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Gene cloning in fungi and plants

  • Chapter
Gene Cloning

Part of the book series: Outline Studies in Biology ((OSB))

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Abstract

Yeast offers a number of advantages as a unicellular eukaryotic organism. It can be propagated in either the haploid or diploid state. It is capable of sporulation to form spore tetrads, from which individual haploid spores can be dissected and individually propagated. These natural advantages of the organism have led to its extensive genetic characterization and have played an important part in stimulating the development of gene cloning systems. DNA can be introduced into yeast cells which have been treated with a mixture of β-gluconases from snail gut extract to digest polysaccharide in the cell wall. The spheroplasts, so produced, can be maintained in isotonic solutions of sorbitol and DNA uptake can be promoted by the addition of polyethylene glycol. The cell wall regenerates if the treated spheroplasts are plated in 3% agar.

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Authors and Affiliations

  1. Cancer Research Campaign Eukaryotic Molecular Genetics Group, Department of Biochemistry, Imperial College of Science and Technology, London, UK

    David M. Glover

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  1. David M. Glover
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© 1984 D. M. Glover

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Glover, D.M. (1984). Gene cloning in fungi and plants. In: Gene Cloning. Outline Studies in Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3246-4_7

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  • DOI: https://doi.org/10.1007/978-1-4899-3246-4_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-412-26600-3

  • Online ISBN: 978-1-4899-3246-4

  • eBook Packages: Springer Book Archive

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