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Discovery and bacteriology

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Abstract

It has been quite a shock to the system to discover that several of the gastroduodenal diseases that we thought we understood fairly well are actually caused by a bacterium; Helicobacter pylori. This book aims to explore the clinical implications of this remarkable discovery. But before we do — why didn’t someone discover this earlier? The question is worth asking because the answer might indicate how modern science can still discover the unexpected. Clearly we were looking in the wrong direction. Most of our minds were fixed on two simple concepts:

  • The stomach secretes acid to keep it sterile.

  • Too much acid causes ulcers.

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References

  1. Black JW, Duncan WA, Durant CJ et al. Definition and antagonism of histamine H2-receptors. Nature 1972; 236: 385–390.

    Article  PubMed  CAS  Google Scholar 

  2. Elcenved G, Carlsson E, Cederberg C et al. Studies with H168/68, a novel gastric acid secretion inhibitor (abstract). Gut 1981; 22: A877.

    Google Scholar 

  3. Bottcher G. Dorpater Med Z 1874; 5:148.

    Google Scholar 

  4. Salomon H. Ueber das Spirillum des Saugetiermagens und sein Verhalten zu den Belegzellen. Zentralbl Bakteriol 1896; 19: 433–442.

    Google Scholar 

  5. Krienitz W. Ueber das Auftreten von Spirochaetne verschie-gener Form im Magen-inhalt bei Carcinoma ventriculi. Dtsch Med Wochenschr 1906; 32: 872.

    Article  Google Scholar 

  6. Palmer ED. Investigation of the gastric mucosa spirochaetes of the human. Gastroenterology 1954; 27:218–220.

    PubMed  CAS  Google Scholar 

  7. Luck JM, Seth TN. Gastric urease. Biochem J 1924; 18:1227–1231.

    PubMed  CAS  Google Scholar 

  8. Langenberg M, Tytgat GN, Schipper MEI et al. Campylobacter-like organisms in the stomach of patients and healthy individuals (letter). Lancet 1984; i: 1348–1349.

    Article  Google Scholar 

  9. Steer HW. Ultrastructure of cell migration through the gastric epithelium and its relationship to bacteria. J Clin Pathol 1975; 28: 639–646.

    Article  PubMed  CAS  Google Scholar 

  10. Fung WP, Papadimitriou JM, Matz LR. Endoscopic, histological and ultrastructural correlations in chronic gastritis. Am J Gastroenterol 1979; 71:269–279.

    PubMed  CAS  Google Scholar 

  11. Warren JR. Unidentified curved bacilli on gastric epithelium in active chronic gastritis (letter). Lancet 1983; i: 1273.

    Google Scholar 

  12. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984; i: 1311–1315.

    Article  Google Scholar 

  13. Rollason TP, Stone J, Rhodes JM. Spiral organisms in endoscopic biopsies of the human stomach. J Clin Pathol 1984; 37: 23–26.

    Article  PubMed  CAS  Google Scholar 

  14. Steer HW. Surface morphology of the gastroduodenal mucosa in duodenal ulceration. Gut 1984; 25:1203–1210.

    Article  PubMed  CAS  Google Scholar 

  15. Goodwin CS, Armstrong JA, Chilvers T et al. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. and Helicobacter pylori comb. nov. and Helicobacter mustelae comb, nov., respectively. Int J Syst Bacteriol 1989; 39: 397–405.

    Article  Google Scholar 

  16. Graham DY, Alpert LC, Smith JL, Yoshimura HH. Iatrogenic Campylobacter pylori infection is a cause of epidemic achlorhydria. Am J Gastroenterol 1988; 83:974–980.

    PubMed  CAS  Google Scholar 

  17. Cave DR, Vargas M. Effect of a Campylobacter pylori protein on acid secretion by parietal cells. Lancet 1989; ii: 187–189.

    Article  Google Scholar 

  18. Tache Y, Saperas E. Potent inhibition of gastric acid secretion and ulcer formation by centrally and peripherally administered interleukin-1. Ann N Y Acad Sci 1992; 664:353–368.

