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Genetics of the Penicillia

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Penicillium and Acremonium

Part of the book series: Biotechnology Handbooks ((BTHA,volume 1))

Abstract

The genus Penicillium contains species that lack a demonstrable sexual or perfect stage as well as those with one. Many species of Penicillium have actual and potential applications in biotechnology (see Chapter 1), but much interest has focused on the production of secondary metabolites, especially antibiotics. For example, the production of penicillin by the imperfect species P. chrysogenum has been intensively developed as an industrial process for the last 40 years (Queener and Schwartz, 1979) (see also Chapter 5). However, within this time, no clear understanding of the genetic basis for the regulation of penicillin production has emerged. While this status undoubtedly reflects the absence of classic genetic studies via the sexual cycle, another significant contributing factor is the complexity of secondary-metabolite synthesis in general.

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References

  • Anné, J., 1977, Somatic hybridisation between Penicillium species after induced fusion of their protoplasts, Agricultura 25:1–117.

    Google Scholar 

  • Anné, J., 1983, Protoplasts of filamentous fungi in genetics and metabolite production, in Protoplasts 1983: Lecture Proceedings (I. Potrykus, C. T. Harms, A. Hinner, R. Hutter, P.J. King, and R. D. Shillito, eds.), Birkhauser Verlag, Basel, Stuttgart, and New York, pp. 167–178.

    Google Scholar 

  • Anne, J., and Peberdy, J. F., 1975, Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions, Arch. Microbiol. 105:201–205.

    Article  PubMed  CAS  Google Scholar 

  • Anné, J., and Peberdy, J. F., 1976, Induced fusion of fungal protoplasts following treatment with polyethylene glycol, J. Gen. Microbiol. 92:413–417.

    Article  PubMed  Google Scholar 

  • Auerbach, C., 1976, Mutation Research: Problems, Results, Perspectives, Chapman Hall, London.

    Google Scholar 

  • Ball, C. 1971, Haploidisation analysis in Penicillium chrysogenum, J. Gen. Microbiol. 66:63–69.

    Article  PubMed  CAS  Google Scholar 

  • Ball, C., 1982, Genetic approaches to overproduction of beta-lactam antibiotics in eukaryotes, in: Overproduction of Microbial Products (V. Krumphanzl, B. Sikyta, and Z. Vanek, eds.), Academic Press, New York and London, pp. 515–532.

    Google Scholar 

  • Ball, C., 1983, Protoplast fusion in commercially important microorganisms, in: Bioactive Microbial Products: Development and Production (L.J. Nisbet and D.J. Winstanley, eds.), Academic Press, New York and London, pp. 19–32.

    Google Scholar 

  • Ball, C., and Azevedo, J. L., 1976, Genetic instability in parasexual fungi, in: Proceedings of the Second International Symposium on the Genetics of Industrial Microorganisms (K. D. Macdonald, ed.), Academic Press, New York and London, pp. 243–251.

    Google Scholar 

  • Ballance, D. J., Buxton, F. P., and Turner, G., 1983, Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa, Biochem. Biophys. Res. Commun. 112:284–289.

    Article  PubMed  CAS  Google Scholar 

  • Birkett, J. A., and Rowlands, R. T., 1981, Chlorate resistance and nitrate assimilation in industrial strains of Penicillium chrysogenum, J. Gen. Microbiol. 123:281–285.

    CAS  Google Scholar 

  • Calos, M. P., and Miller, J. H., 1980, Transposable elements, Cell 20:579–595.

    Article  PubMed  CAS  Google Scholar 

  • Cummings, D. J., Belcour, L., and Grandeschamp, C., 1979, Mitochondrial DNA from Podospora anserina, Mol. Gen. Genet. 171:229–238.

    Article  PubMed  CAS  Google Scholar 

  • Ditchburn, P., Giddings, B., and Macdonald, F. D., 1974, Rapid screening for the isolation of mutants of Aspergillus nidulans with increased penicillin yield, J. Appl. Bacteriol. 37:515–523.

    Article  PubMed  CAS  Google Scholar 

  • Edwards, G. F. St. L., Normanseil, I. D., and Holt, G., 1975, Benlate induced haploidisation in diploid strains of Aspergillus nidulans and Penicillium chrysogenum, Aspergillus Newslett. 12:15.

    Google Scholar 

  • Elander, R. P., 1981, Strain improvement programmes in antibiotic producing microorganisms: Present and future strategies, in Advances in Biotechnology, Vol. 1 (M. Moo-Young, C. W. Robinson, and C. Vezina, eds.), Pergamon Press, Oxford, pp. 3–8.

    Google Scholar 

  • Elander, R. P., 1982, Traditional versus current approaches to the genetic improvement of microbial strains, Overproduction of Microbial Products (V. Krumphanzl, B. Sikyta, and Z. Vanek, eds.), Academic Press, New York and London, pp. 353–370.

