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Mutagenesis by ultraviolet and visible light II. Methods of mutation research on micro-organisms and mammalian cell cultures

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Mutation research

Abstract

The most important experiments on the mutagenic effects of ultraviolet and visible light have been carried out on micro-organisms. For a critical appreciation of the results, it is necessary to understand the rationale, scope and limitations of the test methods, and the kind of conclusions that can or cannot be drawn validly from the data. I have dealt with this aspect of the methodology more fully in a previous book, in which additional references to the literature up to 1961 can also be found (‘Mutation’; Bibliography). Detailed descriptions of techniques are found in ‘Chemical Mutagens’ Bibliography).

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References

  1. Hollaender, A. and Emmons, C.W. (1941), ‘Wavelength dependence of mutation production in the ultraviolet with special emphasis on fungi’, Cold Spring Harbor Symp. Quant. Biol., 9, 179–186.

    Article  Google Scholar 

  2. Beadle, G.W. and Tatum, E.L. (1945), ‘Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements’, Am. J. Bot. 32, 678.

    Article  Google Scholar 

  3. Demerec, M. (1951), ‘Studies of the streptomycin-resistance system of mutations in E. coli’, Genetics, 36, 585–597.

    PubMed  CAS  Google Scholar 

  4. Auerbach, C. (1970), ’Remark on the ‘Tables for determining the statistical significance of mutation frequencies’, Mutation Res., 10, 256.

    Article  PubMed  CAS  Google Scholar 

  5. Bürk, R.R., Pitts, J.D. and Subak-Sharpe, J.H. (1968), ‘Exchange between hamster cells in culture’, Exp. Cell Res., 53, 297–301.

    Article  PubMed  Google Scholar 

  6. Auerbach, C. and Ramsay, D. (1971), ‘The problem of viability estimates in tests for reverse mutations’, Mutation Res., 11, 353–360.

    PubMed  CAS  Google Scholar 

  7. Witkin, E.M. (1967), ‘Mutation-proof and mutation-prone modes of survival in derivatives of Escherichia coli B differing in sensitivity to ultraviolet light’, Brookhaven Symposia in Biology, 20, 17–55.

    Google Scholar 

  8. Atwood, K.C., Mukai, F. and Pittenger, T.H. (1958), ‘“Punch tube” and “squirting” methods for Neurospora’, Microbial Genetics Bull, 16, 34–35.

    Google Scholar 

  9. De Serres, F.J. (1968), Genetic analysis of the extent and type of functional inactivation in irreparable recessive lethal mutations in the ad-3 region of Neurospora crassa, Genetics, 58, 69–77.

    PubMed  Google Scholar 

  10. Hong, J.S. and Ames, B.N. (1971), ‘Localized mutagenesis of any specific small region of the bacterial chromosome’, Proc. Nat. Acad. Sci. U.S.A. 68, 3158–3162.

    Article  CAS  Google Scholar 

  11. Kinsey, J.A. and Stadler, D.R. (1969), ‘Interaction between analogue resistance and amino acid auxotrophy in Neurospora’, J. Bacter., 97, 1114–1117.

    CAS  Google Scholar 

  12. Alderson, T. and Scazzocchio, C. (1967), ‘A system for the study of interlocus specificity for both forward and reverse mutation in at least eight gene loci in Aspergillus nidulans’, Mutation Res. 4, 567–577.

    Article  PubMed  CAS  Google Scholar 

  13. De Serres, F.J. and Rømark, H.G. (1958), A direct method for determination of forward mutation rates in Neurospora crassa, Nature, 182, 1249–1250.

    Article  Google Scholar 

  14. Roman, H. (1956), ‘A system selective for mutations affecting the synthesis of adenine in yeast’, C.R. Labor. Carlsberg, 26, 299–314.

    CAS  Google Scholar 

  15. Leupold, L.S. (1955), ‘Versuche zur Genetischen Klassifizierung Adeninn-Abhängiger Mutanten von Schizosaccharomyces pombe’, Arch. Julius Klaus-Stift. Vererbungsforsch. Sozialanthropol. Rassenhyg., 30, 506–516.

    Google Scholar 

  16. Lederberg, J. (1950), ‘Isolation and characterisation of biochemical mutants of bacteria’, Methods in Medical Research, 3, 5–22.

    Google Scholar 

  17. Luria, S.E. and Delbrück, M. (1943), ‘Mutations of bacteria from virus sensitivity to virus resistance’, Genetics, 28, 491–511.

    PubMed  CAS  Google Scholar 

  18. Novick, A. and Szilard, L. (1950), ‘Description of the Chemostat’, Science, 112, 715–716.

    Article  PubMed  CAS  Google Scholar 

  19. Munson, R.J. and Jeffrey, A. (1964), ‘Reversion rate in continuous cultures of an Escherichia coli auxotroph exposed to γ rays’, J. Gen. Microbiol., 35, 191–203.

    Article  PubMed  CAS  Google Scholar 

  20. Bryson, V. (1952), ‘Microbial selection. Part II: The Turbidostatic Selector — a device for automatic isolation of bacterial variants’, Science, 116, 48–51.

    Article  Google Scholar 

  21. Newcombe, H.B. (1949), Origin of bacterial variants’, Nature, 164, 150.

    Article  PubMed  CAS  Google Scholar 

  22. Lederberg, J. and Lederberg, E.M. (1952), ‘Replica plating and indirect selection of bacterial mutants’, J. Bacter., 63, 399.

    CAS  Google Scholar 

  23. Vogt, M., Dulbecco, R. and Wenner, H.A. (1957), ‘Mutants of poliomyelitis viruses with reduced efficiency of plating in acid medium and reduced neuropathogenicity’, Virology, 4, 141–155.

