Abstract
Exposure to genotoxic carcinogens results in the formation of covalently bound adducts between the genotoxin and DNA, which may cause mutation and cytogenetic alterations. The dose of the genotoxic carcinogenic compounds involved in the exposure may be monitored by quantitative analysis of DNA adducts, and these data may also be used as an indicator of genotoxic risk. The analytical techniques required for adduct measurements need to be of exceptional sensitivity and include 32P-postlabelling, mass spectrometry and immunoassay. Exposure to alkylating carcinogens may also be monitored by measurement of adducts formed with amino acids in haemoglobin (Hb). Since, for a variety of compounds, the extent of protein-adduct formation relates quantitatively with that of DNA adducts, measurement of the former may indicate the biologically-effective dose of the compound received.
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Farmer, P.B. (1993). Biomarkers as Molecular Dosimeters of Genotoxic Substances. In: Travis, C.C. (eds) Use of Biomarkers in Assessing Health and Environmental Impacts of Chemical Pollutants. NATO ASI Series, vol 250. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2052-2_6
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DOI: https://doi.org/10.1007/978-1-4899-2052-2_6
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