Abstract
The application of pH-sensitive fluorescent probes represents both a progressive and an easy way of in vivo determination of cytoplasmic pH values. The ratio microscopy can deliver pH values of a large number of cells simultaneously. This illustrates the tremendous progress of the pH-measuring techniques, as 31P NMR, ion-selective microelectrodes, distribution of weak acids or destruction (permeabilization) of cells yielded only one single value from each measurement procedure.
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Cimprich, P., Slavík, J. (1996). Distribution of Individual Cytoplasmic pH Values in a Cell Suspension. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_11
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DOI: https://doi.org/10.1007/978-1-4899-1866-6_11
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