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The Genetics and Molecular Biology of Obligate Methane-Oxidizing Bacteria

  • Chapter
Methane and Methanol Utilizers

Part of the book series: Biotechnology Handbooks ((BTHA,volume 5))

Abstract

A considerable amount of information on the physiology and biochemistry of obligate methane-oxidizing bacteria (methanotrophs) has emerged over the last 20 or so years (Anthony, 1986; Chapter 3, this volume), particularly regarding the metabolism of methane into cell carbon and also nitrogen assimilation. In contrast, the genetics of these organisms is far less well developed, which is in part due to the relatively slow growth of methanotrophs on solid media. To carry out genetic studies on microorganisms, it is necessary to develop the capabilities for mutant isolation and gene transfer. Traditionally, methanotrophs have proved refractory to most forms of conventional genetic analysis. However, with the advent of gene-cloning technology and the development of numerous broad-host-range cloning vectors, a number of significant advances are being made in the molecular genetics of methanotrophs. In this chapter, I will review the current literature on the mutagenesis, plasmid, bacteriophage, and gene transfer studies carried out on methanotrophs and then concentrate on the more recent developments in molecular genetics related to Cl-specific genes, such as those encoding for methanol dehydrogenase and methane monooxygenase, and also the molecular biology of nitrogen assimilation genes, which has served as a model system in which to develop genetic techniques for this unique group of bacteria.

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  1. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, England

    J. Colin Murrell

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Murrell, J.C. (1992). The Genetics and Molecular Biology of Obligate Methane-Oxidizing Bacteria. In: Murrell, J.C., Dalton, H. (eds) Methane and Methanol Utilizers. Biotechnology Handbooks, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2338-7_4

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