohort study of HIV infection in tuberculosis patients, Nairobi, Kenya: comparison of bacteriological results. Tuber. Lung Dis., 73, 203–9.

    Article  PubMed  CAS  Google Scholar 

  • Godfrey-Faussett, P., Mortimer, P., Jenkins, P.A. and Stoker, N.G. (1992) Evidence of transmission of tuberculosis by DNA fingerprinting. Br. Med. J., 305, 221–3.

    Article  CAS  Google Scholar 

  • Godfrey-Faussett, P., Stoker, N.G., Scott, J.A.G. et al. (1993) DNA fingerprints from Mycobacterium tuberculosis do not change during the development of rifampicin resistance. Tuber. Lung Dis., 74, 240–3.

    Article  PubMed  CAS  Google Scholar 

  • Graham, N.M.H., Nelson, K.E., Solomon, L. et al. (1992) Prevalence of tuberculin positivity and skin test anergy in HIV-1-seropositive and HIV1-seronegative intravenous drug users. JAMA, 267, 369–73.

    Article  PubMed  CAS  Google Scholar 

  • Guelar, A., Gatell, J.M., Verdejo, J. et al. (1993) A prospective study of the risk of tuberculosis among HIV-infected patients. AIDS, 7, 1345–9.

    Article  PubMed  CAS  Google Scholar 

  • Haas, W.H., Butler, W.R., Woodley, C.L. and Crawford, J.T. (1993) Mixed-linker polymerase chain reaction–a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex. J. Clin. Microbiol., 31, 1293–8.

    PubMed  CAS  Google Scholar 

  • Hermans, P.W.M., van Sollingen, D., Dale, J.W. et al. (1990) Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. J. Clin. Microbiol., 28, 2051–8.

    PubMed  CAS  Google Scholar 

  • Herz, A., Leichsenring, M., Felten, M. et al. (1994) The diagnosis of pulmonary tuberculosis by gas chromatographic detection of tuberculostearic acid using flame ionisation detectors. Eur. J. Clin. Invest., 24, 114–8.

    Article  PubMed  CAS  Google Scholar 

  • Honore, N. and Cole, S.T. (1994) Streptomycin resistance in Mycobacteria. Antimicrob. Agents Chemother., 38, 238–42.

    Article  PubMed  CAS  Google Scholar 

  • Hosp, M., Elliott, A., Raynes, J. et al. (1994) Neopterin beta-2-microglobulin and acute phase proteins in Zambian patients with tuberculosis. Pteridines, 4, 99–100.

    Google Scholar 

  • Jonas, V., Alden, M.J., Curry, J.I. et al. (1993) Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. J. Clin. Microbiol., 31, 2410–6.

    PubMed  CAS  Google Scholar 

  • Jones, B.E., Young, S.M.M., Antoniskis, D. et al. (1993) Relationship of the manifestations of tuberculosis to CD4 cell counts in patients with human immunodeficiency virus infection. Am. Rev. Respir. Dis., 148, 1292–7.

    Article  PubMed  CAS  Google Scholar 

  • Kent, R.J., Uttley, A.H.C., Stoker, N.G. et al. (1994) Evidence for nosocomial transmission of tuberculosis in an HIV care centre in the United Kingdom. Br. Med. J., 309, 639–40.

    Article  CAS  Google Scholar 

  • Khan, M.A., Kovnat, D.M., Bachus, B. et al. (1977) Clinical and roentgenographic spectrum of pulmonary tuberculosis in adults. Am. J. Med., 62, 31–8.

    Article  PubMed  CAS  Google Scholar 

  • Klein, R.S. and Motyl, M. (1993) Frequency of pulmonary tuberculosis in patients undergoing sputum induction for diagnosis of suspected Pneumocystis-carinii pneumonia. AIDS, 7, 135–15.

    Article  Google Scholar 

  • Kox, L.F.F., Rhienthong, D., Miranda, A.M. et al. (1994) More reliable PCR for detection of Mycobacterium tuberculosis in clinical samples. J. Clin. Microbiol., 32, 672–8.

