Abstract
Macrophages are suggested to play a key role in the pathogenesis of atherosclerosis in hypercholesterolemic rabbits. We characterized macrophages in the cardiovascular system of normo- and hypercholesterolemic rabbits by use of quantitative fluorescence immunohistochemistry. Colocalization of RAM11-, MHC Class I- and II-, MAC-1-, and manganese Superoxide dismutase (MnSOD)-immunoreactivities could be demonstrated in a subpopulation of macrophages using differently fluorescent labelled secondary antibodies. Area density and numbers of macrophages were evaluated with an image analysis board and software developed in our laboratory. This immunohistochemically characterized subpopulation of macrophages was rarely found in the peri- and paravascular connective tissue of several organs of normocholesterolemic rabbits, but significantly increased in the heart and intimal hyperplasias, and to a lower extent in other organs of hypercholesterolemic rabbits. In conclusion, quantitative fluorescence immunohistochemistry was used to characterize a subpopulation of macrophages, which seem to respond specifically to hypercholesterolemia.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ball, R.Y., Carpenter, K.L., and Mitchinson, M.J., 1987, What is the significance of ceroid in human atherosclerosis? Arch Pathol Lab Med 111: 1134–1140
Cathcart, M.K., McNally, A.K., Morel, D.W., and Chisolm, G.M., 1989, Superoxide anion participation in human monocyte-mediated oxidation of low-denity lipoprotein and conversion of low-density lipopro-tein to a cytotoxin. J Immunol 142: 1963–1969
Couffinhal, T., Duplaa, C., Labat, L., Moreau, C., Bietz, I., and Bonnet, J., 1993, Effect of low density lipoprotein on monocyte adhesiveness to endothelial cells in vitro. Atherosclerosis 99: 35–45
Daar, A. S., Fuggle, S. V., Hart, D. N. J., Dalchau, R., Abdulaziz, Z., Fabre, J. W., Ting, A., and Morris, P. J., 1983, Demonstration and phenotypic characterization of HLA-DR positive interstitial dendritic cells widely distributed in human connective tissues. Transplant Proc 15: 311–315
Darley-Usmar, M., Severn, A., O’Leary, V.J., and Rogers, M., 1991, Treatment of macrophages with oxidized low-density lipoprotein increases their intracellular glutathione content. Biochem J 278: 429–434
Del Rio, L.A., Sandalio, L.M., and Palma, J.M., 1990, A new cellular function for peroxisomes related to oxygen free radicals? Experentia 46: 989–992
Dhaunsi, G. S., Singh, I., and Hanevold, C.D., 1993, Peroxisomal participation in the cellular response to the oxidative stress of endotoxin. Mol Cell Biochem 126: 25–35
Henning, B., and Chow, C.K., 1988, Lipid peroxidation and endothelial cell injury: Implications in atherosclerosis. Free Radical Biol. Med 4: 99–106
Hoffman, R., Brook, G.J., and Aviram, M., 1992, Hypolipidemic drugs reduce lipoprotein susceptibility to undergo lipid peroxidation: in vitro and ex vivo studies. Atherosclerosis 93: 105–113
Janssen, Y.M., Van Houten, B., Borm, P.J., and Mossman, B.T., 1993, Cell and tissue responses to oxidative damage. Lab Invest 69: 261–274
Kaesberg, B., Harrach, B., Dieplinger, H., and Robenek, H., 1993, In situ immunolocalization of lipoproteins in human arteriosclerotic tissue, Arterioscler Thromb 13: 133–146
Kinscherf, R., Köhler, C., Kreuter, C., Pill, J., and Metz, J., 1995, Hypercholesterolemia increases manganese Superoxide dismutase immunoreactive macrophages in myocardium. Histochem Cell Biology
Labarrere, C. A., McIntyre, J. A., Halbrook, H., and Faulk, W. P., 1991, Major histocombatibility antigens in transplanted human hearts before perfusion. J Heart Lung Transplant 10: 409–415
Rankin, S.M., Parthasarathy, S., and Steinberg, D., 1991, Evidence for a dominant role of lipoxygenase(s) in the oxydation of LDL by peritoneal macrophages. J Lip Res 32: 449–456
Rosenfeld, M.E., 1991, Oxidized LDL affects multiple atherogenic cellular responses. Circulation 83: 2137–2140
Rosenfeld, M.E., Khoo, J.C., Miller, E., Parthasarathy, S., Palinski, W., and Witztum, J.L., 1991, Macrophage derived foam cells freshly isolated from rabbit atherosclerotic lesions degrade lipoproteins, promote oxidation of low-density lipoproteins, and contain oxidation-specific lipid-protein adducts. J Clin Invest 87: 90–99.
Singal, P.K., Dhalla, A.K., Hill, M., and Thomas, T.P., 1993, Endogenous antioxidant changes in the myocardium in response to acute and chronic stress conditions. Mol Cell Biochem 129: 179–186
Stadius, M.L., Rowan, R., Fleischhauer, J.F., Kernhoff, R., Billingham, M., and Gown, A.M., 1992, Time course and cellular characteristics of the iliac artery response to acute balloon injury. Arterioscler Thromb 12: 1267–1273
Stevens, J.B., and Autor, A.P., 1977, Oxygen-induced synthesis of Superoxide dismutase and catalase in pulmonary macrophages of neonatal rats. Lab Invest 37: 470–478
Tsukada, T., Rosenfeld, M., Ross, R., and Gown, A.M., 1986, Immunocytochemical analysis of cellular components in atherosclerotuc lesions: Use of monoclonal antibodies with the Watanabe and fat-fed rabbit. Arteriosclerosis 6: 601–613
Witztum, J.L., 1990, The role of monocytes and oxidized LDL in atherosclerosis. Atherosclerosis News 21: 59–69
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Metz, J., Pavlov, P., Kinscherf, R., Köhler, C., Usinger, C. (1996). Quantification of Macrophages in the Cardiovascular System of Hypercholesterolemic Rabbits by Use of Digital Image Processing. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_35
Download citation
DOI: https://doi.org/10.1007/978-1-4899-1866-6_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-1868-0
Online ISBN: 978-1-4899-1866-6
eBook Packages: Springer Book Archive