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Laboratory procedures and some of the analytical techniques used in vitamin determinations

  • Chapter
Bioavailability and Analysis of Vitamins in Foods
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Abstract

In the context of human and animal nutrition, vitamins are physiologically active substances. To assess the nutritional value of a food commodity or diet with respect to a particular vitamin it is necessary to determine the vitamin’s biological activity. Biological activity refers to the ability of a nutrient to fulfil a specific biological function or metabolic requirement. The only direct means of determining biological activity is a bioassay based on biological function. Such assays were the means by which the vitamins were originally recognized. They are still required today to measure the biopotency of new vitamin derivatives and food supplements, as well as validating new nonbiological assay methods.

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References

  • Ahuja, S. (1976) Derivatization in gas chromatography. J. Pharm. Sci., 65, 163–82.

    Article  CAS  Google Scholar 

  • Alcock, S.C., Finglas, P.M. and Morgan, M.R.A. (1992) Production and purification of an R-protein-enzyme conjugate for use in a microtitration plate protein-binding assay for vitamin B12 in fortified food. Food Chem., 45, 199–203.

    Article  CAS  Google Scholar 

  • Arella, F., Lahély, S., Bourguignon, J.B. and Hasselmann, C. (1996) Liquid chromatographic determination of vitamins B1 and B2 in foods. A collaborative study. Food Chem., 56, 81–6.

    Google Scholar 

  • Association of Vitamin Chemists, Inc. (1966) Methods of Vitamin Assay, 3rd edn, Interscience Publishers, New York, pp. 1–20.

    Google Scholar 

  • Ayi, B.K., Yuhas, D.A. and Deangelis, N.J. (1986) Simultaneous determination of vitamins B2 (riboflavin) and B6 (pyridoxine) in infant formula products by reverse phase liquid chromatography. J. Ass. Off. Analyt. Chem., 69, 56–9.

    CAS  Google Scholar 

  • Bakalyar, S.R. and Henry, R.A. (1976) Variables affecting precision and accuracy in high-performance liquid chromatography. J. Chromat., 126, 327–45.

    Article  CAS  Google Scholar 

  • Ball, G.F.M. (1988) Fat-Soluble Vitamin Assays in Food Analysis. Elsevier Applied Science, London.

    Google Scholar 

  • Barna, E. and Dworschâk, E. (1994) Determination of thiamine (vitamin B1) and riboflavin (vitamin B2) in meat and liver by high-performance liquid chromatography. J. Chromat., A., 668, 359–63.

    Article  CAS  Google Scholar 

  • Bartha, A., Vigh, G. and Varga-Puchony, Z. (1990) Basis of the rational selection of the hydrophobicity of the ion-pairing reagent in reversed-phase ion-pair high-performance liquid chromatography. J. Chromat., 499, 423–34.

    Article  CAS  Google Scholar 

  • Barton-Wright, E.C. (1952) The Microbiological Assay of the Vitamin B-Complex and Amino Acids, Sir Isaac Pitman & Sons, Ltd, London.

    Google Scholar 

  • Barton-Wright, E.C. (1967) The microbiological assay of certain vitamins in compound feeding stuffs. J. Ass. Publ. Analysts, 5, 8–23.

    CAS  Google Scholar 

  • Barton-Wright, E.W. (1971) The microbiological assay of the vitamin B6 complex (pyridoxine, pyridoxal and pyridoxamine) with Kloeckera brevis. Analyst, Lond., 96, 314–18.

    CAS  Google Scholar 

  • Bell, J.G. (1971) The microbiological assay of B-complex vitamins. In Proceedings of the University of Nottingham Residential Seminar on Vitamins (ed. M. Stein ), Churchill Livingstone, Edinburgh and London, pp. 165–87.

    Google Scholar 

  • Bell, J.G. (1974) Microbiological assay of vitamins of the B group in foodstuffs. Lab. Pract., 23, 235–42, 252.

    Google Scholar 

  • Betteridge, D. (1978) Flow injection analysis. Analyt. Chem., 50, 832A–3A, 835A–6A, 839A–40A, 842A - 4A, 846A.

    Google Scholar 

  • Bourgeois, C.F., George, P.R. and Cronenberger, L.A. (1984) Automated determination of a-tocopherol in food and feed. Part 2. Continuous flow technique. J. Ass. Off Analyt. Chem., 67, 631–4.

