Abstract
Over the past decade, the techniques used for the isolation of flavonoids from plant material have not changed drastically Review articles on this general topic by Seshadri (1962) and Seikel (1962) emphasized the importance of solvent extraction, crystalliz ation, and column and paper chromatography, and these techniques are still very much in use today. Thin-layer chromatography, sephadex gel chromatography and gas-liquid chromatography are perhaps the three major innovations since that time. The increasing use of these essentially small scale methods (together with paper chromatography) has been brought about largely by two factors: (1) the need to isolate only small amounts of material for structure analysis and (1i) the availability of only limited quantities of plant materials for phyto chemical surveys.
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Markham, K.R. (1975). Isolation Techniques for Flavonoids. In: Harborne, J.B., Mabry, T.J., Mabry, H. (eds) The Flavonoids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2909-9_1
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