Abstract
Fluorescence lifetime-resolved imaging microscopy — FLIM — is a relatively new fluorescence imaging technique by which the temporal attributes of luminescence emission can be measured directly at each location of a microscope image. The mean lifetime of the emission can be determined at every pixel of a digital image with a high spatial and temporal resolution. Different fluorescence components with differing decay times can be enhanced or suppressed throughout a fluorescence image. The measurements can be carried out at every pixel simultaneously. In this presentation a account of the experiment is given in terms of a comprehensive integrated theoretical framework.
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Clegg, R.M., Schneider, P.C. (1996). Fluorescence Lifetime-Resolved Imaging Microscopy: A General Description of Lifetime-Resolved Imaging Measurements. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_2
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DOI: https://doi.org/10.1007/978-1-4899-1866-6_2
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