Abstract
The aim of this chapter is to introduce the procedures currently used to isolate and manipulate yeast genes. We restrict our discussion to those procedures that are, at least in part, peculiar to the yeast Saccharomyces cerevisiae. We do not deal with procedures that are common to all other organisms, such as cDNA bank construction, hybrid-selected or arrested translation systems, nucleic acid probe strategies, or radioimmune detection strategies, as these have been covered in a variety of general texts (e.g., Maniatis et al., 1982; Glover, 1985).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beggs, J. D. 1978, Transformation of yeast by a replicating hybrid plasmid, Nature, 275: 104–109.
Boeke, J. D., Lacroute, F., and Fink, G. R. 1984, A positive selection for mutants lacking orotidine-5’-phosphate decarboxylase activity in yeast: 5-Fluoro-orotic acid resistance, Mol. Gen. Genet. 197: 345–346.
Broach, J. R. 1982, The yeast plasmid 2 µ circle, Cell 28: 203–204.
Burke, D. T., and Olson, M. V. 1986, Oligo-directed mutagenesis of E. coli and yeast by simple co-transformation of the primer and template, DNA 5: 325–332.
Burke, D. T., Carle, G. F., and Olsen, M. V. 1987, Cloning of large segments of exogenous DNA into yeast by means of artificial yeast chromosome vectors, Science 236: 806–813.
Butt, T. R., Sternberg, E. J., Gorman, J. A., Clark, P., Hamer, D., Rosenberg, M., and Crooke, S. T. 1984, Copper metallothionein of yeast: Structure of the gene and regulation of expression, Proc. Natl. Acad. Sci. USA 81: 3332–3336.
Cesareni, G., and Murray, J. A. H. 1987, Plasmid vectors carrying the replication origin of filamentous single-stranded phages, In: I. K. Setlow, ed., Genetic Engineering, Vol. 9, Plenum Press, New York, pp. 137–144.
Clarke, L., and Carbon, J. 1976, A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome, Cell 9: 91–99.
Clarke, L., and Carbon, J. 1980, Isolation of a yeast centromere and construction of functional small circular chromosomes, Nature 257: 504–509.
Glover, D. M. 1985, DNA Cloning. Vol. 1/II. A Practical Approach, IRL Press, Oxford.
Henderson, R. C. A., Cox, B. S., and Tubb, R. 1985, The transformation of brewing yeasts with a plasmid containing the gene for copper resistance, Curr. Genet. 9: 133–138.
Hinnen, A., Hicks, J. B., and Fink, G. R. 1978, Transformation of yeast, Proc. Natl. Acad. Sci. USA 75: 1929–1933.
Ito, H., Fukuda, Y., Murata, K., and Kimura, A. 1983, Transformation of intact yeast cells treated with alkali cations, J. Bacteriol. 153: 163–168.
Jayaram, M., Li, Y.-Y., and Broach, J. R. 1983, The yeast plasmid 2 µ circle encodes components required for its high copy number propagation, Cell 34: 95–104.
Jiminez, A., and Davies, J. 1980, Expression of a transposable antibiotic resistance element in Saccharomyces, Nature 287: 869–871.
Kingsman, A. J., Clarke, L., Mortimer, R. K., and Carbon, J. 1979, Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast TRP1 region, Gene 7: 141–152.
Maniatis, T., Fritsch, E. F., and Sambrook, J. 1982, Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Moerschell, R. P., Tsunasawa, S., and Sherman, F. 1988, Transformation of yeast with synthetic oligonucleotides, Proc. Natl. Acad. Sci. USA 85: 524–528.
Mortimer, R. K., and Schild, D. 1985, Genetic map of Saccharomyces cerevisiae, edition 9, Microbiol. Rev. 49: 181–213.
Murray, A. W, and Szostak, J. W. 1983a, Pedigree analysis of plasmid segregation in yeast, Cell 34: 961–970.
Murray, A. W., and Szostak, J. W. 1983b, Construction of artificial chromosomes in yeast, Nature 305: 189–193.
Nasmyth, K. 1985, At least 1400 base pairs of 5’-flanking DNA is required for the correct expression of the HO gene in yeast, Cell 42: 213–223.
