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Models for Assessing Drug Absorption and Metabolism pp 325–346Cite as

Models for Investigation of Peptide and Protein Transport across Cultured Mammalian Respiratory Epithelial Barriers

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Part of the book series: Pharmaceutical Biotechnology ((PBIO,volume 8))

Abstract

In this chapter, we describe in vitro methods that can be used for the investigation of peptide and protein transport across respiratory epithelial barriers. We begin with the approaches for culture of alveolar epithelial and tracheo(bronchial) epithelial cells, followed by experimental methods to investigate transport and metabolism utilizing the cultured cells. Where appropriate, advantages and disadvantages of the approaches utilized are discussed.

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Authors and Affiliations

  1. Departments of Medicine, Physiology and Biophysics, and Biomedical Engineering, Schools of Medicine and Engineering, University of Southern California, Los Angeles, California, 90033, USA

    Kwang-Jin Kim

  2. Departments of Medicine and Pathology, School of Medicine, University of Southern California, Los Angeles, California, 90033, USA

    Edward D. Crandall

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  1. Kwang-Jin Kim
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  1. The University of Kansas, Lawrence, Kansas, USA

    Ronald T. Borchardt

  2. SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania, USA

    Philip L. Smith

  3. Tacora Corporation, Seattle, Washington, USA

    Glynn Wilson

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Kim, KJ., Crandall, E.D. (1996). Models for Investigation of Peptide and Protein Transport across Cultured Mammalian Respiratory Epithelial Barriers. In: Borchardt, R.T., Smith, P.L., Wilson, G. (eds) Models for Assessing Drug Absorption and Metabolism. Pharmaceutical Biotechnology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1863-5_17

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