    Google Scholar 

  • Fawcett, P. A., Loder, P. B., Duncan, M. J., Beesley, T. J., and Abraham, E. P., 1973, Formation and properties of protoplasts from antibiotic producing strains of Penicillium chrysogenum and Cephalosporium acremonium, J. Gen. Microbiol. 79:293–309.

    Article  PubMed  CAS  Google Scholar 

  • Fincham, J. R. S., Day, P. R., and Radford, A., 1979, Botanical Monographs, Vol. 4, Fungal Genetics, Blackwell, Oxford.

    Google Scholar 

  • Fjeld, A., and Stromnaes, O., 1966, The parasexual cycle and linkage groups in Penicillium expansum, Hereditas 54:389–403.

    Article  PubMed  CAS  Google Scholar 

  • Glickman, B., and Radman, M., 1980, E. coli mutator mutants deficient in methylation instructed DNA mismatch correction, Proc. Natl. Acad. Sci. U.S.A. 77:1063–1066.

    Article  PubMed  CAS  Google Scholar 

  • Grant, D. M., Lambowitz, A. M., Rambosek, J. A., and Kinsey, J. A., 1984, Transformation of Neurospora crassa with recombinant plasmids containing the cloned glutamate dehydrogenase (am) gene: Evidence for autonomous replication of the transformation plasmid, Mol. Cell. Biol. 4:2401–2051.

    Google Scholar 

  • Gupalo, I. D., Surkov, V. V., Tikhonenko, T. I., Ergorov, A. A., and Parjenov, N. N., 1978, DNA from Penicillium chrysogenum, Vestn. Mosk. Univ. Ser. 16:62–66.

    Google Scholar 

  • Holt, G., and Saunders, G., 1986, Genetic modification of industrial microorganisms, in Comprehensive Biotechnology, Vol. 1 (M. Moo-Young, ed.), Pergamon Press, Oxford, pp. 51–76.

    Google Scholar 

  • Joglekar, A. V., and Karanth, N. G., 1984, Studies on cellulase production by a mutant: Penicillium funiculosum UV-49, Biotechnol. Bioeng. 26:1079–1084.

    Article  PubMed  CAS  Google Scholar 

  • Kimball, R. F., 1978, The relation of repair phenomena to mutation induction in bacteria, Mutat. Res. 55:85–120.

    Article  PubMed  CAS  Google Scholar 

  • Lemontt, J. F., and Lair, S. V., 1982, Plate assay for chemical and radiation induced mutagenesis of an1 in yeast as a function of post treatment DNA replication: The effect of rad6-1, Mutat. Res. 93:339–352.

    Article  CAS  Google Scholar 

  • Macdonald, K. D., 1963, The selection of auxotrophs of P. chrysogenum with nystatin, Genet. Res. 11:327–330.

    Article  Google Scholar 

  • Macdonald, K. D., and Holt, G., 1976, Genetics of biosynthesis and overproduction of penicillin, Sci. Prog. (Oxford) 63:547–573.

    CAS  Google Scholar 

  • Macdonald, K. D., Hutchinson, J. M., and Gillet, W. A., 1965, Heterozygous diploids of Penicillium chrysogenum and their segregation pattern, Genetica 36:378–397.

    Article  PubMed  Google Scholar 

  • Mandai, S. K., and Chatterjee, S. P., 1985, Improved production of calcium gluconate by mutants of Penicillium funiculosum, Curr. Sci. 54:149–150.

    Google Scholar 

  • Maniatis, T., Fritsch, E. F., and Sambrook, J., 1982, Molecular Cloning—A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  • Mellon, F. M., Peberdy, J. F., and Macdonald, K. D., 1983, Hybridisation of Penicillium chrysogenum and Penicillium baarnense by protoplast fusion: Genetic and biochemical analysis, in: Protoplasts 1983: Lecture Proceedings (I. Potrykus, C. T. Harms, A. Hinner, R. Hutter, P. J. King, and R. D. Shillito, eds.), Birkhauser Verlag, Basel, Stuttgart, and New York, pp. 310–311.

    Google Scholar 

  • Mohamed, Z. S., Todorova-Dragonova, R. W., and Luckner, M., 1984, Nuclear inheritance of the biosynthesis of cyclopenin and cyclopenol in P. cyclopium, Z. Allg. Mikrobiol. 24:615–618.

    Article  PubMed  CAS  Google Scholar 

  • Morris, N. R., Kirsch, D. R., and Oakley, B. R., 1982, Molecular and genetic methods for studying mitosis and spindle proteins in Aspergillus nidulans, Methods Cell Biol. 25:107–130.

    Article  PubMed  CAS  Google Scholar 

  • Normansell, I. D., and Holt, G., 1978, Viruses in strains of Penicillium chrysogenum impaired in penicillin biosynthesis, Mycovirus Newslett. 6:15.

    Google Scholar 

  • Normansell, P. J. M., Normansell, I. D., and Holt, G., 1979, Genetic and biochemical studies of mutants of Penicillium chrysogenum impaired in penicillin biosynthesis, J. Gen. Microbiol. 112:113–126.

