Abstract
Electrofusion is a technique which uses the application of precise, transient electric fields to induce somatic cell hybridization (Zimmermann, 1982; Knight and Scrutton, 1986). This recent methodology is effective for obtaining viable hybrids of plant (Bates et al., 1983; Saunders, 1985), yeast (Schnettler et al., 1984), and mammalian (Scheurich and Zimmermann, 1981; Tiessié et al., 1982; Vienken et al., 1983; Finaz et al., 1984; Lo et al., 1984; Neumann, 1984; Podesta et al., 1984; Karsten et al., 1985; Pratt et al., 1987) origin, including interkingdom hybridization (Cocking, 1984). Hybridomas of both mouse (Berg et al., 1983; Shimizu and Watarobe, 1985; Wojchowski and Sytkowski, 1986) and human (Bischoff et al., 1982; Shimizu and Watarobe, 1985; Pratt et al., 1987) derivation have been produced at relatively high efficiency, but Ig secretion has been limited (Vienken and Zimmermann, 1985; Hofmann and Evans, 1986).
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Glassy, M.C., Pratt, M. (1989). Generation of Human Hybridomas by Electrofusion. In: Neumann, E., Sowers, A.E., Jordan, C.A. (eds) Electroporation and Electrofusion in Cell Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2528-2_17
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DOI: https://doi.org/10.1007/978-1-4899-2528-2_17
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