Abstract
Typical serologic schemes for identification of E. coli 0157:H7 are based on confirmation of both gene products. The present preliminary study was designed to compare genomic evidence of the H7 gene with serologic results. Published sequence in the variable central region of the flagellar antigen (Schoenhals and Whitfield, 1993) was used to develop a PCR test for the H7 gene. Amplicons of 220 and 345 bp were generated by our primer set; these occurred either individually or in combination. Presence of H7 confirmed in this manner was in general concordance with serologic findings, although exceptions occurred. Hybridization of labeled 345 bp amplicon to Southern blots of E. coli Barn H1 digests revealed a single band in isolates determined as H7 by PCR.
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Schoenhals, G., and Whitfield, C., 1993, Comparative Analysis of Flagellin Sequences from Escherichia coli Strains Possessing Serologically Distinct Flagellar Filaments with a Shared Complex Surface Pattern, J. of Bacteriology., 175: 5395–5402.
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© 1997 Springer Science+Business Media New York
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Bailey, C.W., Carson, C.A. (1997). Variation in Manifestation of E. coli H7 Antigen. In: Paul, P.S., Francis, D.H., Benfield, D.A. (eds) Mechanisms in the Pathogenesis of Enteric Diseases. Advances in Experimental Medicine and Biology, vol 412. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1828-4_11
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DOI: https://doi.org/10.1007/978-1-4899-1828-4_11
Publisher Name: Springer, Boston, MA
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