    CAS  Google Scholar 

  • Bui, M.H. (1987) Sample preparation and liquid chromatographic determination of vitamin D in food products. J. Ass. Off Analyt. Chem., 70, 802–5.

    CAS  Google Scholar 

  • Burton, D.E., Sepaniak, M.J. and Maskarinec, M.P. (1986) Analysis of B6 vitamers by micellar electrokinetic capillary chromatography with laser-excited fluorescence detection. J. Chromatogr. Sci., 24, 347–51.

    Article  CAS  Google Scholar 

  • Chase, G.W., Landen, W.O. Jr, Soliman, A.-G.M. and Eitenmiller, R.R. (1993) Method modification for liquid chromatographic determination of thiamine, riboflavin, and pyridoxine in medical foods. J. AOAC Int., 76, 1276–80.

    CAS  Google Scholar 

  • Chen, M. and McIntyre, P.A. (1979) Measurement of the trace amounts of vitamin B12 present in various foods by a new radiometric microbiologic technique. In Trace Organic Analysis: a New Frontier in Analytical Chemistry (eds H.S. Hertz and S.N. Chesler ), National Bureau of Standards, Washington, DC, pp. 257–65.

    Google Scholar 

  • Chen, M.F., Hill, J.W. and McIntyre, P.A. (1983) The folacin contents of foods as measured by a radiometric microbiologic method. J. Nutr., 113, 2192–6.

    CAS  Google Scholar 

  • Chiari, M., Nesi, M., Carrea, G. and Righetti, P.G. (1993) Determination of total vitamin C in fruits by capillary zone electrophoresis. J. Chromat., 645, 197–200.

    Article  CAS  Google Scholar 

  • Cooke, N.H.C. and Olsen, K. (1979) Chemically bonded alkyl reversed-phase columns. Am. Lab., 11, 45,46,48,50,52–5, 58–60.

    Google Scholar 

  • Cooke, N.H.C., Archer, B.G., Olsen, K. and Berick, A. (1982) Comparison of three-and five-micrometer column packings for reversed-phase liquid chromatography. Analyt. Chem., 54, 2277–83.

    Article  CAS  Google Scholar 

  • Dakshinamurti, K., Landman, A.D., Ramamurti, L. and Constable, R.J. (1974) Isotope dilution assay for biotin. Analyt. Biochem., 61, 225–31.

    Article  CAS  Google Scholar 

  • Davidek, J., Velisek, J., Cerna, J. and Davidek, T. (1985) Gas chromatographic determination of pantothenic acid in foodstuffs. J. Micronutr. Anal., 1, 39–46.

    CAS  Google Scholar 

  • Dawson, K.R., Unklesbay, N.F. and Hedrick, H.B. (1988) HPLC determination of riboflavin, niacin, and thiamin in beef, pork, and lamb after alternate heat-processing methods. J. Agric. Food Chem., 36, 1176–9.

    Article  CAS  Google Scholar 

  • Difco Laboratories (1985) Media for the microbiological assay of vitamins. In Difco Manual, 10th edn, Difco Laboratories, Detroit, MI, pp. 1055–1114.

    Google Scholar 

  • Dunbar, W.E. and Stevenson, K.E. (1979) Automated fluorometric determination of thiamine and riboflavin in infant formulas. J. Ass. Off. Analyt. Chem., 62, 642–7.

    CAS  Google Scholar 

  • Echols, R.E., Miller, R.H. and Foster, W. (1986) Analysis of thiamine in milk by gas chromatography and the nitrogen-phosphorus detector. J. Dairy Sci., 69, 1246–9.

    Article  CAS  Google Scholar 

  • Echols, R.E., Miller, R.H. and Thompson, L. (1985) Evaluation of internal standards and extraction solvents in the gas chromatographic determination of thiamine. J. Chromat., 347, 89–97.

    Article  CAS  Google Scholar 

  • Echols, R.E., Miller, R.H., Winzer, W. et al. (1983) Gas chromatographic determination of thiamine in meats, vegetables and cereals with a nitrogen-phosphorus detector. J. Chromat., 262, 257–63.

    Article  CAS  Google Scholar 

  • Elkins, E.R. and Dudek, J.A. (1985) Sampling for vitamin analysis. In Methods of Vitamin Assay, 4th edn (eds J. Augustin, B.P. Klein, D. Becker and P.B. Venugopal ), John Wiley & Sons, New York, pp. 135–51.

