Abstract
In the past decade, the development of genetic capabilities in methylotrophic bacteria has greatly expanded the scope of research in these organisms. Work is now underway to define the molecular mechanisms involved in regulatory phenomena, to manipulate the physiology of the organisms, and to sort out the complex steps involved in C1 metabolism. This chapter will review recent advances in genetic technology as applied to C1 metabolism in the methanol-utilizing bacteria, with emphasis on the information gained concerning the physiology of these organisms.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Allen, L., and Hanson, R., 1985, Construction of broad-host-range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol. J. Bacteriol. 161:955–962.
Anderson, D., and Lidstrom, M., 1988, The moxFG region encodes four polypeptides in the methanol-oxidizing bacterium Methylobacterium sp. strain AM1, J. Bacteriol. 170:2254–2262.
Anderson, D., Morris, C., Nunn, D., Anthony, C., and Lidstrom, M., 1990, Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation, Gene 90:173–176.
Anthony, C., 1982, The Biochemistry of Methylotrophs, Academic Press, London, 382 pp.
Anthony, C., 1986, Bacterial oxidation of methane and methanol, Adv. Microb. Physiol. 27:113–210.
Bastien, C. A., Machlin, S. M., Zhang, Y., Donaldson, K., and Hanson, R. S., 1989. The organization of genes required for the oxidation of methanol to formaldehyde in three type II methylotrophs, Appl. Environ. Microbiol. 55:3124–30.
Biville, F., Turlin, E., and Gasser, F., 1989, Cloning and genetic analysis of six pyrroloquinoline quinone biosynthesis genes in Methylobacterium organophilum DSM760, J. Gen. Microbiol. 135:2917–29.
Bohanon, M., Bastien, C., Yoshida, R., and Hanson, R., 1987, Isolation of auxotrophic mutants of Methylophilus methylotrophus by modified-marker exchange, Appl. Environ. Microbiol. 54:271–273.
Byrom, D., 1984, Host-vector systems for Methylophilus methylotrophus, in: Microbial Growth on C1 Compounds (R. L. Crawford and R. S. Hanson, eds.), ASM Press, Washington, DC, pp. 221–223.
Chistoserdov, A. Y., and Tsygankov, Y. D., 1986, Broad host range vectors derived from an RSF1010: Tnl plasmid, Plasmid 16:161–167.
Chistoserdov, A., Eremashvili, M., Mashko, S., Lapidus, A., Skvortsova, M., and Sterkin, V., 1987, Expression of human interferon F gene in obligate methylotroph Methylobacillus flagellatum KT and Pseudomonas putida (in Russian), Mol. Genet. Microbiol. Virol. 8:36–42.
De Vries, G., 1986, Molecular biology of bacterial methanol oxidation, FEMS Microbiol. Rev. 39:235–258.
Dijkhuizen, L., Harder, W., De Boer, L., Van Boven, A., Clement, W., Bron, S., and Venema, G., 1984, Genetic manipulation of the restricted facultative methylotroph Hyphomicrobium X by the R-plasmid mediated introduction of the Escherichia colipdh genes, Arch. Microbiol. 139:311–318.
Ditta, G., Schmidhauser, T., Yakobson, E., Lu, P., Liang, X., Finlay, D., Guiney, D., and Helinski, D., 1985, Plasmids related to the broad host range vector, pRK290, useful for gene cloning and monitoring gene expression, Plasmid 13:149–153.
Duine, J., Frank, J., and Jongejan, J., 1986, PQQ and quinoprotein enzymes in microbial oxidations, FEMS Microbiol. Rev. 32:165–178.
Ely, B., 1985, Vectors for transposon mutagenesis of non-enteric bacteria, Mol. Gen. Genet. 200:302–304.
Fulton, G., Nunn, D., and Lidstrom, M., 1984, Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph, J. Bacteriol. 160:718–723.
Goosen, N., Horsman, H., Huinen, R., De Groot, A., and Van De Putte, P., 1989, Genes involved in the biosynthesis of PQQ from Acinetobacter calcoaceticus, Ant. van. Leeuw. 56:85–89.
Harms, N., De Vries, G., Maurer, K., Veitkamp, E., and Stouthamer, A., 1985, Isolation and characterization of Paracoccus denitrificans mutants with defects in the metabolism of one-carbon compounds, J. Bacteriol. 164:1064–1070.
