Summary
Aspects of the use of overpressured layer chromatography (OPLC) as an instrumentalised version of planar liquid chromatography, suitable for both analytical and preparative off-line and on-line separations, are described.
For example the eluent strength and solvent composition considerably influence the applicability of off-line OPLC owing to the demixing of the solvent during chromatography. The disturbing effect due to this demixing phenomenon can be reduced or eliminated if the differences in solvent strength between the eluent constitutents are kept to a minimum.
There is a strong correlation between dry and wet OPLC separations regarding retention characteristics, which offers a new prospect for the modelling of high performance liquid chromatography.
In the case of preparative on-line separation and detection prewetting the stationary phase from outlet side can eliminate disturbances which otherwise adversly affect detection.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
E. Tyihák and G. Held, in: “Progress In TLC And Related Methods,” Vol. II, A. Niederwieser and G. Pataki, ed., Ann Arbor sci. Publ., Ann Arbor, Mich., (1971).
E. Tyihák, E. Mincsovics and H. Kalász, J.Chromatogr., 174:75 (1979).
E. Mincsovics, E. Tyihák, and H. Kalász, J.Chromatogr., 191:293 (1980).
H. Kalász, J. Nagy, E. Tyihák, and E. Mincsovics, J.Liquid Chromatogr., 3:845 (1980).
E. Tyihák, E. Mincsovics and F. Körmendi, Hung.Sci.Instru., 55:33 (1983).
E. Tyihák, T. J. Székely, and E. Mincsovics, in: Proc.2nd.Int.Symp.on Instrumental HPTLC, R. E. Kaiser, ed., Interlaken, Inst. for Chromatography, Bad Dürkheim, 159 (1982).
E. Tyihák and E. Mincsovics, in: Proc 3rd. Int. Symp. On Instrumental HPTLC, R. E. Kaiser, ed., Würzburg, p. 442 (1985).
E. Tyihák, E. Mincsovics, H. Kalász, and J. Nagy, J.Chromatogr., 211:45 (1981).
H. F. Hauck and W. Jost, J. Chromatogr., 262:113 (1983).
Zs. Fatér and E. Mincsovics, J. Chromatogr., 298:534 (1984).
E. Mincsovics, E. Tyihák and A. M. Siouffi, in: Proc.Int.Symp.TLC with Special Emphasis on OPLC, E. Tyihák, ed., Szeged (1984), Labor MIM Company, Budapest, p. 251.
L. R. Snyder and J. J. Kirkland, Introduction to Modem Liquid Chromatography (2nd. Ed.), J. Wiley and Sons Inc., New York (1979).
G. Guiochon, J.Chromatogr., 185:3 (1979).
N. Mellor, Chromatographia, 16:359 (1982).
A. Niederwieser and M. Brenner, Experientia, 21:50 (1965).
K. Dallenbach-Toelke, Sz. Nyiredy, B. Meier and O. Sticher, J. Chromatogr., 365:63 (1986).
L. R. Snyder, Principles of Adsorption Chromatography, Marcel Dekker, New York, Ch. 8. (1986).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer Science+Business Media New York
About this chapter
Cite this chapter
Mincsovics, E., Tyihák, E. (1988). Analytical and Preparative Overpressured Layer Chromatography (OPLC). In: Dallas, F.A.A., Read, H., Ruane, R.J., Wilson, I.D. (eds) Recent Advances in Thin-Layer Chromatography. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2221-2_6
Download citation
DOI: https://doi.org/10.1007/978-1-4899-2221-2_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-2223-6
Online ISBN: 978-1-4899-2221-2
eBook Packages: Springer Book Archive