Summary
Transmissible gastroenteritis (TGE) is an enteric disease of swine caused by a coronavirus, designated as transmissible gastroenteritis virus (TGEV). Commonly used methods for TGEV detection include viral isolation and detection of the viral antigen by indirect immunofluorescence (IFA), immunoperoxidase, and immunogold silver staining. Each of these techniques has some advantages and disadvantages. In general IFA and immunohistochemistry are preferred over viral isolation as TGEV isolation is not very reliable because not all field isolates replicate in cell cultures. The diagnosis of TGEV has become more complicated since the emergence of porcine respiratory coronavirus (PRCV). PRCV is believed to be a TGEV mutant, and can not be easily differentiated from TGEV by immunological tests. Nucleic acid probes and polymerase chain reaction (PCR) have successfully been used to detect and differentiate these viruses. These techniques can detect viral nucleic acids in the specimen but do not provide information on the cell types infected by these viruses. Recently we have developed isotopic and nonisotopic in situ hybridization techniques (ISH) for the detection of these viral nucleic acids in formalin-fixed paraffin-embedded tissues. Furthermore, this procedure can differentiate between TGEV-and PRCV-infected cells. By ISH, TGEV is detected in the mature absorptive enterocytes of tissues infected by TGEV and the crypt epithelial cells are also infected but to a lesser extent. For PRCV, the main infected cells are epithelial cells of the bronchioles, type II pneumocytes, and alveolar and septal macrophages. ISH is an excellent tool for studying molecular pathogenesis of these two viruses especially when used in combination with immunohistochemistry.
Keywords
- Viral Nucleic Acid
- Direct Fluorescence Antibody
- Bronchiolar Epithelial Cell
- Transmissible Gastroenteritis Virus
- Feline Infectious Peritonitis Virus
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Bohl, E. H., and Pensaert, M. B., 1989, Transmissible gastroenteritis virus (classical enteric variant) and transmissible gastroenteritis virus (respiratory variant). In: M. B. Pensaert (Ed), Virus infections of porcines, Elsevier Science Publishers B.N., Amsterdam, pp. 139–165.
Britton, P., Mawditt, K. L., and Page, K. W., 1991, The cloning and sequencing of the virion protein genes from a British isolate of porcine respiratory coronavirus: comparison with transmissible gastroenteritis virus genes, Virus Res. 21: 181–198.
Britton, P., and Page, K. W., 1990, Sequence of the S gene from a virulent British field isolate of transmissible gastroenteritis virus, Virus Res. 18: 71–80.
Callebaut, P., Correa, I., Pensaert, M., et al., 1988, Antigenic differentiation between transmissible gastroenteritis virus of swine and a related porcine respiratory coronavirus, J. Gen. Vim!. 69: 1725–1730.
Chu, R. M., Li, N. J., Glock, R. D., and Ross, R. F., 1982, Application of peroxidase-antiperoxidase staining technique for detection of transmissible gastroenteritis virus in pigs, Am. J. Vet. Res. 43: 77–81.
Cox, E., Hooybergh, J., and Pensaert, M. B., 1990, Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus, Res. Vet. Sci. 48: 165–169.
Delmas, B., Gelfi, J., and L’Haridon, R., et al., 1992, Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV, Nature. 357: 417–420.
Doyle, L. P., and Hutchings, L. M., 1946, A transmissible gastroenteritis in pigs, J. Am. Vet. Med. Assoc. 08: 257–259.
Frederick, G. I., Bohl, E. H., and Cross, J. E., 1976, Pathogenicity of an attenuated strain of transmissible gastroenteritis virus for newborn pigs, Am. J. Vet. Res. 42: 1163–1169.
Halbur, P. G., Paul, P. S., Vaughn, E. M., and Andrews, J. J., 1993, Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310, J. Vet. Diagn. Invest. 5: 184–188.
Halbur, R. G., Andrews, J. J., Huffman, E. L., et al., 1994, Development of a streptavidin-biotin immunoperoxidase for the detection of porcine reproductive and respiratory syndrome virus antigen in porcine lung, J. Vet. Diagn. Invest. 6: 254–257.
Hill, H. T., Biwer, J. D., Woods, R. D., and Wesley, R. D., 1989, Porcine respiratory coronavirus isolated from two U.S. swine herds, Proc. Am. Assoc. Swine. Pract. 333–335.
Horzinek, M. C., Lutz, H., and Pedersen, N. C., 1982, Antigenic relationships among homologous structure polypeptides of porcine, feline, and canine coronaviruses, Infect. Immun. 37: 1148–1155.
Jackwood, D. J., Bae, I., Jackwood, R. J. et al., 1993, Transmissible gastroenteritis virus and porcine respiratory coronavirus molecular characterization of the S gene using cDNA probes and nucleotide sequence analysis, Adv. Exp. Med. Biol. 342: 43–48.
L., Hemperly, J. J., and Lloyd, R. V., 1991, Expression of neural cell adhesion molecule in normal and neo-plastic human neuroendocrine tissues, Am. J. Pathol. 138: 961–969.
La Bonnardiere, C., and Laude, H., 1983, Interferon induction in rotavirus and coronavirus infections: a review of recent results, Ann. Rech. Vet. 14: 507–511.
Larochelle, R., and Mogar, R., 1993, The application of immunogold silver staining (IGSS) for the detection of the transmissible gastroenteritis virus in fixed tissue, J. Vet. Diagn. Invest. 5: 16–20.
Laude, H., Van Reeth, K., and Pensaert, M., 1993, Porcine respiratory coronavirus: molecular features and virus-host interactions, Vet.Res. 24: 125–150.
Morin, M., Morehouse, L. G., Solorzano, R. F., and Olsen, L. D., 1973, Transmissible gastroenteritis in feeder swine: clinical, immunofluorescence and histopathological observations, Can. J. Comp. Med. 37: 239–248.
O’Toole, D., Brown, I., Bridges, A., and Cartwright, S. F., 1989, Pathogenicity of experimental infection with `pneumotropic` porcine respiratory coronavirus, Res. Vet. Sci. 47: 23–29.
Paul, R. S., Halbur, R. G., and Vaughn, E. M., 1994, Significance of porcine respiratory coronavirus infection, Compend. Cont. Educ. Pract. Vet. 16: 1223–1234.
Paul, R. S., Vaughn, E. M., and Halbur, P. G., 1992, Characterization and pathogenicity of a new porcine respiratory coronavirus strain AR310, Proc. Int. Pig. Vet. Soc. Congr. 12: 92
Pensaert, M. B., Callebaut, R, and Vergote, J., 1986, Isolation of a porcine respiratory non-enteric coronavirus related to transmissible gastroenteritis, Vet. Quart. 8: 257–261.
Pensaert, M. B., Haelterman, E. O., and Hinsman, E. J., 1970, Transmissible gastroenteritis of swine: Virus-intestinal cell interactions. 11. Electron microscopy of the epithelium in isolated jejunal loops, Arch. Gesamte. Virusforsch. 31: 335–351.
Rassachaert, D., Duarte, M., and Laude, H., 1990, Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions, J. Gen. Virol. 71: 2599–2607.
Rassachaert, D., and Laude, H., 1987, The predicted primary structure of the peplomer protein E2 of the porcine coronavirus transmissible gastroenteritis virus, J. Gen. Virol. 68: 1883–1890.
Rossen, J. W., Bekker, C. R, Voorhout, W. F. and et al., 1994, Entry and release of transmissible gastroenteritis coronavirus are restricted to apical surfaces of polarized epithelial cells, J. Virol. 68: 7966–7973.
Saif, L. J., and Wesley, R. D., 1994, Transmissible gastroenteritis, In: A. D. Leman, B. E. Strauss, W. L. Mengeling, S. D. Allaire, D. J. Taylor (Eds), Diseases of swine, 7th ed., Iowa State University Press, Ames, IA, pp. 362–386.
Sanchez, C. M., Gebauer, F., Sune, C., and Mendez, A. et al., 1992, Genetic evolution and tropism of transmissible gastroenteritis coronavirus, Virol. 190: 92–105.
Sherpherd, R. W., Butler, D. G., Cutz, E., and Gall, D. G., 1979, The mucosal lesion in viral enteritis: extent and dynamics of the epithelial response to virus invasion in transmissible gastroenteritis of piglets, Gastroenterology 76: 770–777.
Shockley, L. J., Kapke, P. A., Lapps, W., and Brian, D. A. et al., 1987, Diagnosis of porcine and bovine enteric coronavirus infections using cloned cDNA probes, J. Clin. Micro. 25: 1591–1596.
Sirinarumitr, T., Paul, R S., Kluge, J. P., and Halbur, P. G., 1996, In situ hybridization technique for the detection of swine enteric and respiratory coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in the formalin-fixed paraffin-embedded tissues, J. Virol. Methods. 56: 149–160.
Spaan, W., Cavanagh, D., and Horzinek, M. C., 1988, Coronaviruses: structure and genome expression, J. Gen. Viro1. 69: 2939–2952.
Thake, D. C., 1968, Jejuna! epithelium in transmissible gastroenteritis of swine (an electron microscopic and histochemical study), Am. J. Pathol. 53: 149–168.
Van Nieustadt, A. P., and Pol, J. M. A., 1989, Isolation of a TGE virus-related respiratory coronavirus causing fatal pneumonia in pig, Vet. Rec. 124: 43–44.
Vaughn, E. M., Halbur, P. G., and Paul, P. S., 1996, The use of nonradioactive cDNA probes to differentiate porcine respiratory coronavirus and transmissible gastroenteritis virus isolates, J. Vet. Diagn. Invest.
Vaughn, E. M., Halbur, P. G., and Paul, R. S., 1995, Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, 3–1 genes, J. VIrol. 69: 3176–3184.
Vaughn, E. M., Halbur, R. G., and Paul, P. S., 1994, Three new isolates of porcine respiratory coronavirus with various pathogenicities and spike (S) gene deletions, J. Clin. Microbiol 69: 1809–1812.
Vaughn, E. M., and Paul, P. S., 1993, Antigenic and biological diversity among transmissible gastroenteritis virus isolates of swine. Vet. Microbial. 36: 333–347.
Wagner, J. E., Beamer, R D., and Restic, M., 1973, Electron microscopy of intestinal epithelial cells of piglets infected with a transmissible gastroenteritis virus, Can. J. Comp. Med. 37: 177–188.
Wesley, R. D., Woods, R. D., Hill, H. T., and Biwer, J. D., 1990, Evidence for a respiratory coronavirus antigenically similar to transmissible gastroenteritis in the United States, J. Vet. Diagn. Invest. 2: 312–317.
Wesley, R. D., Woods, R. D., and Cheung, A. K., 199la, Genetic basis for the pathogenesis of transmissible gastroenteritis virus, J. l Irol. 64: 4761–4768.
Wesley, R. D., Wesley, I. V., and Woods, R. D., 199 lb, Differentiation between transmissible gastroenteritis virus and porcine respiratory coronavirus using a cDNA probe, J. Vet. Diagn. Invest. 3: 29–32.
Weingartl, H., and Derbyshire, J. B., 1994, Evidence of a putative second receptor for porcine transmissible gastroenteritis virus on the villous enterocytes of newborn pigs, J. Viro!. 68: 7253–7259.
Weingartl, H., and Derbyshire, J. B., 1993, Binding of porcine transmissible gastroenteritis virus by enterocytes from newborn and weaned piglets, Vet. Microbiol. 35: 1163–1169.
Woods, R. D., Cheville, N. F., and Gallagher, J. E., 1981, Lesions in the small intestines of newborn pigs inoculated with porcine, feline, and canine coronavirus, Am. J. Vet. Res. 42: 1163–1169.
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Sirinarumitr, T., Paul, P.S., Halbur, P.G., Kluge, J.P. (1997). An Overview of Immunological and Genetic Methods for Detecting Swine Coronaviruses, Transmissible Gastroenteritis Virus, and Porcine Respiratory Coronavirus in Tissues. In: Paul, P.S., Francis, D.H., Benfield, D.A. (eds) Mechanisms in the Pathogenesis of Enteric Diseases. Advances in Experimental Medicine and Biology, vol 412. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1828-4_4
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