Harms, N., De Vries, G., Maurer, K., Hoogendijk, J., and Stouthamer, A., 1987, Isolation and nucleotide sequence of the methanol dehydrogenase structural gene from Paracoccus denitrificans, J. Bacteriol. 169:3969–3975.
Harms, N., Van Spanning, R. J. M., Oltmann, L. F., and Stouthamer, A. H., 1989, Regulation of methanol dehydrogenase synthesis in Paracoccus denitrificans, Ant. van Leeuw. 56:47–50.
Hennam, J. F., Cunningham, A. E., Sharpe, G. S., and Atherton, K. T., 1982, Expression of eukaryotic coding sequences in Methylophilus methylotrophus, Nature 297:80–82.
Holloway, B., 1984, Genetics of methylotrophs, in: Methylotrophs: Microbiology, Biochemistry and Genetics (C. T. Hou, ed.), CRC Press, Boca Raton, FL, pp. 87–106.
Houck, D., Hanners, J., Unkefer, C., Van Kleef, M., and Duine, J., 1989, PQQ; Biosynthetic studies in Methylobacterium AM1 and Hyphomicrobium X using specific 13C labelling and NMR, Ant. van Leeuw. 56:93–99.
Kearney, P., and Holloway, B., 1987, Expression in Escherichia coli and Pseudomonas aeruginosa of Methylophilus methylotrophus AS1 genes carried on prime plasmids, FEMS Microbiol. Lett. 44:7–11.
Kim, Y., and Lidstrom, M., 1989, Plasmid analysis in pink facultative methylotrophic bacteria using a modified acetone-alkaline hydrolysis method, FEMS Microbiol. Lett. 60:125–129.
Kletsova, L., Chibisova, E., and Tsygankov, Y., 1988, Mutants of the obligate methylotroph Methylobacillus flagellatum KT defective in genes of the ribulose monophosphate cycle of formaldehyde fixation, Arch. Microbiol. 149:411–446.
Knauf, V. C., and Nester, E. W., 1982, Wide host range cloning vectors: a cosmid clone bank of Agrobacterium Ti plasmid, Plasmid 8:45–54.
Levering, P. R., Tiesma, L., Woldendrop, J. P., Steensma, M., and Dijkhuizen, L., 1987, Isolation and characterization of mutants of the facultative methylotroph Arthrobacter P1 blocked in one-carbon metabolism, Arch. Microbiol. 146:346–352.
Lidstrom, M., Nunn, D., Anderson, D., Stephens, R., and Haygood, M., 1987, Molecular biology of methanol oxidation, in: Microbial Growth on C-1 Compounds (H. Van Verseveld and J. Duine, eds.), Nijhoff, Amsterdam, pp. 246–254.
Lyon, B., Kearney, P., Sinclair, M., and Holloway, B., 1988, Comparative complementation mapping of Methylophilus spp. using cosmid gene libraries and prime plasmids, J. Gen. Microbiol. 134:123–130.
Machlin, S., and Hanson, R., 1988, Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene, J. Bacteriol. 170:4739–47.
Machlin, S., Tam, P., Bastien, C., and Hanson, R., 1988, Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation, J. Bacteriol. 170:141–148.
Marquardt, R., Wohner, G., and Winnacker, E., 1984, Properties of plasmids from Methylomonas clara, J. Biotechnol. 1:317–320.
Mazodier, P., Biville, F., Turlin, E., and Gasser, F., 1988, Localization of a pyrroloquinoline quinone biosynthesis gene near the methanol dehydrogenase structural gene in Methylobacterium organophilum DSM 760, J. Gen. Microbiol. 134:2513–24.
Metzler, T., Marquardt, R., Prave, P., and Winnacker, E-L., 1988, Characterisation of a promoter from Methylomonas clara, Mol. Gen. Genet. 21:210–214.
Monteiro, M., Typas, M., Moffett, B., and Bainbridge, B., 1982, Isolation and characterization of a high molecular weight plasmid from the obligate methanol-utilising bacterium Methylomonas (Methanomonas) methylovora, FEMS Microbiol. Lett. 15:235–237.
Moore, A. T., Nayudu, M., and Holloway, B. W., 1983, Genetic mapping in Methylophilus methylotrophus AS1, J. Gen. Microbiol. 129:785–799.
Nunn, D., and Anthony, C., 1988, The nucleotide sequence and deduced amino acid sequence of the cytochrome CL gene of Methylobacterium extorquens AM1, a novel class of c-type cytochrome, Biochem. J. 256:673–676.