    Google Scholar 

  • Ewing, A.G., Wallingford, R.A. and Olefirowicz, T.M. (1989) Capillary electrophoresis. Analyt. Chem., 61, 292A–294A, 296A, 298A, 300A - 303A.

    Google Scholar 

  • Fellman, J.K., Artz, W.E., Tassinari, P.D. et al. (1982) Simultaneous determination of thiamin and riboflavin in selected foods by high-performance liquid chromatography. J. Food Sci., 47, 2048–50, 2067.

    Google Scholar 

  • Fernando, S.M. and Murphy, P.A. (1990) HPLC determination of thiamin and riboflavin in soybeans and tofu. J. Agric. Food Chem., 38, 163–7.

    Article  CAS  Google Scholar 

  • Finglas, P.M. and Faulks, R.M. (1984) The HPLC analysis of thiamin and riboflavin in potatoes. Food Chem., 15, 37–44.

    Article  CAS  Google Scholar 

  • Finglas, P.M., Faulks, R.M. and Morgan, M.R.A. (1986) The analysis of biotin in liver using a protein-binding assay. J. Micronutr. Anal., 2, 247–57.

    CAS  Google Scholar 

  • Finglas, P.M., Faulks, R.M. and Morgan, M.R.A. (1988) The development and characterisation of a protein-binding assay for the determination of folate–potential use in food analysis. J. Micronutr. Anal., 4, 295–308.

    CAS  Google Scholar 

  • Ford, J.E. (1953) The microbiological assay of ‘vitamin B12’. The specificity of the requirement of Ochromonas malhamensis for cyanocobalamin. Br. J. Nutr., 7, 299–306.

    Article  CAS  Google Scholar 

  • Froehlich, P. and Wehry, E.L. (1981) Fluorescence detection in liquid and gas chromatography. Techniques, examples, and prospects. In Modern Fluorescence Spectroscopy, Vol. 3 (ed. E.L. Wehry ), Plenum Press, New York, pp. 35–94.

    Chapter  Google Scholar 

  • Fujiwara, S., Iwase, S. and Honda, S. (1988) Analysis of water-soluble vitamins by micellar electrokinetic capillary chromatography. J. Chromat., 447, 133–40.

    CAS  Google Scholar 

  • Galfré, G. and Milstein, C. (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol., 73B, 3–46.

    Article  Google Scholar 

  • Garfield, F.M. (1989) Sampling in the analytical scheme. J. Ass. Off. Analyt. Chem., 72, 405–11.

    Google Scholar 

  • Gloor, R. and Johnson, E.L. (1977) Practical aspects of reverse phase ion pair chromatography. J. Chromatogr. Sci., 15, 413–23.

    Article  CAS  Google Scholar 

  • Goli, D.M. and Vanderslice, J.T. (1989) Microbiological assays of folacin using a CO2 analyzer system. J. Micronutr. Anal., 6, 19–33.

    CAS  Google Scholar 

  • Gould, B.J. (1988) The use of enzymes in ultrasensitive immunoassays. In Immunoassays for Veterinary and Food Analysis - 1 (eds B.A. Morris, M.N. Clifford and R. Jackman ), Elsevier Applied Science, London and New York, pp. 53–65.

    Google Scholar 

  • Guilarte, T.R. (1983) Radiometric microbiological assay of vitamin B6: assay simplification and sensitivity study. J. Ass. Off. Analyt. Chem., 66, 58–61.

    CAS  Google Scholar 

  • Guilarte, T.R. (1985) Analysis of biotin levels in selected foods using a radiometric-microbiological method. Nutr. Rep. Int., 32, 837–45.

    CAS  Google Scholar 

  • Guilarte, T.R. (1989) A radiometric microbiological assay for pantothenic acid in biological fluids. Analyt. Biochem., 178, 63–6.

    Article  CAS  Google Scholar 

  • Guilarte, T.R. (1991a) Radiometric microbiological assay of B vitamins. Part 1: assay procedure. J. Nutr. Biochem., 2, 334–8.

    Article  CAS  Google Scholar 

  • Guilarte, T.R. (1991b) Radiometric microbiological assay of B vitamins. Part 2: extraction methods. J. Nutr. Biochem., 2, 399–402.

