Abstract
In the context of this paper we shall discuss methods of measuring the diffusion of small molecules through the extracellular space of the brain. The study of such diffusion properties has relevance to the transport of metabolites, the spread of neuromodulators and the application of drugs to the brain. The extracellular space (ECS) is a component of the brain cell microenvironment (Schmitt and Samson 1969, Nicholson 1980, Cserr 1986). The movement of neuromodulators and neuroactive substances through this environment - so-called volume transmission - has been the topic of a recent symposium (Fuxe and Agnati 1991).
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References
Aikens, R. S., Agard, D. A. & Sedat, J. W. (1989). Solid-state imagers for microscopy. In: Methods in Cell Biology. Volume 29. “Fluorescent Microscopy of Living Cells in Culture. Part A. Fluorescent Analogs, Labeling Cells, and Basic Microscopy”. eds. Wang, Y.-L. and Taylor, D. L. San Diego: Academic, pp 291–313.
Ammann, D. (1986). “Ion-Selective Microelectrodes”. Berlin: Springer-Verlag.
Blasdel, G. G. (1989). Visualization of neuronal activity in monkey striate cortex. Ann. Rev. Physiol. 51, 561–581.
Cserr, H. F. (1986) The neuronal microenvironment. Ann. N. Y. Acad, Sci., 481: 1–391.
Cserr, H. F., DePasquale, M., Nicholson, C, Patlak, C. S., Pettigrew, K. D. and Rice, M. E. (1991) Extracellular volume decreases while cell volume is maintained by uptake of ions in rat cerebral cortex during acute hypernatremia. J. Physiol. 442: 277–295.
Chesler, M. (1990) The regulation and modulation of pH in the nervous system. Prog Neurobiology 34: 401–427.
Cohen, L., Hopp, H-P., Wu, J-Y. and Xiao, C. (1989) Optical measurements of action potential activity in invertebrate ganglia. Annual. Rev. Physiol. 51: 527–541.
Fenstermacher, J. D. and Kaye, T. (1988) Drug “diffusion” within the brain. Ann. N. Y. Acad. Sci. 531: 29–39.
Fuxe, K. and Aganati, L. F. (1991) “Volume Transmission in the Brain: Novel Mechanisms for Neuronal Transmission”. Raven, New York.
Lieke, E. E., Frostig, R. D., Arieli, A., Ts’o, D. Y., Hildesheim, R. and Grinvald, A. (1989) Optical imaging of cortical activity. Ann. Rev. Physiol. 51: 543–559.
Lux, H. D. and Neher, E. (1973) The equilibration time course of [K+]o in cat cortex. Exp Brain Res 17: 190–205.
McBain, C. J., Traynelis, S. F. and Dingledine, R. (1990) Regional variation of extracellular space in the hippocampus. Science: 249: 674–677.
Nicholson, C. (1980) Dynamics of the brain cell microenvironment. Neurosci. Res. Prog. Bull. 18: 177–322.
Nicholson, C. (1985) Diffusion from an arbitrary volume of a substance in brain tissue with arbitrary volume fraction and tortuosity. Brain Res. 333: 325–329.
Nicholson, C. (1992). Quantitative analysis of extracellular space using the method of TMA+ iontophoresis and the issue of TMA+ uptake. Can. J. Physiol. Pharmacol. (in press).
Nicholson, C. and Phillips, J. M. (1981) Ion diffusion modified by tortuosity and volume fraction in the extracellular microenvironment of the rat cerebellum. J. Physiol. 321: 225–257.
Nicholson, C, Phillips, J. M. and Gardner-Medwin A. R. (1979) Diffusion from an iontophoretic point source in the brain: role of tortuosity and volume fraction. Brain Res 169: 580–584.
Nicholson, C. and Rice, M. E. (1986a) The migration of substances in the neuronal microenvironment. Ann. N. Y. Acad. Sci. 481: 55–71.
Nicholson, C. and Rice, M. E. (1986b) Diffusion characteristics of skate (Rajaerinacea) cerebellum measured with tetramethylammonium and ion-selective microelectrodes. Mt. Desert Island Biol. Bull. 25: 54–55.