    Article  PubMed  CAS  Google Scholar 

  19. Berglund H, Chang HC. Transitory character of the achlorhydria during fever demonstrated by the histamine test. Proc R Soc Exp Biol Med 1929; 26:422–423.

    Article  CAS  Google Scholar 

  20. Hu LT, Mobley HL. Expression of catalytically active recombinant Helicobacter pylori urease at wild-type levels in Escherichia coli. Infect Immun 1993; 61:2563–2569.

    CAS  Google Scholar 

  21. Marshall BJ, Barrett LJ, Prakash C et al. Urea protects Helicobacter (Campylobacter) pylori from the bactericidal effect of acid. Gastroenterology 1990; 99:697–702.

    PubMed  CAS  Google Scholar 

  22. Eaton KA, Brooks CL, Morgan DR, Krakowka S. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect Immun 1991; 59:2470–2475.

    PubMed  CAS  Google Scholar 

  23. Butcher GP, Ryder SD, Hughes SJ et al. Use of an ammonia electrode for rapid quantification of Helicobacter pylori urease: its use in the endoscopy room and in the assessment of urease inhibition by bismuth subsalicylate. Digestion 1992; 53: 142–148.

    Article  PubMed  CAS  Google Scholar 

  24. Nagata K, Satoh H, Iwahi T et al. Potent inhibitory action of the gastric proton pump inhibitor lansoprazole against urease activity of Helicobacter pylori: unique action selective for H. pylori cells. Antimicrob Agents Chemother 1993; 37: 769–774.

    Article  PubMed  CAS  Google Scholar 

  25. Clayton CL, Kleanthous H, Morgan DD, Puckey L, Tabaqchali S. Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol 1993; 31:1420–1425.

    PubMed  CAS  Google Scholar 

  26. Labigne A, Cussac V, Courcoux P. Shuttle cloning and nucleotide sequences of Helicobacterpylori genes responsible for urease activity. J Bacteriol 1991; 173:1920–1931.

    PubMed  CAS  Google Scholar 

  27. Mauch F, Bode G, Malfertheiner P. Identification and characterization of an ATPase system of Helicobacter pylori and the effect of proton pump inhibitors (letter). Am J Gastroenterol 1993; 88:1801–1802.

    PubMed  CAS  Google Scholar 

  28. Logan RPH, Walker MM, Misiewicz JJ et al Changes in the intragastric distribution of Helicobacter pylori during treatment with omeprazole. Gut 1995; 36:12–16.

    Article  PubMed  CAS  Google Scholar 

  29. Unge P, Gad A, Gnarpe H, Olsson J. Does omeprazole improve antimicrobial therapy directed towards gastric Campylobacter pylori in patients with antral gastritis? A pilot study. Scand J Gastroenterol 1989; 24(suppl 167): 49–54.

    Article  Google Scholar 

  30. Eaton KA, Morgan DR, Krakowka S. Motility as a factor in the colonisation of gnotobiotic piglets by Helicobacter pylori. J Med Microbiol 1992; 37:123–127.

    Article  CAS  Google Scholar 

  31. Chen M, Lee A, Hazell S. Immunisation against gastric helicobacter infection in a mouse /Helicobacter felis model (letter). Lancet 1992; 339:1120–1121.

    Article  PubMed  CAS  Google Scholar 

  32. Doidge C, Crust I, Lee A et al Therapeutic immunisation against Helicobacter infection (letter). Lancet 1994; 343:914–915.

    Article  PubMed  CAS  Google Scholar 

  33. Nielsen H, Birkholz S, Andersen LP, Moran AP. Neutrophil activation by Helicobacter pylori lipopolysaccharides. J Infect Dis 1994; 170:135–139.