    Article  CAS  Google Scholar 

  • Guilarte, T.R. and Pravlik, K. (1983) Radiometric-microbiologic assay of niacin using Kloeckera brevis: analysis of human blood and food. J. Nutr., 113, 2597–94.

    Google Scholar 

  • Hägg, M. (1994) Effect of various commercially available enzymes in the liquid chromatographic determination with external standardization of thiamine and riboflavin in foods. J. AOAC Int., 77, 681–6.

    Google Scholar 

  • Hamaker, H.C. (1986) A statistician’s approach to repeatability and reproducibility. J. Ass. Off. Analyt. Chem., 69, 417–28.

    Google Scholar 

  • Hasselmann, C., Franck, D., Grimm, P. et al. (1989) High-performance liquid chromatographic analysis of thiamin and riboflavin in dietetic foods. J. Micronutr. Anal., 5, 269–79.

    CAS  Google Scholar 

  • Hawker, C.D. (1973) Radioimmunoassay and related methods. Analyt. Chem., 45, 878A–882A, 884A, 886A, 888A, 890A.

    Google Scholar 

  • Hawthorne, S.B. (1990) Analytical-scale supercritical fluid extraction. Analyt. Chem., 62, 633A–636A, 638A - 642A.

    Google Scholar 

  • Healy, M., Jenkins, T. and Poliakoff, M. (1989) SFC: a hyphenated future. Lab. News, 7 August, 6–8.

    Google Scholar 

  • Hood, R.L. (1977) The use of linear regression analysis in the isotope dilution assay of biotin. Analyt. Biochem., 79, 635–8.

    Article  CAS  Google Scholar 

  • Hood, R.L. (1979) Isotopic dilution assay for biotin: use of [14C]biotin. Methods Enzymol., 62D, 279–83.

    Article  CAS  Google Scholar 

  • Horvath, C. and Melander, W. (1977) Liquid chromatography with hydrocarbonaceous bonded phases; theory and practice of reversed phase chromatography. J. Chromatogr. Sci., 15, 393–404.

    Article  CAS  Google Scholar 

  • Horwitz, W. (1990) Nomenclature for sampling in analytical chemistry (Recommendations 1990). Pure Appl. Chem., 62, 1193–208.

    Article  CAS  Google Scholar 

  • Ibanez, E., Herraiz, M. and Reglero, G. (1993) Use of micropacked columns for quantitative SFC. J. High Resolution Chromat., 16, 615–8.

    Article  CAS  Google Scholar 

  • Ibanez, E., Alvarez, P.J.M., Reglero, G. and Herraiz, M. (1993) Large particle micropacked columns in supercritical fluid chromatography. J. Microcol. Sep., 5, 371–81.

    Article  CAS  Google Scholar 

  • Ibanez, E., Tabera, J., Reglero, G. and Herraiz, M. (1995) Optimization of separation of fat-soluble vitamins by supercritical fluid chromatography using serial micropacked columns. J. Agric. Food Chem., 43, 2667–71.

    Article  CAS  Google Scholar 

  • Iskandarani, Z. and Pietrzyk, D.J. (1982) Ion interaction chromatography of organic anions on a poly(styrene-divinylbenzene) adsorbent in the presence of tetraalkylammonium salts. Analyt. Chem., 54, 1065–71.

    Article  CAS  Google Scholar 

  • Jorgenson, J.W. and Lukacs, K.D. (1983) Capillary zone electrophoresis. Science, 222, 266–72.

    Article  CAS  Google Scholar 

  • Kamman, J.F., Labuza, T.P. and Warthesen, J.J. (1980) Thiamin and riboflavin analysis by high performance liquid chromatography. J. Food Sci., 45, 1497–9, 1504.

    Article  Google Scholar 

  • Kmostak, S. and Kurtz, D.A. (1993) Rapid determination of supplemental vitamin E acetate in feed premixes by capillary gas chromatography, J. AOAC Int., 76, 735–41.

    CAS  Google Scholar 

  • Kneifel, W., Ulberth, F. and Winkler-Macheiner, U. (1987) HPLC methods for the simultaneous determination of retinol and tocopherol in butter and whole-milk powder. Deutsche Lebensm. Rundschau, 83, 137–9 (in German).

    CAS  Google Scholar 

  • Kobayashi, T., Okano, T. and Takeuchi, A. (1986) The determination of vitamin D in foods and feeds using high-performance liquid chromatography. J. Micronutr. Anal., 2, 1–24.

