Abstract
In the last decade biosubstances such as enzymes and antibodies have been used in conjunction with electrochemical sensoring devices to form bioselective sensors (biosensors), which include enzyme sensors, microbial sensors, and immunosensors (Aizawa, 1983; Suzuki, 1984). An enzyme sensor is the union of an enzyme, that is a biological catalyst which acts sensitively and specifically to almost all organic and inorganic componds in nature, with an electrochemical sensor. The result is a sensor which is useful for the assay of organic and inorganic compounds in such a manner as simple as a pH measurement with a glass electrode. Since an enzyme is watersoluble, it is immobilized (insolubilized) on solid matrix at or near the sensing device surface to serve as biocatalyst for specific molecular recognition. Due to marked progress in enzyme immobilization techniques, many enzyme sensors have been developed for ionic and low molecular weight compounds and evaluated particularly in clinical fields.
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References
Aizawa, M., Kato, S. and Suzuki, S., 1977, Immunoresponsive membrane, J. Membrane Sci. 2: 125–132.
Aizawa, M., Suzuki, S., Nagamura, Y., Shinohara, R. and Ishiguro, I., 1977, An immunosensor for specific protein, Chem. Lett, 1977: 779–782.
Aizawa, M., Kato, S., Suzuki, S., Nagamura, Y. and Ishiguro, I., 1977, Immunoresponsive membrane H., Kobunshi Ronbunshu, 34: 813–818.
Aizawa, M., Suzuki, S., Nagamura, Y., Shinihara, R. and Ishiguro, I., 1979, An immunosensor for syphilis, J. Solid-Phase Biochem. 4: 25–31.
Aizawa, M., Kato, S. and Suzuki, S., 1980, Electrochemical typing of blood using affinity membranes, J. Membrane Sci. 7: 1–10.
Aizawa, M. and Suzuki, S., 1982, Chemical amplification in biosensors, Jap. J. Appl. Phys. 21(21-1): 219–223.
Aizawa, M., 1983, Molecular recognition and chemical amplification of biosensors, in: “Chemical Sensors,” T. Seiyama, et al., eds., Kodansha, Tokyo. Janata, J., 1975, Immunoelectrode, J. Am. Chem. Soc. 97: 2914-2915
Kobatake, Y., Takeguchi, N., Toyoshima, Y. and Fujita, H., 1965, Studies of membrane phenomina, J. Phys. Chem. 69: 3981–3987.
Suzuki, S., ed., 1984, “Biosensors,” Kodansha, Tokyo.
Yamamoto, Y., Nagasawa, Y., Sawai, M., Suda, T. and Tsubomura, H., 1978, Potentiometric investigations of antigen-antibody and enzyme-enzyme inhibitor reactions using chemically modified metal electrodes, J. Immunol. Methods 22: 309–315.
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© 1987 Springer Science+Business Media New York
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Suzuki, S., Aizawa, M. (1987). Immuno-Potentiometric Sensors with Antigen Coated Membrane. In: Ngo, T.T. (eds) Electrochemical Sensors in Immunological Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1974-8_4
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DOI: https://doi.org/10.1007/978-1-4899-1974-8_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-1976-2
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