Nicholson, C. and Rice, M. E. (1988). Use of ion-selective microelectrodes and voltammetric microsensors to study brain cell microenvironment. In: “Neuromethods; The Neuronal Microenvironment”. Eds. Boulton, A. A., Baker, G. B. & Walz, W. Clifton, NJ: Humana, pp 247–361.
Nicholson, C. and Rice, M. E. (1991) Diffusion of ions and transmitters in the brain cell microenvironment. In: “Volume Transmission in the Brain”. Eds. K. Fuxe and L. F. Agnati. New York: Raven, pages 279–294.
Nugent. L. J. and Jain, R. K. (1984a). Plasma pharmacokinetics and interstitial diffusion of macromolecules in a cappillary bed. Am. J. Physiol. 246: H129–H137.
Nugent. L. J. and Jain, R. K. (1984b) Extravascular diffusion in normal and neoplastic tissues. Cancer Res. 44: 238–244.
Oehme, M. and Simon, W. (1976) Microelectrode for K+ based on a neutral carrier and comparison of its characteristics with a cation exchanger sensor. Analyticaxhim. Acta. 86: 21–25.
Press, W. H., Flannery, B. P., Teukolsky S. A., and Vetterling, W. T. (1986) “Numerical Recipes”. Cambridge University Press, London. Chapter 10.
Rice, M. E. and Nicholson, C. (1989) Measurement of nanomolar dopamine diffusion using low-noise perflorinated ionomer coated carbon fiber microelectrodes and high-speed cyclic voltammetry. Analyt. Chem. 61: 1805–1810.
Rice, M. E. and Nicholson, C. (1991) Diffusion characteristics and extracellular volume fraction during normoxia and hypoxia in slices of rat neostriatum. J. Neurophysiol. 65: 264–272.
Safranyos, R. G. A. and Caveney, S. (1985) Rates of diffusion of fluorescent molecules via cell-to-cell membrane channels in a developing tissue. J. Cell Biol. 100: 736–747.
Schmitt, F. O. (1984) Molecular regulators of brain function: A new view. Neuroscience 13: 991–1001.
Schmitt, F. O. and Samson, F. E. Jr. (1969) Brain cell microenvironment. Neurosci. Res. Progr. Bull. 7: 277–417.
Spring, K. R. and Lowy, R. J. (1989) Characteristics of low light level television cameras. In: Methods in Cell Biology. Volume 29. “Fluorescent Microscopy of Living Cells in Culture. Part A. Fluorescent Analogs, Labeling Cells, and Basic Microscopy”, eds. Wang, Y.-L. and Taylor, D. L. San Diego: Academic, pp 269–289.
Taylor, D. L. and Wang, Y.-L. eds. (1989). Methods in Cell Biology. Volume 30. “Fluorescent Microscopy of Living Cells in Culture. Part B. Quantitative Fluorescence Microscopy-Imaging and Spectroscopy”. San Diego: Academic.
Tsien, R. Y. (1989). Fluorescent indicators of ion concentration. In: Methods in Cell Biology. Volume 29. “Fluorescent Microscopy of Living Cells in Culture. Part A. Fluorescent Analogs, Labeling Cells, and Basic Microscopy”, eds. Wang, Y.-L. and Taylor, D. L. San Diego: Academic, pp 127–156.
Wang, Y.-L. and Taylor, D. L. eds. (1989). Methods in Cell Biology. Volume 29. “Fluorescent Microscopy of Living Cells in Culture. Part A. Fluorescent Analogs, Labeling Cells, and Basic Microscopy”. San Diego: Academic.
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Nicholson, C., Tao, L. (1993). Diffusion Properties of Brain Tissue Measured with Electrode Methods and Prospects for Optical Analysis. In: Dirnagl, U., Villringer, A., Einhäupl, K.M. (eds) Optical Imaging of Brain Function and Metabolism. Advances in Experimental Medicine and Biology, vol 333. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2468-1_19
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DOI: https://doi.org/10.1007/978-1-4899-2468-1_19
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