    CAS  Google Scholar 

  • Kratochvil, B. and Taylor, J.K. (1981) Sampling for chemical analysis. Analyt. Chem., 53, 924A–6A, 928A, 930A, 932A, 934A, 936A, 938A.

    Google Scholar 

  • Landen, W.O., Jr (1980) Application of gel permeation chromatography and nonaqueous reverse phase chromatography to high pressure liquid chromatographic determination of retinyl palmitate in fortified breakfast cereals. J. Ass. Off Analyt. Chem., 63, 131–6.

    CAS  Google Scholar 

  • Landen, W.O., Jr (1982) Application of gel permeation chromatography and nonaqueous reversed-phase chromatography to high performance liquid chromatographic determination of retinyl and a-tocopheryl acetate in infant formulas. J. Ass. Off. Analyt. Chem., 65, 810–16.

    CAS  Google Scholar 

  • Landen, W.O., Jr (1985) Liquid chromatographic determination of vitamins D2 and D3 in fortified milk and infant formulas. J. Ass. Off. Analyt. Chem., 68,183–7. Landen, W.O., Jr and Eitenmiller, R.R. (1979) Application of gel permeation chromatography and nonaqueous reverse phase chromatography to high pressure liquid chromatographic determination of retinyl palmitate and [3-carotene in oil and margarine. J. Ass. Off. Analyt. Chem., 62, 283–9.

    Google Scholar 

  • Lau, K.-S., Gottlieb, C., Wasserman, L.R. and Herbert, V. (1965) Measurement of serum vitamin Biz level using radioisotope dilution and coated charcoal. Blood, 26, 202–14.

    CAS  Google Scholar 

  • Lee, D.P. (1982) Reversed-phase HPLC from pH 1 to 13. J. Chromatogr. Sci., 20, 203–8.

    Article  Google Scholar 

  • Lee, H.A., Mills, E.N.C., Finglas, P.M. and Morgan, M.R.A. (1990) Rapid biospecific methods of vitamin analysis. J. Micronutr. Anal., 7, 261–70.

    CAS  Google Scholar 

  • Lim, K.L., Young, R.W., Palmer, J.K. and Driskell, J.A. (1982) Quantitative separation of B6 vitamers in selected foods by a gas—liquid chromatographic system equipped with an electron-capture detector. J. Chromat., 250, 86–9.

    Article  CAS  Google Scholar 

  • Lindeberg, J. (1996) Capillary electrophoresis in food analysis. Food Chem., 55, 73–94.

    Article  CAS  Google Scholar 

  • Majors, R.E. (1980a) Multidimensional high performance liquid chromatography. J. Chromatogr. Sci., 18, 571–9.

    Article  CAS  Google Scholar 

  • Majors, R.E. (1980b) Practical operation of bonded-phase columns in high-performance liquid chromatography. In High-performance Liquid Chromatography, Advances and Perspectives, Vol. 1 (ed. C. Horvath ), Academic Press, New York, pp. 75–111.

    Google Scholar 

  • Majors, R.E. (1986) Sample preparation for HPLC and gas chromatography using solid-phase extraction. Liquid Chromat.—Gas Chromat Mag., 4(10), 972, 980, 982, 984.

    Google Scholar 

  • Majors, R.E. (1991) Supercritical fluid extraction — an introduction. Liquid Chromat. Gas Chromat. Int., 4(3), 11–12, 14, 16–17.

    Google Scholar 

  • Marks, C. (1988) Determination of free tocopherols in deodorizer distillate by capillary gas chromatography. J. Am. Oil Chem. Soc., 65, 1936–9.

    Article  CAS  Google Scholar 

  • Marsili, R. and Callahan, D. (1993) Comparison of a liquid solvent extraction technique and supercritical fluid extraction for the determination of a-and [3- carotene in vegetables. J. Chromatogr. Sci., 31, 422–8.

    Article  CAS  Google Scholar 

  • Mauro, D.J. and Wetzel, D.L. (1984) Simultaneous determination of thiamine and riboflavin in enriched cereal based products by high-performance liquid chromatography using selective detection. J. Chromat., 299, 281–7.