    Article  PubMed  CAS  Google Scholar 

  34. Hazell SL, Evans DJ Jr, Graham DY. Helicobacter pylori catalase. J Gen Microbiol 1991; 137:57–61.

    Article  PubMed  CAS  Google Scholar 

  35. Dunn BE, Roop RM, Sung CC et al. Identification and purification of a cpn60 heat shock protein homolog from Helicobacter pylori. Infect Immun 1992; 60:1946–1951.

    CAS  Google Scholar 

  36. Kaufmann SH, Schoel B, van Embden JD et al. Heat-shock protein 60: implications for pathogenesis of and protection against bacterial infections. Immunol Rev 1991; 121: 67–90.

    Article  PubMed  CAS  Google Scholar 

  37. Negrini R, Lisato L, Zanella I et al. Helicobacter pylori infection induces antibodies cross-reacting with human gastric mucosa. Gastroenterology 1991; 101:437–445.

    PubMed  CAS  Google Scholar 

  38. Narikawa S, Imai N, Sakama S et al [Evaluation of agar media for growth of Campylobacter pylori], Rinsho Byori 1990; 38: 104–106.

    PubMed  CAS  Google Scholar 

  39. Gold BD, Huesca M, Sherman PM, Lingwood CA. Helicobacter mustelae and Helicobacter pylori bind to common lipid receptors in vitro. Infect Immun 1993; 61:2632–2638.

    PubMed  CAS  Google Scholar 

  40. Lingwood CA, Wasfy G, Han H, Huesca M. Receptor affinity purification of a lipid-binding adhesin from Helicobacter pylori. Infect Immun 1993; 61:2474–2478.

    CAS  Google Scholar 

  41. Huang J, Keeling PW, Smyth CJ. Identification of erythrocyte-binding antigens in Helicobacter pylori. J Gen Microbiol 1992; 138: 1503–1513.

    Article  CAS  Google Scholar 

  42. Evans DG, Karjalainen TK, Evans DJ Jr et al Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori. J Bacteriol 1993; 175:674–683.

    CAS  Google Scholar 

  43. Boren T, Falk P, Roth KA et al Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens. Science 1993; 262:1892–1895.

    Article  PubMed  CAS  Google Scholar 

  44. Hessey SJ, Spencer J, Wyatt JI et al Bacterial adhesion and disease activity in Helicobacter associated chronic gastritis. Gut 1990;31:134–138.

    Article  PubMed  CAS  Google Scholar 

  45. Wyatt JI, Rathbone BJ, Sobala GM et al Gastric epithelium in the duodenum: its association with Helicobacter pylori and inflammation. J Clin Pathol 1990; 43:981–986.

    Article  PubMed  CAS  Google Scholar 

  46. Loffeld RJ, Ten Tije BJ, Arends JW. Prevalence and significance of Helicobacter pylori in patients with Barrett’s esophagus. Am J Gastroenterol 1992; 87:1598–1600.

    PubMed  CAS  Google Scholar 

  47. De Cothi GA, Newbold KM, O’Connor HJ. Campylobacter-like organisms and heterotopic gastric mucosa in Meckel’s diverticula. J Clin Pathol 1989; 42:132–134.

    Article  PubMed  Google Scholar 

  48. Dye KR, Marshall BJ, Frierson HF Jr et alCampylobacter pylori colonizing heterotopic gastric tissue in the rectum. Am J Clin Pathol 1990; 93:144–147.

    PubMed  CAS  Google Scholar 

  49. Nwokolo CU, Bickley J, Attard AR et al Evidence of clonal variants of Helicobacter pylori in three generations of a duodenal ulcer disease family. Gut 1992; 33:1323–1327.

    Article  PubMed  CAS  Google Scholar 

  50. Bamford KB, Bickley J, Collins JS et al. Helicobacter pylori: comparison of DNA fingerprints provides evidence for intraf amilial infection. Gut 1993; 34:1348–1350.

    Article  PubMed  CAS  Google Scholar 

  51. Cover TL, Blaser MJ. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J Biol Chem 1992; 267: 10570–10575.

    CAS  Google Scholar 

  52. Atherton JC, Cover TL, Peek RM, Blaser MJ. Subtyping of Helicobacter pylori strains into two groups by polymerase chain reaction amplification of the vac A gene and correlation of these groups with CagA status (abstract). Am J Gastroenterol 1994; 89: 1291.

