Abstract
Escherichia coli strains belonging to 0103:K-:H2 serovar and rhamnose negative biovars are responsible for frequent life-threatening diarrheas in weaned rabbits from national breeding units in western Europe (Blanco et al., 1994; Camguilhem and A. Milon, 1989). According to their mechanisms of pathogenesis, these strains may be considered as enteropathogenic E. coli (EPEC)-like. They adhere to intestinal brush border and to HeLa cells by mean of an adhesin called AF/R2 (Adhesive Factor/Rabbit 2) (Milon et al., 1990). They do not produce known toxins (i.e. ST, LT, SLT, CNF, CLDT) (Blanco et al., 1994; Mariani-Kurkdjian et al., 1993) and bear sequences homologous to EPEC eaeA (Leroy et al., 1994). Recently, a new phenotype has been identified based on an in vitro test that includes a short contact between bacterias and HeLa cells, followed by washes and incubation of cells in presence of gentamicin. After several days, cells treated by 0103 strains develop a cytopathic effect (CPE): apparition of giant cells with a heavy stress fibers cytoplasmic network.
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References
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© 1997 Springer Science+Business Media New York
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Chalareng, C., Pillien, F., Boury, M., Tasca, C., De Rycke, J., Milon, A. (1997). AF/R2 Adhesin and Cytopathic Effect as Virulence Traits of Diarrhea-Inducing Escherichia coli O103 in European Rabbit. In: Paul, P.S., Francis, D.H., Benfield, D.A. (eds) Mechanisms in the Pathogenesis of Enteric Diseases. Advances in Experimental Medicine and Biology, vol 412. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1828-4_44
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DOI: https://doi.org/10.1007/978-1-4899-1828-4_44
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