    PubMed  CAS  Google Scholar 

  • Kwok, S. and Higuchi, R. (1989) Avoiding false positives with PCR. Nature, 339, 237–8.

    Article  PubMed  CAS  Google Scholar 

  • Levee, G., Glaziou, P., Gicquel, B. and Chanteau, S. (1994) Follow-up of tuberculosis patients undergoing standard anti-tuberculosis chemotherapy by using a polymerase chain reaction. Res. Microbiol., 145, 5–8.

    Article  PubMed  CAS  Google Scholar 

  • Lherminez, R.H. (1993) Urgent need for a new approach to the diagnosis of tuberculosis in developing countries in the decade of AIDS. Trop. Geogr. Med., 45, 145–9.

    CAS  Google Scholar 

  • Long, R., Scalcini, M., Manfreda, J. et al. (1991) The impact of HIV on the usefulness of sputum smears for the diagnosis of tuberculosis. Am. J. Public Health, 81, 1326–8.

    Article  PubMed  CAS  Google Scholar 

  • Longo, M.C., Berninger, M.S. and Hartley, J.L. (1990) The use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene, 93, 125–8.

    Article  PubMed  CAS  Google Scholar 

  • Lucas, S.B., Hounnou, A., Peacock, C. et al. (1993) The mortality and pathology of HIV infection in a West African City. AIDS, 7, 1569–79.

    Article  PubMed  CAS  Google Scholar 

  • Lucas, S.B., Peacock, C.S., Hounnou, A. et al. (1996) Diseases in children with and without HIV in Abidjan, Côte d’Ivoire: an autopsy study. Br. Med. J., 312, 335–8.

    Article  CAS  Google Scholar 

  • Mathur, P., Sacks, L., Auten, G. et al. (1994) Delayed diagnosis of pulmonary tuberculosis in city hospitals. Arch. Intern Med., 154, 306–10.

    Article  PubMed  CAS  Google Scholar 

  • McFadden, J. (ed.) (1990) Molecular Biology of the Mycobacteria. Academic Press, London.

    Google Scholar 

  • Miller, N., Hernandez, S.G. and Cleary, T.J. (1994) Evaluation of Gen-Probe amplified Mycobacterium tuberculosis direct test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens. J. Clin. Microbiol., 32, 393–7.

    PubMed  CAS  Google Scholar 

  • Mukadi, Y., Perriens, J.H., Stlouis, M.E. et al. (1993) Spectrum of immunodeficiency in HIV-1infected patients with pulmonary tuberculosis in Zaire Lancet, 342, 143–6.

    Article  PubMed  CAS  Google Scholar 

  • Nichols, L., Florentine, B., Lewis, W. et al. (1991) Bone marrow examination for the diagnosis of mycobacterial and fungal infections in the acquired immunodeficiency syndrome. Arch. Pathol. Lab. Med., 115, 1125–32.

    PubMed  CAS  Google Scholar 

  • Noordhoek, G.T., Kolk, A.H.J., Bjune, G. et al. (1994) Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis - a blind comparison study among seven laboratories. J. Clin. Microbiol., 32, 277–84.

    PubMed  CAS  Google Scholar 

  • Nunn, P. (1990) Impact of HIV–Discussion, in Tuberculosis: Back to the Future. (eds J.D.H. Porter and K.P.W.J. McAdam) John Wiley, Chichester, pp. 49–52.

    Google Scholar 

  • Pao, C.C., Lin. S.S., Wu, S.Y. et al. (1988) The detection of mycobacterial DNA sequences in uncultured clinical specimens with cloned Mycobacterium tuberculosis DNA as probes. Tubercle, 69, 27–36.

    Article  PubMed  CAS  Google Scholar 

  • Pastores, S.M., Naidich, D.P., Aranda, C.P. et al. (1993) Intrathoracic adenopathy associated with pulmonary tuberculosis in patients with human immunodeficiency virus infection. Chest, 103, 1433–7.