    Article  CAS  Google Scholar 

  • Nishi, H., Tsumagari, N., Kakimoto, T. and Terabe, S. (1989) Separation of water-soluble vitamins by micellar electrokinetic chromatography. J. Chromat., 465, 331–43.

    Article  CAS  Google Scholar 

  • Olechno, J.D., Tso, J.M.Y., Thayer, J. and Wainright, A. (1990) Capillary electrophoresis: a multifaceted technique for analytical chemistry. Part 1. Separations. Am. Lab., 22, 51,52, 54, 55–9.

    Google Scholar 

  • Ollilainen, V., Vahteristo, L., Uusi-Rauva, A. et al. (1993) The HPLC determination of total thiamin (vitamin Bi) in foods. J. Food Comp. Anal., 516, 152–65.

    Article  Google Scholar 

  • Osborne, B.G. and Tyson, J.F. (1988) Review: flow injection analysis — a new technique for food and beverage analysis. Int. J. Food Sci. Technol., 23, 541–54.

    Article  CAS  Google Scholar 

  • Panfili, G., Manzi, P. and Pizzoferrato, L. (1994) High-performance liquid chromatographic method for the simultaneous determination of tocopherols, carotenes, and retinol and its geometric isomers in Italian cheeses. Analyst, Lond., 119, 1161–5.

    CAS  Google Scholar 

  • Pearson, W.N. (1967) Principles of microbiological assay. In The Vitamins. Chemistry, Physiology, Pathology, Methods, 2nd edn, Vol. VII (eds P. György and W.N. Pearson ), Academic Press, New York, pp. 1–26.

    Google Scholar 

  • Pomeranz, Y. and Meloan, C.E. (1978) Food Analysis: Theory and Practice, AVI Pub. Co. Inc., Westport, CT., pp. 11–20.

    Google Scholar 

  • Rabel, F.M. (1980) Use and maintenance of microparticle high performance liquid chromatography columns. J. Chromatogr. Sci., 18, 394–408.

    Article  CAS  Google Scholar 

  • Rabel, F.M. (1985) Instrumentation for small-bore liquid chromatography. J. Chromatogr. Sci., 23, 247–52.

    Article  CAS  Google Scholar 

  • Ranger, C.B. (1981) Flow injection analysis. Principles, techniques, application, design. Analyt. Chem., 53, 20A–22A, 24A, 26A, 28A, 30A, 32A.

    Google Scholar 

  • Rees, D.I. (1989) Determination of nicotinamide and pyridoxine in fortified food products by HPLC. J. Micronutr. Anal., 5, 53–61.

    CAS  Google Scholar 

  • Reyes, E.S.P. and Subryan, L. (1989) An improved method of simultaneous HPLC assay of riboflavin and thiamin in selected cereal products. J. Food Comp. Anal., 2, 41–7.

    Article  CAS  Google Scholar 

  • Reynolds, S.L. (1985) The use of HPLC in the determination of fat-soluble vitamins in a variety of milk-based food products. Proc. Inst. Food Sci. Technol. (UK), 18, 43–50.

    Google Scholar 

  • Reynolds, S.L. and Judd, H.J. (1984) Rapid procedure for the determination of vitamins A and D in fortified skimmed milk powder using high-performance liquid chromatography. Analyst, Lond., 109, 489–92.

    CAS  Google Scholar 

  • Rizzolo, A. and Polesello, S. (1992) Review. Chromatographic determination of vitamins in foods. J. Chromat., 624, 103–52.

    Article  CAS  Google Scholar 

  • Roy, R.B. (1979) Application of Technicon AutoAnalyzer II to the analysis of water-soluble vitamins in foodstuffs. In Topics in Automated Chemical Analysis, Vol. I, Technicon Ind. Systems, Tarrytown, New York, pp. 138–62.

    Google Scholar 

  • Roy, R.B. and Conetta, A. (1976) Automated analysis of water-soluble vitamins in food. Food Technol., 30(10), 94, 95, 98, 100, 103, 104.

    Google Scholar 

  • Russell, L.F. and Vanderslice, J.T. (1992) Comments on the standard fluorometric determination of riboflavin in foods and biological tissues. Food Chem., 43, 79–82.

    Article  CAS  Google Scholar 

  • Saito, M., Yamauchi, Y., Inomata, K. and Kottkamp, W. (1989) Enrichment of tocopherols in wheat germ by directly coupled supercritical fluid extraction with semipreparative supercritical fluid chromatography. J. Chromatogr. Sci., 27, 79–85.

