Abstract
It seems appropriate to subdivide the field of affinity chromatography into a bioselective and a chemoselective one. Bioselective affinity chromatography is based upon defined biological-type recognition systems between immobilized ligands and biomolecules (proteins, nucleic acids, low-molecular-weight biomolecules, etc.). The interaction between the ligand and the biomolecule is of a rather complex nature involving different types of noncovalent interactions (such as van der Waals forces, hydrophobic interactions, hydrogen bonding, dipole-dipole interactions, charge-transfer interactions, and Coulombic interactions). For a highly selective interaction, the orientation in space of the functional groups acting as binding sites is an important factor. This orientation facilitates a highly cooperative combination of interactions. In addition to the orientation of the functional groups, an exact steric fit of the two complementary compounds considerably improves selectivity (shape selectivity).
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Wulff, G. (1993). Biorecognition in Molecularly Imprinted Polymers. In: Ngo, T.T. (eds) Molecular Interactions in Bioseparations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1872-7_23
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DOI: https://doi.org/10.1007/978-1-4899-1872-7_23
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