    Article  PubMed  CAS  Google Scholar 

  • Pearson, M.L., Jereb, J.A., Frieden, T.R. et al. (1992) Nosocomial transmission of multidrugresistant Mycobacterium tuberculosis: a risk to patients and health care workers. Ann. Intern. Med., 117, 191–6.

    Article  PubMed  CAS  Google Scholar 

  • Pedrobotet, J., Gutierrez, J., Miralles, R. et al. (1992) Pulmonary tuberculosis in HIV-infected patients with normal chest radiographs. AIDS, 6, 91–3.

    Article  CAS  Google Scholar 

  • Petterson, T., Klockars, M. and Weber, T. (1984) Pleural fluid adenosine deaminase in rheumatoid arthritis and systemic lupus erythematosus. Chest, 86, 273.

    Article  Google Scholar 

  • Pierre, C., Lecossier, D., Boussougant, Y. et al. (1991) Use of reamplification protocol improves sensitivity of detection of mycobacterium TB in clinical samples by amplification of DNA. J. Clin. Microbiol.,29, No. 4, 712–17.

    Google Scholar 

  • Pitchenik, A.E., Cole, C., Russell, B.W. et al. (1984) Tuberculosis, atypical mycobacteriosis, and AIDS among Haitian and non-Haitian patients in South Florida. Ann. Intern. Med., 101, 641–5.

    Article  PubMed  CAS  Google Scholar 

  • Pitchenik, A.E. and Robinson, H.A. (1985) The radiographic appearance of tuberculosis in patients with the acquired immunodeficiency syndrome. Am. Rev. Respir. Dis., 131, 393–6.

    PubMed  CAS  Google Scholar 

  • Radhakrishnan, V.V. and Mathai, A. (1991) A dot-immunobinding assay for the laboratory diagnosis of tuberculous meningitis and its comparison with enzyme-linked immunosorbent assay. J. Appli. Bacterial., 71, 428–33.

    Article  CAS  Google Scholar 

  • Raja, A., Machicao, A.R., Morrissey, A.B. et al. (1988) Specific detection of Mycobacterium tuberculosis in radiometric cultures by using an immunoassay for antigen 5. J. Infect. Dis., 158, 428–33.

    Article  Google Scholar 

  • Ross, B.C., Raios, K., Jackson, K. and Dwyer, B. (1992) Molecular cloning of a highly repeated DNA element from Mycobacterium-tuberculosis and its use as an epidemiological tool. J. Clin. Microbial., 30, 942–6.

    CAS  Google Scholar 

  • Rothel, J.S., Jones, S.L., Corner, L.A. et al. (1990) A sandwich enzyme immunoassay for bovine interferon-gamma and its use for the detection of tuberculosis in cattle. Aust. Vet. J, 67, 134–7.

    Article  PubMed  CAS  Google Scholar 

  • Saiki, R.K., Gelfand, D.H., Stoffel, S. et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487–91.

    Article  PubMed  CAS  Google Scholar 

  • Saltini, C., Amicosante, M., Girardi, E. et al. (1993) Early abnormalities of the antibody response against Mycobacterium-tuberculosis in human immunodeficiency virus infection. J. Infect. Dis., 168, 1409–14.

    Article  PubMed  CAS  Google Scholar 

  • Sanchez Hernandez, I.M., Pantoja, C. Ussetti, P. et al. (1991) Pleural fluid adenosine deaminase and lysozyme levels in the diagnosis of tuberculosis. Chest, 100, 1479–80.

    Article  Google Scholar 

  • Schluger, N.W., Kinney, D., Harkin, T.J. and Rom, W.N. (1994) Clinical utility of the polymerase chain reaction in the diagnosis of infections due to Mycobacterium tuberculosis. Chest, 105, 1116–21.