    Article  CAS  Google Scholar 

  • Schneiderman, M.A., Sharma, A.K., Mahanama, K.R.R. and Locke, D.C. (1988) Determination of vitamin K1 in powdered infant formulas, using supercritical fluid extraction and liquid chromatography with electrochemical detection. J. Ass. Off. Analyt. Chem., 71, 815–7.

    CAS  Google Scholar 

  • Shearer, M.J. (1983) High-performance liquid chromatography of K vitamins and their antagonists. Adv. Chromat., 21, 243–301.

    CAS  Google Scholar 

  • Sims, A. and Shoemaker, D. (1993) Simultaneous liquid chromatographic determination of thiamine and riboflavin in selected foods. J. AOAC Int., 76, 1156–60.

    CAS  Google Scholar 

  • Slover, H.T., Thompson, R.H. Jr, Davis, C.S. and Merola, G.V. (1985) Lipids in margarines and margarine-like foods. J. Am. Oil Chem. Soc., 62, 775–86.

    Article  CAS  Google Scholar 

  • Snell, E.E. (1948) Use of microorganisms for assay of vitamins. Physiol. Rev., 28, 255–82.

    CAS  Google Scholar 

  • Snell, E.E. (1950) Microbiological methods in vitamin research. In Vitamin Methods, Vol. I (ed. P. György ), Academic Press, New York, pp. 327–505.

    Google Scholar 

  • Snyder, J.M., Taylor, S.L. and King, J.W. (1993) Analysis of tocopherols by capillary supercritical fluid chromatography and mass spectrometry. J. Am. Oil Chem. Soc., 70, 349–54.

    Article  CAS  Google Scholar 

  • Snyder, L., Levine, J., Stoy, R. and Conetta, A. (1976) Automated chemical analysis: update on continuous-flow approach. Analyt. Chem., 48, 942A–944A, 946A, 948A, 950A, 952A, 954A, 956A.

    Google Scholar 

  • Spanos, G.A., Chen, H. and Schwartz, S.J. (1993) Supercritical CO2 extraction of 0-carotene from sweet potatoes. J. Food Sci., 58, 817–20.

    Article  CAS  Google Scholar 

  • Stancher, B. and Zonta, F. (1983) HPLC of fat-soluble vitamins in cheese. New method for determining the total biological activity of vitamins A and E. Riv. Ital. Sostanze Grasse, 60, 371–5.

    CAS  Google Scholar 

  • Stranahan, J.J. and Deming, S.N. (1982) Thermodynamic model for reversed-phase ion-pair liquid chromatography. Analyt. Chem., 54, 2251–6.

    Article  CAS  Google Scholar 

  • Strohecker, R. and Henning, H.M. (1966) Vitamin Assay. Tested Methods, Verlag Chemie, Weinheim.

    Google Scholar 

  • Sullivan, D.M. and Carpenter, D.E. (eds) (1993) Methods of Analysis for Nutrition Labeling, AOAC International, Arlington, VA.

    Google Scholar 

  • Tanaka, A., lijima, M., Kikuchi, Y. et al. (1989) Gas chromatographic determination of nicotinamide in meats and meat products as 3-cyanopyridine. J. Chromat., 466, 307–17.

    Article  CAS  Google Scholar 

  • Tarli, P., Benocci, S. and Neri, P. (1971) Gas-chromatographic determination of pantothenates and panthenol in pharmaceutical preparations by pantoyl lac-tone. Analyt. Biochem., 42, 8–13.

    Article  CAS  Google Scholar 

  • Taylor, J.K. (1986) Role of collaborative and cooperative studies in evaluation of analytical methods. J. Ass. Off. Analyt. Chem., 69, 398–400.

    Google Scholar 

  • Thompson, J.N. and Madère, R. (1978) Automated fluorometric determination of vitamin A in milk. J. Ass. Off. Analyt. Chem., 61, 1370–3.

    CAS  Google Scholar 

  • Ulberth, F. (1991) Simultaneous determination of vitamin E isomers and cholesterol by GLC. J. High Resolut. Chromat., 14, 343–4.

    Article  CAS  Google Scholar 

  • Velisek, J. and Davidek, J. (1986) Gas—liquid chromatography of vitamins in foods: the water-soluble vitamins. J. Micronutr. Anal., 2, 25–42.

