Abstract
Since in higher plants—at least in a number of species—it is possible to regenerate the whole organism from single somatic cells, here cell fusion techniques can be regarded not only as a means of analysis at the cellular level, but also as a means of creating new organisms. Protoplast fusion for the production of somatic hybrids (for reviews see: Evans, 1983; Evans et al., 1983; Gleba and Sytnik, 1984; Harms, 1985) or for the transfer of cytoplasmic traits (Galun and Aviv, 1983) is generally performed in a statistical manner, i. e., fusion is induced in a mixed population of the respective fusion partners. This approach allows only limited, if any, control of the type and numbers of cells participating in a particular fusion event and therefore requires identification and selection of putative fusion products either shortly after the fusion procedure (Kao, 1977) or during later stages of culture (Melchers and Labib, 1974). The availability of selectable markers may be a limiting factor for the applicability of cell fusion techniques in genotypes qualifying for use in breeding programs. Moreover, in the case of subprotoplast fusion for transfer of cytoplasmic traits, i. e., in the case of cell reconstitution experiments, unavoidable contamination of subprotoplast preparations with different subprotoplasts or intact protoplasts (Wallin et al., 1978, 1979; Maliga et al., 1982) may considerably reduce the value of this technique by obscuring the identity and composition of particular fusion products, cell lines, or plants regenerated thereof.
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Koop, HU., Spangenberg, G. (1989). Electric Field-Induced Fusion and Cell Reconstitution with Preselected Single Protoplasts and Subprotoplasts of Higher Plants. In: Neumann, E., Sowers, A.E., Jordan, C.A. (eds) Electroporation and Electrofusion in Cell Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2528-2_23
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DOI: https://doi.org/10.1007/978-1-4899-2528-2_23
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