    CAS  Google Scholar 

  • Selwyn, P.A., Hartel, D., Lewis, V.A. et al. (1989) A prospective study of the risk of tuberculosis among intravenous drug users with human immunodeficiency virus infection. N. Engl. J. Med., 320, 545–50.

    Article  PubMed  CAS  Google Scholar 

  • Selwyn, P.A., Sckell, B.M., Alcabes, P. et al. (1992) High risk of active tuberculosis in HIV-infected drug users with cutaneous anergy. JAMA, 268, 504–8.

    Article  PubMed  CAS  Google Scholar 

  • Shafer, R.W., Goldberg, R., Sierra, M. and Glatt, A.E. (1989) Frequency of Mycobacterium tuberculosis bacteremia in patients with tuberculosis in an area endemic for AIDS. Am. Rev. Respir. Dis., 140, 1611–3.

    Article  PubMed  CAS  Google Scholar 

  • Shafer, R.W., Small, P.M., Larkin, C. et al. (1995) Temporal trends and transmission patterns during the emergence of multidrug resistant tuberculosis in New York City; a molecular epidiomologic assessment. J. Infect. Dis., 171, 170–6.

    Article  PubMed  CAS  Google Scholar 

  • Small, P.M., McClenny, N.B., Singh, S.P. et al. (1993) Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimise occurrence of false-positive cultures. J. Clin. Microbial., 31, 1677–82.

    CAS  Google Scholar 

  • Small, P.M., Hopewell, P.C., Singh, S.P. et al. (1994) The epidemiology of tuberculosis in San Francisco–A population-based study using conventional and molecular methods. N. Engl. J. Med., 330, 1703–9.

    Article  PubMed  CAS  Google Scholar 

  • Spargo, C.A., Haaland, P.D., Jurgensen, S.R. et al. (1993) Chemiluminescent detection of strand displacement amplified DNA from species comprising the Mycobacterium tuberculosis complex. Mol. Cell Probe., 7, 395–404.

    Article  CAS  Google Scholar 

  • Springett, V.H., Waaler, H.Th. and Nyboe, J. (1967) Results of the study on X-ray readings of the ad hoc committee for the study of classification and terminology in tuberculosis. Bull. Int. Union Tuber. Lung Dis., 17, 107–31.

    Google Scholar 

  • Starke, J.R., Taylor-Watts, K.T. (1989) Tuberculosis in the pediatric population of Houston, Texas. Pediatrics, 84, 28–35.

    PubMed  CAS  Google Scholar 

  • van Soolingen, D., Dehaas, P.E.W., Hermans, P.W.M. et al. (1993) Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J. Clin. Microbiol., 31, 1987–95.

    Google Scholar 

  • Telenti, M., Dequiros, J.F.B., Alvarez, M. et al. (1994) The diagnostic usefulness of DNA probe for Mycobacterium tuberculosis complex (Gen-Probe(R)) in BACTEC cultures versus other diagnostic methods. Infection, 22, 18–23.

    Article  PubMed  CAS  Google Scholar 

  • Telenti, A., Imboden, P., Marchesi, F. et al. (1993a) Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet, 341, 647–50.

    Article  CAS  Google Scholar 

  • Telenti, A., Imbodem P., Marchesi, F. et al. (1993b) Automated detection of rifampicin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis. Antimicrob. Agents Chemother., 37, 2054–8.

    Article  PubMed  CAS  Google Scholar 

  • van der Werf, T.S., Das, P.K., Van Soolingen, D. et al. (1992) Sero-diagnosis of tuberculosis with A60 antigen enzyme-linked immunosorbent assay: failure in HIV-infected individuals in Ghana. Med. Microbiol. Immunol., 181, 71–6.

    Article  PubMed  Google Scholar 

  • Verbon, A., Weverling, G.J., Kuijper, S. et al. (1993) Evaluation of different tests for the serodiagnosis of tuberculosis and the use of likelihood ratios in serology. Am. Rev. Respir. Dis., 148, 378–84.

