Skip to main content

A Versatile Time-Resolved Laser Scanning Confocal Microscope

  • Chapter
Fluorescence Microscopy and Fluorescent Probes

Abstract

Confocal microscopes are quickly becoming a de facto standard piece of equipment for the investigation of fluorescence distribution in microdomains, particularly in the medical sciences [1,2]. However, their complexity and the requirement for fast, highly linear scanners, high quality optical elements and at least one laser source means that the cost of such apparatus tends to be prohibitive.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. S. Paddock, Proc. Soc.Exp. Biol. Med. 198, 3 (1991)

    Google Scholar 

  2. J.G. White, W.B. Amos, M. Fordham, Journal of Cell Biol. ogy 105, 41 (1987)

    CAS  Google Scholar 

  3. Ambroz, M., MacRobert, A.J., Morgan, J., Rumbles, G., Foley, M.S.C., and Phillips, D.J. Photochem. Photobiol. B. Biol. 22, 105-117, (1994)

    Google Scholar 

  4. D. O’Connor and D. Phillips Time Correlated Single Photon Counting, Academic Press, London (1984)

    Google Scholar 

  5. M. Minsky, U.S. Patent #3013467. Microscopy Apparatus (1957)

    Google Scholar 

  6. M. Minsky, Scanning 10 128 (1988)

    Article  Google Scholar 

  7. M. Gu, C.J.R. Sheppard, X. Gan, Journal of the Optical Society of America 8 1755 (1991)

    Article  Google Scholar 

  8. S. Kimura, T. Wilson, Applied Optics 30 2143 (1991)

    Article  PubMed  CAS  Google Scholar 

  9. J. Benschop, Appl. Optics 30 1179 (1991)

    Article  CAS  Google Scholar 

  10. P.M. Delaney, M. Harris, R. G. King, Clinical and Experimental Pharmacology and Physiology 20 197–198 (1993)

    Article  PubMed  CAS  Google Scholar 

  11. Ambroz, M., Beeby, A., MacRobert, A.J., Simpson, M.S.C., Svenson, R. and Phillips, D. J. Photochem. Photobiol. B. Biol. 9, 87–95 (1991)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer Science+Business Media New York

About this chapter

Cite this chapter

Eigenbrot, I.V., Crystall, B., Phillips, D. (1996). A Versatile Time-Resolved Laser Scanning Confocal Microscope. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4899-1866-6_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1868-0

  • Online ISBN: 978-1-4899-1866-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics