Abstract
The optical microscope, arguably the most mature of all laboratory instruments, is currently enjoying a period of intense development that is opening up many new application areas. These developments have been made possible by the use of new optical arrangements, sophisticated light sources and detectors and by the application of novel fluorescence-based contrast forms. The combination of fluorescence with microscopy yields detailed and selective information at the microscopic level. For instance, different structural and functional entities in biological cells can be stained selectively with (extrinsic) fluorescent probes so that they can be easily identified in the images. Numerous fluorescent probe molecules are now available which enable the selective imaging of the electric potential of membranes, DNA, spatial variations of free ion concentrations, and specific proteins within single (living) biological cells.
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© 1996 Springer Science+Business Media New York
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Gerritsen, H.C. (1996). Confocal Fluorescence Lifetime Imaging. In: SlavÃk, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_3
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DOI: https://doi.org/10.1007/978-1-4899-1866-6_3
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