Abstract
The giant axon of the squid has been the preparation of choice for the recording of electrical events associated with the opening and closing of the ionic conductances. Its large size allows the introduction of electrodes and the exchange of solutions giving almost complete control of the chemical environment and voltage across the axolemma (Adelman and Gilbert, 1990; Mullins and Brinley, 1990).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adelman, W. J., Jr. and Gilbert, D. L., 1990, Electrophysiology and biophysics of the squid axon, this volume.
Bekkers, J. M., Greeff, N. G., and Keynes. R. D., 1986, The conductance and density of sodium channels in the cut-open squid giant axon, J. Physiol. 377: 463–486.
Bezanilla, F., 1987, Single sodium channels from the squid giant axon, Biophys. J. 52: 1087–1090.
Bezanilla, F. and Armstrong, C. M., 1977, Inactivation of the sodium channel. I. Sodium current experiments, J. Gen. Physiol. 70: 549–566.
Conti, F. and Neher, E., 1980, Single channel recording of K currents in squid axons, Nature 285: 140–143.
Correa, A. M., Latorre, R., and Bezanilla. F., 1989, Na-dependence and temperature effects on BTX-treated sodium channels in the squid giant axon, Biophys. J. 55: 403a (Abstr.).
Forster, I. C. and Greeff, N. G., 1988, Technical aspects of voltage-clamping the cut-open squid giant axon, J. Neurosci. Methods 26: 151–168.
Levis, R. A., Bezanilla, F., and Tones, R. M., 1984, Estimate of the squid axon sodium channel conductance with improved frequency response, Biophys. J. 45:1la (Abstr.).
Llano, I. and Bezanilla F., 1980, Current recorded from a cut-open giant axon under voltage clamp, Proc. Nat. Acad. Sci. 77: 7484–7486.
Llano, I. and Bezanilla F., 1983, Bursting activity of potassium channels in the cut-open axon, Biophys. J. 41: 38a. (Abstr.)
Llano, I. and Bezanilla F., 1984, Analysis of sodium current fluctuations in the cut-open squid giant axon, J. Gen. Physiol. 83: 133–142.
Llano, I. and Bezanilla F., 1985, Two types of potassium channels in the cut-open squid giant axon, Biophys. J. 47: 221a. (Abstr.).
Llano, I., Webb, C. K., and Bezanilla, F., 1988, Potassium conductance of the squid giant axon. Single-channel studies, J. Gen. Physiol. 92: 179–196.
Lopez-Bameo, J., Matteson, D. R., and Armstrong C. M., 1981, Currents recorded through small areas of squid axon membrane with an internal virtual ground voltage clamp, Biophys. J. 36: 811–816.
Mullins, L. J. and Brinley, F. J., Jr., 1990, Internal dialysis in the squid axon, this volume. Perozo, E., Bezanilla, F., and Dipolo R., 1989, Modulation of K channels in dialyzed squid axons: ATP mediated phosphorylation, J. Gen. Physiol. 93: 1195–1218.
Schauf, C. L., 1987, Properties of single Na channels in cut-open Myxicola giant axons, Can. J. Physiol. Pharm. 65: 568–573.
Sigworth, F. J., 1980, The variance of sodium current fluctuations at the node of Ranvier, J. Physiol. 307: 97–129.
Vandenberg, C. A. and Bezanilla, F., 1988, Single-channel, macroscopic and gating currents from Na channels in squid giant axon, Biophys. J. 53: 226a (Abstr.).
Vandenberg, C. A. and Bezanilla, F., 1989, in prep.
Vandenberg, C., Perozo, E., and Bezanilla, F., 1989, ATP modulation of the K current in squid giant axon: a single channel study, Biophys. J. 55: 586a (Abstr.).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Bezanilla, F., Vandenberg, C. (1990). The Cut-Open Axon Technique. In: Gilbert, D.L., Adelman, W.J., Arnold, J.M. (eds) Squid as Experimental Animals. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2489-6_9
Download citation
DOI: https://doi.org/10.1007/978-1-4899-2489-6_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-2491-9
Online ISBN: 978-1-4899-2489-6
eBook Packages: Springer Book Archive