Nunn, D., and Lidstrom, M., 1986a, Isolation and complementation analysis of 10 methanol oxidation mutant classes and identification of the methanol dehydrogenase structural gene of Methylobacterium sp. strain AM1, J. Bacteriol. 166:581–590.
Nunn, D., and Lidstrom, M., 1986b, Phenotypic characterization of 10 methanol oxidation mutant classes in Methylobacterium sp. strain AM1, J. Bacteriol. 166:591–597.
Nunn, D. N., Day, D., and Anthony, C., 1989, The second subunit of methanol dehydrogenase of Methylobacterium extorquens AM1, Biochem. J. 260:857–862.
O’Connor, M., 1981, Extension of the model concerning linkage of genes coding for C-1 related functions in Methylobacterium organophilum, Appl. Environ. Microbiol. 41:437–441.
O’Connor, M., and Hanson, R., 1978, Linkage relationships between mutants of Methylobacterium organophilum impaired in their ability to grow on one-carbon compounds, J. Gen. Microbiol. 104:105–111.
Ruvkun, G., and Ausubel, F., 1981, A general method for site-directed mutagenesis in prokaryotes, Nature 298:85–88.
Serebrijski, I., Kazakova, S., and Tsygankov, Y., 1989, Construction of Hfr-like donors of the obligate methanol-oxidizing bacterium Methylobacillus flagellatum KT, FEMS Microbiol. Lett. 59:203–206.
Simon, R., Priefer, U., and Puehler, A., 1983, Abroad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria, Biotechnology 1:784–91.
Stephens, R., Haygood, M., and Lidstrom, M., 1988, Identification of putative methanol dehydrogenase (moxF) structural genes in methylotrophs and cloning of moxF genes from Methylococcus capsulatus Bath and Methylomonas albus BG8, J. Bacteriol. 170:2063–69.
Stone, S., and Goodwin, P., 1989, Characterization and complementation of mutants of Methylobacterium AM1 which are defective in C-1 assimilation, J. Gen. Microbiol. 135:227–234.
Tabor, S., and Richardson, C., 1985, A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes, Proc. Natl. Acad. Sci. USA 82:1074–1078.
Tatra, P., and Goodwin, P., 1983, R-plasmid mediated chromosome mobilization in the facultative methylotroph Pseudomonas AM1, J. Gen. Microbiol. 129:2629–32.
Tatra, P., and Goodwin, P., 1985, Mapping of some genes involved in C-1 metabolism in the facultative methylotroph Methylobacterium sp. strain AM1 (Pseudomonas AM1), Arch. Microbiol. 143:169–177.
Tsuji, K., Tsien, H., Hanson, R., De Palma, S., Scholtz, R., and LaRoche, S., 1990, 16s ribosomal RNA sequence analysis for determination of phylogenetic relationship among methylotrophs, J. Gen. Microbiol. 136:1–10.
Tsygankov, Y., and Kazakova, S., 1987, Development of gene transfer systems in Methylobacillus flagellation KT: isolation of auxotrophic mutants, Arch. Microbiol. 149:112–119.
Ueda, S., Kitamoto, N., Tamura, Y., Sakakibara, Y., and Shimizu, S., 1987, Isolation and characterization of a plasmid in a methylotrophic bacterium, J. Ferment. Technol. 65:589–91.
Warner, P., and Higgins, I., 1977, Examination of obligate and facultative methylotrophs for plasmid DNA, FEMS Microbiol. Lett. 1:339–42.
Whitta, S., Sinclair, M., and Holloway, B., 1985, Transposon mutagenesis in Methylobacterium AMI (Pseudomonas AM1), J. Gen. Microbiol. 131:1547–51.
Windass, J., Worsey, M., Pioli, E., Pioli, D., Barth, P., Atherton, K., Dart, E., Byrom, D., Powell, K., and Senior, P., 1980, Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus, Nature 287:396–401.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lidstrom, M.E. (1992). The Genetics and Molecular Biology of Methanol-Utilizing Bacteria. In: Murrell, J.C., Dalton, H. (eds) Methane and Methanol Utilizers. Biotechnology Handbooks, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2338-7_6
Download citation
DOI: https://doi.org/10.1007/978-1-4899-2338-7_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-2340-0
Online ISBN: 978-1-4899-2338-7
eBook Packages: Springer Book Archive