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Intracellular Ion Concentrations in the Brain: Approaches Towards in Situ Confocal Imaging

  • Chapter
Optical Imaging of Brain Function and Metabolism

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 333))

Abstract

In the central nervous system, regulation of intracellular ion concentrations is of paramount importance for cell function. Intracellular ion concentrations control fundamental cellular processes like gene expression, metabolism, or excitability. Accordingly, disturbances of ion concentrations play a key role in many diseases, particularly in cerebral ischemia. This article focusses on:

  1. 1.

    a brief review of physiological and pathophysiological mechanisms of intracellular pH ([H+]i) and Calcium ([Ca2+]i) regulation,

  2. 2.

    the description of available techniques to reveal intracellular ion concentrations with particular emphasis on optical approaches, and

  3. 3.

    the presentation of first results from a new approach to measure intracellular ion concentrations in acute rat brain slices and in the intact brain with an optical method.

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  1. Department of Neurology, University of Munich, Munich, Germany

    A. Them

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    Ulrich Dirnagl  & Arno Villringer  & 

  2. Humboldt University, Berlin, Germany

    Ulrich Dirnagl  & Karl M. Einhäupl  & 

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Them, A. (1993). Intracellular Ion Concentrations in the Brain: Approaches Towards in Situ Confocal Imaging. In: Dirnagl, U., Villringer, A., Einhäupl, K.M. (eds) Optical Imaging of Brain Function and Metabolism. Advances in Experimental Medicine and Biology, vol 333. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2468-1_14

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