    Article  PubMed  CAS  Google Scholar 

  • Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D., and Bufton, A. W.J., 1953, The genetics of Aspergillus nidulans, Adv. Genet. 5:141–238.

    Article  PubMed  CAS  Google Scholar 

  • Queener, S., and Schwartz, R., 1979, Penicillins: Biosynthetic and semi-synthetic, Economic Microbiology, Vol. 3 (A. H. Rose, ed.), Academic Press, New York and London, pp. 35–122.

    Google Scholar 

  • Queener, S. W., Wilkerson, S. G., and Nash, C. H., 1982, Sterol content and titre of mycophenolic acid in polyene antibiotic resistant mutants of Penicillium stoloniferum, in: Overproduction of Microbial Products (V. Krumphanzl, B. Sikyta, and Z. Vanek, eds.), Academic Press, New York and London, pp. 535–548.

    Google Scholar 

  • Rogers, S. D., and Holt, G., 1984a, DNA repair and mutagenesis in Penicillium chrysogenum, Trans. Biochem. Soc. 12:646–647.

    CAS  Google Scholar 

  • Rogers, S. D., Rogers, M. E., Saunders, G., and Holt, G., 1986, Isolation of mutants sensitive to 2-aminopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum, Curr. Genet. 10:557–560.

    Article  PubMed  CAS  Google Scholar 

  • Rogers, S. D., and Holt, G., 1984b, DNA repair deficient mutants from Penicillium chrysogenum, J. Appl. Microbiol. Biotechnol. 20:251–255.

    CAS  Google Scholar 

  • Rowlands, R. T., 1984a, Industrial strain improvement: Mutagenesis and random screening procedures, Enzyme Microbiol. Technol. 6:3–10.

    Article  CAS  Google Scholar 

  • Rowlands, R. T., 1984b, Industrial strain improvement: Rational screens and genetic recombination techniques, Enzyme Microbiol. Technol. 6:290–300.

    Article  CAS  Google Scholar 

  • Rowlands, R. T., and Normansell, I. D., 1983, Current strategies in industrial selection, in Bioactive Microbial Products (L. J. Nisbett and D. J. Winstanley, eds.), Academic Press, New York and London, pp. 1–18.

    Google Scholar 

  • Saunders, G., and Holt, G., 1982, Far ultra-violet light sensitive mutants of Streptomyces clavuligerus, J. Gen. Microbiol. 128:381–385.

    Google Scholar 

  • Saunders, G., Allsop, A. E., and Holt, G., 1980, Modern developments in mutagenesis, J. Chem. Technol. Biotechnol. 32:354–364.

    Article  Google Scholar 

  • Saunders, G., Rogers, M. E., Adlard, M. W., and Holt, G., 1984, Chromatographic resolution of nucleic acids extracted from Penicillium chrysogenum, Mol. Gen. Genet. 194:343–346.

    Article  CAS  Google Scholar 

  • Sermonti, G., 1956, Complementary genes which affect penicillin yields, J. Gen. Microbiol. 15:599–608.

    Article  PubMed  CAS  Google Scholar 

  • Smith, T. M., and Holt, G., 1984, Cloning of DNA from P. chrysogenum, Trans. Biochem. Soc. 12:645–646.

    CAS  Google Scholar 

  • Stohl, L. L., and Lambowitz, A. M., 1983, Construction of a shuttle vector for the filamentous fungus Neurospora crassa, Proc. Natl. Acad. Sci. U.S.A. 80:1058–1062.

    Article  PubMed  CAS  Google Scholar 

  • Stromnaes, O., Garba, E. D., and Beraha, L., 1964, Genetics of phytopathogenic fungi. IX. Heterokaryosis and the parasexual cycle in Penicillium italicum and Penicillium digitatum, Can. J. Bot. 42:423–427.

    Article  Google Scholar 

  • Timmis, K. N., 1981, Gene manipulation in vitro, in: Genetics as a Tool in Microbiology (S. W. Glover and D. A. Hopwood, eds.), Cambridge University Press, Cambridge, pp. 49–109.

    Google Scholar 

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Author information

Authors and Affiliations

  1. School of Biotechnology, Faculty of Engineering and Science, The Polytechnic of Central London, London, W1M 8JS, England

    G. Saunders

  2. Biological Laboratory, University of Kent at Canterbury, Kent, CT2 TNJ, England

    G. Holt

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  1. G. Saunders
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  2. G. Holt
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Editors and Affiliations

  1. University of Nottingham, Nottingham, England

    John F. Peberdy

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© 1987 Springer Science+Business Media New York

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Saunders, G., Holt, G. (1987). Genetics of the Penicillia . In: Peberdy, J.F. (eds) Penicillium and Acremonium . Biotechnology Handbooks, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1986-1_3

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  • DOI: https://doi.org/10.1007/978-1-4899-1986-1_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1988-5

  • Online ISBN: 978-1-4899-1986-1

  • eBook Packages: Springer Book Archive

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