    Article  PubMed  CAS  Google Scholar 

  24. Mundry, K.W. and Gierer, A. (1958), ‘Die Erzeugung von Mutationen des Tabakmosaikvirus durch chemische Behandlung seiner Nukleinsäure in vitro’, Zeitsch. Vererb. Lehre, 89, 614–630.

    CAS  Google Scholar 

  25. Litman, R. and Ephrussi-Taylor, H. (1959), ‘Inactivation et mutation des facteurs génétiques de l’acide desoxyribonucléique du pneumocoque par l’ultraviolet et par l’acide nitreux’, C.R. Acad. Sc., 249, 838–840.

    CAS  Google Scholar 

  26. Freese, E. and Strack, H.B. (1962), ‘Induction of mutation in transforming DNA by hydroxylamine’, Proc. Nat. Acad. Sci. U.S.A., 48, 1796–1803

    Article  CAS  Google Scholar 

  27. Baltz, R.H. and Drake, J.W. (1972), ‘Bacteriophage T4 transformation: an assay for mutations induced in vitro’, Virology, 49, 462–474.

    Article  PubMed  CAS  Google Scholar 

  28. Kao, F.T. and Puck, T.T. (1968), ‘Genetics of somatic mammalian cells. VII Induction and isolation of nutritional mutants in Chinese hamster cells’, Proc. Nat. Acad. Sci., U.S.A. 60, 1275–1281.

    Article  CAS  Google Scholar 

  29. Szybalski, W., Ragni, G. and Cohn, N.J. (1964), ‘Mutagenic response of human somatic cell lines’, Symp. Int. Soc. Cell Biol., 3, 209–221.

    Google Scholar 

  30. Albertini, R.J. and de Mars, R. (1970), ‘Diploid azaguanineresistant mutants of cultured human flbroblasts’, Science, 169, 482–485.

    Article  CAS  Google Scholar 

  31. Clive, D., Flamm, W.G., Macheska, M.R. and Bernheim, N.J. (1972), ‘Amutational assay system using the thymidine kinase locus in mouse lymphoma cells’, Mutation Res., 16, 77–87.

    Article  PubMed  CAS  Google Scholar 

  32. Harris, M. (1971), ‘Mutation rates in cells at different ploidy levels’, J. Cell. Physiol., 78, 177–184.

    Article  PubMed  CAS  Google Scholar 

  33. Sharp, J.D., Capecchi, N.E. and Capecchi, M.R. (1973), ‘Altered enzymes in drug-resistant variants of mammalian tissue culture cells’, Proc. Nat. Acad. Sci. U.S.A. 70, 3145–3149.

    Article  CAS  Google Scholar 

  34. Beaudet, A.L., Roufa, D.J. and Caskey, C.T. (1973), ‘Mutation affecting the structure of hypoxanthine: guanine phosphoribosyltransferase in cultured Chinese hamster cells’, Proc. Nat. Acad. Sct., U.S.A., 70, 320–324.

    Article  CAS  Google Scholar 

  35. Orgel, A. and Brenner, S. (1961), ‘Mutagenesis of bacteriophage T4 by acridines’, J. Mol. Biol., 3, 762–768.

    Article  PubMed  CAS  Google Scholar 

  36. Streisinger, G., Okada, Y., Emrich, J., Newton, J., Tsugita, A., Terzaghi, E. and Inouye, M. (1966), ‘Frameshift mutations and the Genetic Code’, Cold Spring Harbor Symp. Quant. Biol., 31, 77–84.

    Article  PubMed  CAS  Google Scholar 

  37. Yanofsky, C., Ito, J. and Horn, V. (1966), ‘Amino acid replacements and the Genetic Code’, Cold Spring Harbor Symp. Quant Biol., 31, 151–162.

    Article  PubMed  CAS  Google Scholar 

  38. Prakash, L. and Sherman, F. (1973), ‘Mutagenic specificity: reversion of iso-1-cytochrome-c mutants of yeast’, J. Mol. Biol., 79, 65–82.

    Article  PubMed  CAS  Google Scholar 

  39. Person, S. and Osborn, M. (1968), ‘The conversion of amber suppressors to ochre suppressors’, Proc. Nat. Acad. Sct., U.S.A., 60, 1030–1037.

    Article  CAS  Google Scholar 

  40. Ripley, L.S. and Drake, J.W. (1972), ‘A genetic assay for transversion mutations in bacteriophage T4’, Mol. Gen. Genet., 118, 1–10.

    Article  PubMed  CAS  Google Scholar 

  41. Osborn, M., Person, S., Phillips, S. and Funk, F. (1967), ‘A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4’, J. Mol. Biol., 26, 437–447.

    Article  PubMed  CAS  Google Scholar 

  42. De Serres, F.J. (1964), ‘Mutagenesis and chromosome structure’, J. Cell. Comp. Physiol., 64, Suppl. 1, 33–42.

    Article  Google Scholar 

  43. De Serres, F.J. and Mailing, H.V. (1969), ‘Identification of the genetic alterations in specific locus mutants at the molecular level’, Jap. J. Genetics, 44, Suppl. 1., 106–113.

    Google Scholar 

  44. De Serres, F.J., Brockman, H.E., Barnett, W.E. and Kølmark, H.G. (1967), ‘Allelic complementation among nitrous acidinduced ad-3B mutants of Neurospora crassa’, Mutation Res., 4, 415–424.

    Article  PubMed  Google Scholar 

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© 1976 Charlotte Auerbach

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Auerbach, C. (1976). Mutagenesis by ultraviolet and visible light II. Methods of mutation research on micro-organisms and mammalian cell cultures. In: Mutation research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3103-0_11

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  • DOI: https://doi.org/10.1007/978-1-4899-3103-0_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-412-11280-5

  • Online ISBN: 978-1-4899-3103-0

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