    CAS  Google Scholar 

  • Velisek, J., Davidek, J., Mnukovâ, J. and Pistèk, T. (1986) Gas chromatographic determination of thiamin in foods. J. Micronutr. Anal., 2, 73–80.

    CAS  Google Scholar 

  • Vivilecchia, R.V., Lightbody, B.G., Thimot, N.Z. and Quinn, H.M. (1977) The use of microparticulates in gel permeation chromatography. J. Chromatogr. Sci., 15, 424–33.

    Article  CAS  Google Scholar 

  • Voigt, M.N., Eitenmiller, R.R. and Ware, G.O. (1979) Vitamin analysis by microbial and protozoan organisms: response to food preservatives and neutralization salts. J. Food Sci., 44, 723–8, 737.

    Google Scholar 

  • Walsh, J.H., Wyse, B.W. and Hansen, R.G. (1979) A comparison of microbiological and radioimmunoassay methods for the determination of pantothenic acid in foods. J. Food Biochem., 3, 175–89.

    Article  Google Scholar 

  • Waxman, S., Schreiber, C. and Herbert, V. (1971) Radioisotopic assay for measurement of serum folate levels. Blood, 38, 219–28.

    CAS  Google Scholar 

  • Wehling, R.L. and Wetzel, D.L. (1984) Simultaneous determination of pyridoxine, riboflavin, and thiamin in fortified cereal products by high-performance liquid chromatography. J. Agric. Food Chem., 32, 1326–31.

    Article  CAS  Google Scholar 

  • White, C.M., Gere, D.R., Boyer, D. et al. (1988) Analysis of pharmaceuticals and other solutes of biochemical importance by supercritical fluid chromatography. J. High Resolution Chromat. Chromatogr. Commun., 11, 94–8.

    Article  CAS  Google Scholar 

  • Wickroski, A.F. and McLean, L.A. (1984) Improved reverse phase liquid chromatographic determination of vitamins A and D in fortified milk. J. Ass. Off. Analyt. Chem., 67, 62–5.

    CAS  Google Scholar 

  • Widicus, W.A. and Kirk, J.R. (1979) High performance liquid chromatographic determination of vitamins A and E in cereal products. J. Ass. Off. Analyt. Chem., 62, 637–41.

    CAS  Google Scholar 

  • Wiedemer, R.T., McKinley, S.L. and Rendl, T.W. (1986) Advantages of wide-bore capillary columns. Int. Lab., 16, May, 68, 70, 72, 74, 76, 77.

    Google Scholar 

  • Williams, W.L., Broquist, H.P. and Snell, E.E. (1947) Oleic acid and related compounds as growth factors for lactic acid bacteria. J. Biol. Chem., 170, 619–30.

    CAS  Google Scholar 

  • Wills, R.B.H., Wimalasiri, P. and Greenfield, H. (1985) Comparative determination of thiamin and riboflavin in foods by high-performance liquid chromatography and fluorometric methods. J. Micronutr. Anal., 1, 23–9.

    CAS  Google Scholar 

  • Wimalasiri, P. and Wills, R.B.H. (1985) Simultaneous analysis of thiamin and riboflavin in foods by high-performance liquid chromatography. J. Chromat., 318, 412–16.

    Article  CAS  Google Scholar 

  • Woodrow, I.L., Torrie, K.M. and Henderson, G.A. (1969) A rapid method for the determination of riboflavin in dried milk products. J. Inst. Can. Technol. Aliment., 2, 120–2.

    CAS  Google Scholar 

  • Yik, Y.F., Lee, H.K., Li, S.F.Y. and Khoo, S.B. (1991) Micellar electrokinetic capillary chromatography of vitamin B6 with electrochemical detection. J. Chromat., 585, 139–44.

    Article  CAS  Google Scholar 

  • Zamarreño, M.M.D., Pérez, A.S., Pérez, C.G. and Méndez, J.H. (1992) High-performance liquid chromatography with electrochemical detection for the simultaneous determination of vitamin A, D3 and E in milk. J. Chromat., 623, 69–74.

    Article  Google Scholar 

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© 1998 G.F.M. Ball

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Ball, G.F.M. (1998). Laboratory procedures and some of the analytical techniques used in vitamin determinations. In: Bioavailability and Analysis of Vitamins in Foods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3414-7_2

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