    Article  PubMed  CAS  Google Scholar 

  • Walker, G.T., Little, M.C., Nadeau, J.G. and Shank, D.D. (1992a) Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc. Natl. Acad. Sci. USA, 89, 392–6.

    Article  PubMed  CAS  Google Scholar 

  • Walker, D.A., Taylor, I.K., Mitchell, D.M. and Shaw, R.J. (1992b) Comparison of polymerase chain reaction amplification of two mycobacterial DNA sequences, IS6110 and the 65 kDa antigen gene, in the diagnosis of tuberculosis. Thorax, 47, 690.

    Article  PubMed  CAS  Google Scholar 

  • Wilkins, E.G.L. and Ivanyi, J. (1990) Potential value of serology for diagnosis of extrapulmonary tuberculosis. Lancet, 336, 641–4.

    Article  PubMed  CAS  Google Scholar 

  • Wilson, S.M., McNerney, R., Nye, P.M. et al. (1993) Progress towards a simplified polymerase chain reaction and its application to the diagnosis of tuberculosis. J. Clin. Microbiol., 31, 776–82.

    PubMed  CAS  Google Scholar 

  • Winndeen, E.S., Batt, C.A. and Wiedmann, M. (1993) Non-radioactive detection of Mycobacterium-tuberculosis LCR products in a microtitre plate format. Mol. Cell Probe, 7, 179–86.

    Article  CAS  Google Scholar 

  • Yang, Z.H., Mtoni, I., Chonde, M. et al. (1995) DNA finger-printing and phenotyping of Mycobacterium tuberculosis isolates from HIVseropositive and HIV-seronegative patients in Tanzania. J. Clin. Microbiol., 33, 1064–9.

    PubMed  CAS  Google Scholar 

  • Zhang, Y., Heym, B., Allen, B. et al. (1992) The catalase-peroxidase gene and isoniazid resistance of Mycobacterium TB. Nature, 358, 591–3.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Peter Godfrey-Faussett
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Editors and Affiliations

  1. Department of Medicine, University College London Medical School, London, UK

    Alimuddin Zumla BSc MB ChB MSc PhD FRCP (Director, Visiting Professor, Honorary Consultant in Infectious Diseases) (Director, Visiting Professor, Honorary Consultant in Infectious Diseases)

  2. Camden and Islington Community Health Services NHS Trust, London, UK

    Alimuddin Zumla BSc MB ChB MSc PhD FRCP (Director, Visiting Professor, Honorary Consultant in Infectious Diseases) (Director, Visiting Professor, Honorary Consultant in Infectious Diseases)

  3. Department of Medicine, University of Zambia School of Medicine, Lusaka, Zambia

    Alimuddin Zumla BSc MB ChB MSc PhD FRCP (Director, Visiting Professor, Honorary Consultant in Infectious Diseases) (Director, Visiting Professor, Honorary Consultant in Infectious Diseases)

  4. Department of Respiratory Medicine, The Royal Free Hospital, London, UK

    Margaret Johnson MD, FRCP (Consultant Physician) (Consultant Physician)

  5. Department of Sexually Transmitted Diseases, Division of Pathology and Infectious Diseases, University College London Medical School, London, UK

    Robert Miller MB FRCP (Senior Lecturer/Honorary Consultant Physician, Honorary Consultant Physician) (Senior Lecturer/Honorary Consultant Physician, Honorary Consultant Physician)

  6. Camden and Islington Community Health Services NHS Trust, London, UK

    Robert Miller MB FRCP (Senior Lecturer/Honorary Consultant Physician, Honorary Consultant Physician) (Senior Lecturer/Honorary Consultant Physician, Honorary Consultant Physician)

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Godfrey-Faussett, P. (1997). Diagnosis and Molecular Biology of Tuberculosis. In: Zumla, A., Johnson, M., Miller, R. (eds) AIDS and Respiratory Medicine. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3446-8_11

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  • DOI: https://doi.org/10.1007/978-1-4899-3446-8_11

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