Nasmyth, K., and Reed, S. I. 1980, Isolation of genes by complementation in yeast: Molecular cloning of a cell-cycle gene, Proc. Natl. Acad. Sci. USA 77: 2119–2123.
Nasmyth, K., and Tatchell, K. 1980, The structure of transposable yeast mating-type loci, Cell 19: 753–764.
Orr-Weaver, T. L., and Szostak, W. J. 1983, Yeast recombination: The association between double-strand gap repair and crossing-over, Proc. Natl. Acad. Sci. USA 80: 4417–4421.
Orr-Weaver, T. L., Szostak, J. W., and Rothstein, R. J. 1981, Yeast transformation: A model system for the study of recombination, Proc. Natl. Acad. Sci. USA 78: 6354–6358.
Orr-Weaver, T. L., Szostak, J. W., and Rothstein, R. J. 1982, Genetic applications of yeast transformations with linear and gapped plasmids. In: R. Wu, L. Grossman, and K. Moldave, eds., Methods in Enzymology Vol. 101, Academic Press, New York, pp. 228–245.
Parent, S. A., Fenimore, C. M., and Bostian, K. A. 1985, Vector systems for the expression, analysis and cloning of DNA sequences, S. cerevisiae, Yeast 1: 83–138.
Ratzkin, B., and Carbon, J. 1977, Functional expression of cloned yeast DNA, E. coli, Proc. Natl. Acad. Sci. USA 74: 487–491.
Rothstein, R. J. 1983, One-step gene disruption in yeast, In: R. Wu, L. Grossman, and K. Moldave, eds., Methods in Enzymology, Vol. 101, Academic Press, New York, pp. 202–211.
Rothstein, R. J. 1985, Cloning in yeast, In: D. M. Glover, ed., DNA Cloning. Vol. II. A Practical Approach,IRL Press, Oxford, pp. 45–66.
Rudolph, H., Koenig-Rauseo, I., and Hinnen, A. 1985, One-step gene replacement in yeast by cotransformation, Gene 36: 87–95.
Scherer, S., and Davis, R. W. 1979, Replacement of chromosome segments with altered DNA sequences constructed in vitro, Proc. Natl. Acad. Sci. USA 76: 4951–4955.
Shortie, D., Haber, J. E., and Botstein, D. 1982, Lethal disruption of the yeast actin gene by integrative DNA transformation, Science 217: 371–373.
Singh, H., Bieker, J. J., and Dumas, L. B. 1982, Genetic transformation of Saccharomyces cerevisiae with single-stranded circular DNA vectors, Gene 20: 441–449.
Southern, E. M. 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98: 503–517.
Stiles, J. I., Szostak, J. W., Young, A. T., Wu, R., Consaul, S., and Sherman, F. 1981, DNA sequence of a mutation in the leader region of the yeast iso-l-cytochrome mRNA, Cell 25: 277–284.
Stinchcomb, D. T., Struhl, K., and Davis, R. W. 1979, Isolation and characterisation of a yeast chromosomal replicator, Nature 282: 39–43.
Struhl, K. 1983, The new yeast genetics, Nature 305: 391–396.
Struhl, K„ Cameron, J. R., and Davis, R. W. 1976, Functional genetic expression of eukaryotic DNA in E. coli, Proc. Natl. Acad. Sci. USA 73: 1471–1475.
Struhl, K., Stinchcomb, D. T., Scherer, S., and Davis, R. W. 1979, High frequency transformation of yeast; autonomous replication of hybrid molecules, Proc. Natl. Acad. Sci. USA 76: 1035–1039.
Walder, R. Y., and Walder, J. A. 1986, Oligodeoxynucleotide-directed mutagenesis using the yeast transformation system, Gene 42: 133–139.
Williamson, D. H. 1985, The yeast ARS element, six years on: A progress report, Yeast 1: 114.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kingsman, A.J., Mellor, E.J., Dobson, M.J., Kingsman, S.M. (1991). Recombinant DNA Techniques. In: Tuite, M.F., Oliver, S.G. (eds) Saccharomyces. Biotechnology Handbooks, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2641-8_5
Download citation
DOI: https://doi.org/10.1007/978-1-4899-2641-8_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-2643-2
Online ISBN: 978-1-4899-2641-8
eBook Packages: Springer Book Archive