Skip to main content

Reversed Micelles for Protein Purification

  • Chapter

Abstract

Reversed micelles are aggregates of surfactant molecules containing an inner core of water molecules, dispersed in a continuous organic solvent medium. These systems are optically transparent and thermodynamically stable. The considerable biotechnological potential of these systems is derived principally from the ability of the water droplets to dissolve enzymes, without loss of activity, in much the same way as bulk water does. The use of these systems in the biotransformation of apolar compounds present in the bulk oil phase has been described in a number of publications (Luisi, 1985; Martinek and Semenov, 1981; Hilhorst et al., 1983; Laane et al., 1987; Fletcher and Robinson, 1985).

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Dahuron, L., and Cussler, E. L., 1988, Protein extraction with hollow fibers, AIChE J. 34:130–136.

    Article  CAS  Google Scholar 

  • Dekker, M., Van’t Riet, K., Weijers, S. R., Baltussen, J. W. A., Laane, C., and Bijsterbosch, B. H., 1986, Enzyme recovery by liquid-liquid extraction using reversed micelles, Chem. Eng. J. 33:B27–B33.

    Article  Google Scholar 

  • Dekker, M., Van’t Riet, K., Wijnans, J. M. G. M., Baltussen, J. W. A., Bijsterbosch, B. H., and Laane, C., 1987a, Membrane based liquid/liquid extractions of enzymes using reversed micelles, in: Proceedings of the International Congress on Membranes and Membrane Processes (ICOM), Tokyo, pp. 793-794.

    Google Scholar 

  • Dekker, M., Van’t Riet, K., Baltussen, J. W. A., Bijsterbosch, B. H., Hilhorst, R., and Laane, C., 1987b, Reversed micellar extraction of enzymes: Investigations on the distribution behaviour and extraction efficiency of α-Amylase, in: Proceedings of the 4th European Congress on Biotechnology, Elsevier, Amsterdam, Vol. II, pp. 507–510.

    Google Scholar 

  • Dekker, M., Van’t Riet, K., Bijsterbosch, B. H., Wolbert, R. B. G., and Hilhorst, R., 1989, Modeling and optimization of the reversed micellar extractions of α-amylase, AIChE J. 35:321–324.

    Article  CAS  Google Scholar 

  • Dekker, M., 1990a, Enzyme recovery using reversed micelles, Ph.D. Thesis, Agricultural University Wageningen, The Netherlands.

    Google Scholar 

  • Dekker, M., Van’t Riet, K., Bijsterbosch, B. H., Fijneman, P., and Hilhorst, R., 1990b, Mass transfer rate of protein extraction with reversed micelles, Chem. Eng. Sci. 45:2949–2957.

    Article  CAS  Google Scholar 

  • Dekker, M., Koenen, P. H. M., and Van’t Riet, K., 1991a, Reversed micellar—membrane—Extraction of Enzymes, Food Bioproduct. Process. Trans. Inst. Chem. Eng. 69(C):54–58.

    Google Scholar 

  • Dekker, M., Van’t Riet, K., Van Der Pol, J. J., Baltussen, J. W. A., Hilhorst, R., and Bijsterbosch, B. H., 1991b, Temperature effect on the reversed micellar extraction of enzymes, Chem. Eng. J. 46:B69–B74.

    Article  CAS  Google Scholar 

  • Fletcher, P. D. I., and Robinson, B., 1985, The partitioning of proteins between water-in-oil microemulsions and conjugate aqueous phases, J. Chem. Soc. Faraday Trans. I 81:2667–2679.

    Article  CAS  Google Scholar 

  • Göklen, K. E., and Hatton, T. A., 1985, Protein extraction using reversed micelles, Biotechnol. Prog. 1:69–74.

    Article  Google Scholar 

  • Göklen, K. E., and Hatton, T. A., 1987, Liquid-liquid extraction of low molecular weight proteins by selective solubilization in reversed micelles, Sep. Sci. Technol. 22:831–841.

    Article  Google Scholar 

  • Hilhorst, R., Laane, C., and Veeger, C., 1983, Enzymatic conversion of apolar compounds in organic media using an NADH-regenerating system and dihydrogen as reductant, FEBS Lett. 159:225–228.

    Article  CAS  Google Scholar 

  • Kahlweit, M., and Strey, R., 1985, Phase behaviour of ternary systems of the type water-oil-nonionic amphiphiles (microemulsions), Angew. Chem. Int. Ed. Engl. 24:655–669.

    Article  Google Scholar 

  • Laane, C., Hilhorst, R., and Veeger, C., 1987, Design of reversed micellar media for the enzymatic synthesis of apolar compounds, Methods Enzymol. 136:229–316.

    Google Scholar 

  • Luisi, P. L., 1985, Enzymes as guest molecules in inverse micelles, Angew. Chem. Int. Ed. Engl. 24:439–460.

    Article  Google Scholar 

  • Luisi, P. L., Bonner, F. J., Pellegrini, A., Wiget, P., and Wolf, R., 1979, Micellar solubilization of proteins in aprotic solvents and their spectroscopic characterization, Helv. Chim. Acta 62:740–753.

    Article  CAS  Google Scholar 

  • Martinek, K., and Semenov, A. N., 1981, Enzymes in organic synthesis: Physico-chemical means of increasing the yield of end product in biocatalysis, J. Appl. Biochem. 3:93–126.

    CAS  Google Scholar 

  • Rahaman, R. S., Chee, J. Y., Cabral, J. M. S., and Hatton, T. A., 1988, Recovery of an extracellular alkaline protease from whole fermentation broth using reversed micelles, Biotechnol. Prog. 4(4):218–224.

    Article  CAS  Google Scholar 

  • Van’t Riet, K., and Dekker, M., 1984, Preliminary investigations on an enzyme liquid-liquid extraction process, in: Proceedings of the 3rd European Congress on Biotechnology, Elsevier, Amsterdam, Vol. III, pp. 541–544.

    Google Scholar 

  • Wolbert, R. B. G., Hilhorst, R., Voskuilen, G., Nachtegaal, H., Dekker, M., Van’t Riet, K., and Bijsterbosch, B. H., 1989, Protein transfer from an aqueous phase into reversed micelles: The effect of protein size and charge distribution, Eur. J. Biochem. 184:627–633.

    Article  PubMed  CAS  Google Scholar 

  • Woll, J. M., Dillon, A. S., Rahaman, R. S., and Hatton, T. A., 1987, Protein separations using reversed micelles, in: Protein Purification: Micro to Macro (R. Burgess, ed.), Alan R. Liss, New York, pp. 117–130.

    Google Scholar 

  • Woll, J. M., Hatton, T. A., and Yarmush, M. L., 1989, Bioaffinity separation using reversed micellar extractions, Biotechnol. Prog. 5:57–62.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer Science+Business Media New York

About this chapter

Cite this chapter

Dekker, M. (1993). Reversed Micelles for Protein Purification. In: Ngo, T.T. (eds) Molecular Interactions in Bioseparations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1872-7_35

Download citation

  • DOI: https://doi.org/10.1007/978-1-4899-1872-7_35

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1874-1

  • Online ISBN: 978-1-4899-1872-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics