Abstract
Time-resolving fluorescence imaging has become a valuable tool in photobiology and medical diagnostics1. Its techniques include two-dimensional laser scanning microscopy with signal detection in pre-selected time windows2 as well as fluorescence microscopy and spectroscopy using pulsed laser excitation and time-gated image intensifying units3–6. In addition, experimental systems working in the frequency domain become more and more promising. In this case, the excitation light and the voltage of the image intensifier are modulated with a certain phase shift. This shift may be selected such that the detection of fluorescent components with specific lifetimes is enhanced, whereas other components are suppressed. The method has been applied to measure the distribution of specific markers7, 8 and of natural fluorophores9 in various cellular environments.
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Schneckenburger, H., Gschwend, M.H., König, K., Sailer, R., Strauß, W.S.L. (1996). Fluorescence Lifetime Imaging and Spectroscopy in Photobiology and Photomedicine. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_8
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DOI: https://doi.org/10.1007/978-1-4899-1866-6_8
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