Summary
A new analytical rotation planar chromatographic method, micro chamber rotation planar chromatography (M-RPC), is described in detail and compared with ultra-micro chamber rotation planar chromatography (U-RPC). The principle and possibilities of these forced-flow methods are discussed with respect to the chamber types, applicable stationary and mobile phases, development modes and operating parameters.
In the micro chamber the vapor space is variable whilst in the ultra-micro chamber it is practically eliminated; therefore, M-RPC resembles a saturated system while U-RPC is more akin to an unsaturated system. The mobile phase has to be optimized accordingly and can then be transferred from thin-layer chromatography (TLC) preassays to M-RPC or U-RPC without modification. All commercially available stationary phases can be used for the separations using either TLC or high-performance (HP) TLC quality. Circular, anticircular, and linear development modes and combinations thereof are possible using both methods.
The advantages and disadvantages of both M-RPC and U-RPC are discussed and compared with other planar chromatographic methods.
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Nyiredy, S., Dallenbach-Toelke, K., Sticher, O. (1988). Analytical Rotation Planar Chromatography. In: Dallas, F.A.A., Read, H., Ruane, R.J., Wilson, I.D. (eds) Recent Advances in Thin-Layer Chromatography. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2221-2_5
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DOI: https://doi.org/10.1007/978-1-4899-2221-2_5
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