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Affinity Chromatography on Inorganic Support Materials

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Book cover Molecular Interactions in Bioseparations

Abstract

Inorganic support materials have been used for chromatography for over 75 years. However, it has only been within the last 25 years that these materials have been applied to affinity separations in which the affinity ligand is covalently bonded to the support. This chapter will cover the application of inorganic silicas and metal oxides for coupling to ligands of various types for the isolation and purification of antigens, antibodies, enzymes, nucleic acids, and related species. Ion chromatography or the use of hydrocarbon-bonded phases for protein isolations will not be covered here, nor will the application of ion exchange as a separation and purification method on inorganic supports.

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  1. Biotechnology Division, Chemical Sciences and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, 20899, USA

    Howard H. Weetall

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  1. Howard H. Weetall
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  1. Department of Developmental and Cell Biology, University of California, Irvine, California, USA

    That T. Ngo

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Weetall, H.H. (1993). Affinity Chromatography on Inorganic Support Materials. In: Ngo, T.T. (eds) Molecular Interactions in Bioseparations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1872-7_3

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  • DOI: https://doi.org/10.1007/978-1-4899-1872-7_3

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