    Google Scholar 

  53. Tummuru MK, Cover TL, Blaser MJ. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect Immun 1993; 61: 1799–1809.

    PubMed  CAS  Google Scholar 

  54. Crabtree JE, Taylor JD, Wyatt JI et al. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulceration, and gastric pathology. Lancet 1991; 338:332–335.

    Article  PubMed  CAS  Google Scholar 

  55. Luzzi I, Pezzella C, Caprioli A et al. Detection of vacuolating toxin of Helicobacter pylori in human faeces (letter). Lancet 1993; 341:1348.

    Article  PubMed  CAS  Google Scholar 

  56. Rautelin H, Blomberg B, Fredlund H et al. Incidence of Helicobacter pylori strains activating neutrophils in patients with peptic ulcer disease. Gut 1993; 34:599–603.

    Article  PubMed  CAS  Google Scholar 

  57. Kozol R, McCurdy B, Czanko R. A neutrophil chemotactic factor present in H. pylori but absent in H. mustelae. Dig Dis Sci 1993; 38:137–141.

    Article  CAS  Google Scholar 

  58. Craig PM, Territo MC, Karnes WE, Walsh JH. Helicobacter pylori secretes a chemotactic factor for monocytes and neutrophils. Gut 1992; 33:1020–1023.

    Article  PubMed  CAS  Google Scholar 

  59. Mooney C, Keenan J, Munster D et al. Neutrophil activation by Helicobacter pylori Gut 1991; 32:853–857.

    CAS  Google Scholar 

  60. Yoshida N, Granger DN, Evans DJ Jr et al. Mechanisms involved in Helicobacter pylori-induced inflammation. Gastroenterology 1993; 105:1431–1440.

    Article  PubMed  CAS  Google Scholar 

  61. Muotiala A, Heiander IM, Pyhala L et al. Low biological activity of Helicobacter pylori lipopolysaccharide. Infect Immun 1992; 60: 1714–1716.

    PubMed  CAS  Google Scholar 

  62. Langton SR, Cesareo SD. Helicobacter pylori associated phospho-lipase A2 activity: a factor in peptic ulcer production? J Clin Pathol 1992; 45:221–224.

    Article  PubMed  CAS  Google Scholar 

  63. Megraud F, Neman Simha V, Brugmann D. Further evidence of the toxic effect of ammonia produced by Helicobacter pylori urease on human epithelial cells. Infect Immun 1992; 60: 1858–1863.

    PubMed  CAS  Google Scholar 

  64. Cover TL, Vaughn SG, Cao P, Blaser MJ. Potentiation of Helicobacter pylori vacuolating toxin activity by nicotine and other weak bases. J Infect Dis 1992; 166:1073–1078.

    Article  PubMed  CAS  Google Scholar 

  65. Tsujii M, Kawano S, Tsuji S et al. Cell kinetics of mucosal atrophy in rat stomach induced by long-term administration of ammonia. Gastroenterology 1993; 104: 796–801.

    PubMed  CAS  Google Scholar 

  66. Tsujii M, Kawano S, Tsuji S et al. Ammonia: a possible promotor in Helicobacter pylori-related gastric carcinogenesis. Cancer Lett 1992;65:15–18.

    Article  PubMed  CAS  Google Scholar 

  67. Triebling AT, Korsten MA, Dlugosz JW et al. Severity of Helicobacter-induced gastric injury correlates with gastric juice ammonia. Dig Dis Sci 1991; 36:1089–1096.

    Article  PubMed  CAS  Google Scholar 

  68. El Nujumi AM, Rowe PA, Dahill S et al. Role of ammonia in the pathogenesis of the gastritis, hypergastrinaemia, and hyperpepsinogenaemia I caused by Helicobacter pylori infection. Gut 1992; 33:1612–1616.

    Article  PubMed  Google Scholar 

  69. Lee A. Animal models and vaccine development. In: Calam J, ed. Bailliere’s Clinical Gastroenterology: Helicobacter pylori. London: Baillière Tindall, 1995: (in press).

    Google Scholar 

  70. Fox JG, Edrise BM, Cabot EB et al. Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am J Vet Res 1986; 47: 236–239.

    PubMed  CAS  Google Scholar 

  71. Lee A, Hazell SL, O’Rourke J, Kouprach S. Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 1988; 56: 2843–2850.

    PubMed  CAS  Google Scholar 

  72. Wegmann W, Aschwanden M, Schaub N et al. [Gastritis associated with Gastrospirïllum hominis - a zoonosis?]. Schweiz Med Wochenschr 1991; 121:245–254.

    PubMed  CAS  Google Scholar 

  73. Lavelle J, Conklin F, Mitros S, Landas S. Transmission of ‘Gastrospirïllum hominis’ from cat to man. Gastroenterology 1992; 102: A651.

    Google Scholar 

  74. Dent JC, McNulty CA, Uff JC et al. Spiral organisms in the gastric antrum (letter). Lancet 1987; ii: 96.

    Article  Google Scholar 

  75. Lee A, Dent J, Hazell S, McNulty C. Origin of spiral organisms in human gastric antrum (letter). Lancet 1988; i: 300–301.

    Article  Google Scholar 

  76. Dick E, Lee A, Watson G, O’Rourke J. Use of the mouse for the isolation and investigation of stomach-associated, spiral-helical shaped bacteria from man and other animals. J Med Microbiol 1989;29:55–62.

    Article  PubMed  CAS  Google Scholar 

  77. Kuipers EJ, Pena AS, Van Kamp G et al. Seroconversion for Helicobacter pylori. Lancet 1993; 342:328–331.

    Article  PubMed  CAS  Google Scholar 

  78. Eaton KA, Radin MJ, Kramer L et al. Gastric spiral bacilli in captive cheetahs. Scand J Gastroenterol Suppl 1991; 181:38–42.

    Article  PubMed  CAS  Google Scholar 

  79. Fox JG, Lee A. Gastric Helicobacter infection in animals: natural and experimental infections. In: Goodwin CS, Worsley BW, eds. Helicobacter pylori: biology and clincal practice. Boca Raton, FL: CRC Press, 1993:407–430.

    Google Scholar 

  80. Bertram TA, Murray PD, Morgan DR et al Gastritis associated with infection by Helicobacter pylori in humans: geographical differences. Scand J Gastroenterol Suppl 1991; 181:1–8.

    Article  PubMed  CAS  Google Scholar 

  81. McColm AA, Bagshaw JA, O’Malley CF. Development of a 14C-urea breath test in ferrets colonised with Helicobacter mustelae: effects of treatment with bismuth, antibiotics, and urease inhibitors. Gut 1993; 34:181–186.

    Article  PubMed  CAS  Google Scholar 

  82. Henry GA, Long PH, Burns JL, Charbonneau DL. Gastric spiril-losis in beagles. Am J Vet Res 1987; 48:831–836.

    PubMed  CAS  Google Scholar 

  83. Handt LK, Fox JG, Dewhirst FE et al. Helicobacter pylori isolated from the domestic cat: public health implications. Infect Immun 1994; 62:2367–2374.

    PubMed  CAS  Google Scholar 

  84. Lee A, Chen M, Coltro N et al. Long term infection of the gastric mucosa with Helicobacter species does induce atrophic gastritis in an animal model of Helicobacter pylori infection. Int J Med Microbiol Virol Parasitol Infect Dis 1993; 280: 38–50.

    CAS  Google Scholar 

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© 1996 John Calam

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Calam, J. (1996). Discovery and bacteriology. In: Clinicians’ Guide to Helicobacter pylori . Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3350-8_1

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  • DOI: https://doi.org/10.1007/978-1-4899-3350-8_1

  • Publisher Name: Springer, Boston, MA

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